葡萄胚挽救技术优化及无核和玫瑰香味新种质创制

doi:10.3864/j.issn.0578-1752.2020.16.012

Abstract

Abstract:

【Objective】The aim of this study was to investigate the grape scent traits to provide important material basis for seedless aroma grape breeding. By exploring the parental genotype, embryo development morphology and growth regulator on embryo rescue efficiency, further optimizing the seedless × muscat scent combined embryo rescue system, and molecular marker technology was used to initially identify seedless traits of hybrid offspring.【Method】Headspace solid phase micro-extraction (HS-SPME) combined with gas chromatography-mass spectrometry technique was used to determine the content of aromatic substances in the grapes of 10 different muscat-scented and non-muscat scented varieties. Excellent aroma varieties were selected as parents, then combined with the previous research results of the group, 13 cross-combinations, including 6 female parents and 5 male parents, were used to obtain new grapevines with seedlessness and aroma. Under aseptic conditions, the ovules were cultured from young fruits in the dark for 8 weeks. The developed hybrid embryos were inoculated in a variety of embryo germination media, with different concentrations and different proportions of hormones to assess and improve the germination rate and germination seedling rate. After the embryo developed into plants, they were placed in the greenhouse for hardening, the survived F₁ generation was transplanted to the field. The molecular marker technology was used to select the seedless characters of F₁hybrid lines in the early stage. 【Result】Among 10 table grapes, it was the terpenes that determined the aroma of muscat, which could be detected in 8 muscat scent varieties, and the content of which ranged from 0.0246 to 1.3824. Based on the above evaluation, ‘Muscat of Alexandria’, ‘Shine-Muscat’ and ‘Muscat Hamburg’ were selected to create new seedless aroma germplasm because their high levels of terpenoids. The study showed 1 284 progeny were obtained by embryo rescue technique, and 697 of them survived after transplanting into field. Ruby Seedless and Flame Seedless with a higher seedling rate were suitable as female parent in embryo rescue. Muscat Hamburg and Shine-Muscat proved as the better male parents. Among them, the embryo development rate and seedling formation rate of Ruby seedless × Aishenmeigui were the highest, at 48.59% and 51.71%, respectively. The germination rate was the highest when embryos were inoculated into WPM as based medium, and the growth regulator (1.0 mg?L^-1 KT + 0.5 mg?L^-1 NAA + 1.0 mg?L^-1 ZT) was added, which was 11.33%, higher than the control which was 7.41%. Three molecular markers GLSP1-569, SCF27-2000 and SCC8-1018 were selected for detecting the cross parents and hybrid progenies. The results showed that 27 out of 101 hybrid strains had seedless genes. 【Conclusion】 Muscat of Alexandria and Sunshine-Muscat had a high scent content, and the embryo rescue efficiency was high after crossing with European grapes, which was a suitable scented parent material. Ruby Seedless and Flame Seedless was suitable as female parent. During embryo germination and culture, using WPM medium as the basic medium, adding appropriate concentrations of KT and ZT had a better effect of promoting root growth. The seedless traits of hybrid F₁ generation were detected by molecular markers, and the seedless rate was 26.73%. At present, few aroma markers have been developed, so the hybrid offspring obtained in this study could provide an important material basis for the further study of aroma gene markers.

Key words: grapevine, aromatic characteristic, embryo rescue, marker-assisted selection, seedlessness

JIA ShanShan,LUO QiangWei,LI ShaSha,WANG YueJin. Optimization of Embryo Rescue Technique and Production of Potential Seedless Grape Germplasm with Rosy Aroma[J].Scientia Agricultura Sinica, 2020, 53(16): 3344-3355.

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Figures/Tables 10

Table 1

Cross combination configuration"

杂交组合（母本×父本） Cross combination (♀×♂)	母本Female parent		父本Male parent
杂交组合（母本×父本） Cross combination (♀×♂)	种或杂种 Species or hybrid	性状 Characteristic	种或杂种 Species or hybrid	性状 Characteristic
爱神玫瑰×玫瑰香 Aishenmeigui × Muscat Hamburg	欧亚种 V. vinifera	无核、玫瑰香味 Stenospermic, Muscat-scented	欧亚种 V. vinifera	有核、玫瑰香味 Seeded, Muscat-scented
爱神玫瑰×阳光玫瑰 Aishenmeigui × Shine-Muscat	欧亚种 V. vinifera	无核、玫瑰香味 Stenospermic, Muscat-scented	欧美杂种 V. labruscana ×V. vinifera	有核、玫瑰香味 Seeded, Muscat-scented
爱神玫瑰×昆香无核 Aishenmeigui × Kunxiang Seedless	欧亚种 V. vinifera	无核、玫瑰香味 Stenospermic, Muscat-scented	欧亚种 V. vinifera	无核、玫瑰香味 Stenospermic, Muscat-scented
火焰无核×红亚历山大 Flame Seedless × Red Alexander	欧亚种 V. vinifera	无核 Stenospermic	欧亚种 V. vinifera	有核、玫瑰香味 Seeded, Muscat-scented
火焰无核×阳光玫瑰 Flame Seedless × Shine-Muscat	欧亚种 V. vinifera	无核 Stenospermic	欧美杂种 V. labruscana ×V. vinifera	有核、玫瑰香味 Seeded, Muscat-scented
火焰无核×新郁 Flame Seedless × Xinyu	欧亚种 V. vinifera	无核 Stenospermic	欧亚种 V. vinifera	有核、玫瑰香味 Seeded, Muscat-scented
昆香无核×爱神玫瑰 Kunxiang Seedless × Aishenmeigui	欧亚种 V. vinifera	无核、玫瑰香味 Stenospermic, Muscat-scented	欧亚种 V. vinifera	无核、玫瑰香味 Stenospermic, Muscat-scented
昆香无核×玫瑰香 Kunxiang Seedless × Muscat Hamburg	欧亚种 V. vinifera	无核、玫瑰香味 Stenospermic, Muscat-scented	欧亚种 V. vinifera	有核、玫瑰香味 Seeded, Muscat-scented
克瑞森无核×玫瑰香 Crimson Seedless × Muscat Hamburg	欧亚种 V. vinifera	无核 Stenospermic	欧亚种 V. vinifera	有核、玫瑰香味 Seeded, Muscat-scented
克瑞森无核×新郁 Crimson Seedless × Xinyu	欧亚种 V. vinifera	无核 Stenospermic	欧亚种 V. vinifera	有核、玫瑰香味 Seeded, Muscat-scented
红宝石无核×玫瑰香 Ruby Seedless × Muscat Hamburg	欧亚种 V. vinifera	无核 Stenospermic	欧亚种 V. vinifera	有核、玫瑰香味 Seeded, Muscat-scented
红宝石无核×红亚历山大 Ruby Seedless × Red Alexander	欧亚种 V. vinifera	无核 Stenospermic	欧亚种 V. vinifera	有核、玫瑰香味 Seeded, Muscat-scented
红宝石无核×爱神玫瑰 Ruby Seedless × Aishenmeigui	欧亚种 V. vinifera	无核 Stenospermic	欧亚种 V. vinifera	无核、玫瑰香味 Stenospermic, Muscat-scented

Table 1

Table 2

Table 3

Table 4

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Table 5

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References 37

[1]	LI S S, LI Z Y, ZHAO Y N, ZHAO J, LUO Q W, WANG Y J. New disease-resistant, seedless grapes are developed using embryo rescue and molecular markers. 3 Biotech, 2019,10(1):4. pmid: 31824815
[2]	LI T M, LI Z Q, YIN X, GUO Y R, WANG Y J, XU Y. Improved in vitro Vitis vinifera L. embryo development of F₁ progeny of 'Delight'×'Ruby seedless’ using putrescine and marker-assisted selection. In Vitro Cellular & Developmental Biology-Plant, 2018,54(3):291-301.
[3]	LI J, WANG X H, WANG X P, WANG Y J. Embryo rescue technique and its applications for seedless breeding in grape. Plant Cell Tissue and Organ Culture, 2015,120(3):861-880. doi: 10.1007/s11240-014-0656-4
[4]	王爱玲, 王跃进, 唐冬梅, 张剑侠, 张朝红. 提高无核葡萄胚挽救中幼胚成苗率的研究. 中国农业科学, 2010,43(20):4238-4245.
	WANG A L, WANG Y J, TANG D M, ZHANG J X, ZHANG C H. Research on improvement of seedling rate in embryo rescue of seedless grapes. Scientia Agricultura Sinica, 2010,43(20):4238-4245. (in Chinese)
[5]	唐冬梅, 王跃进, 赵荣华, 潘学军, 蔡军社, 张剑侠, 张朝红, 骆强伟. 无核葡萄胚挽救中影响胚发育的因子. 中国农业科学, 2009,42(7):2449-2457.
	TANG D M, WANG Y J, ZHAO R H, PAN X J, CAI J S, ZHANG J X, ZHANG C H, LUO Q W. Factors influencing embryo development in embryo rescue of seedless grapes. Scientia Agricultura Sinica, 2009,42(7):2449-2457. (in Chinese)
[6]	李莎莎, 王跃进. 葡萄无核基因及无核育种研究进展. 园艺学报, 2019,46(9):1711-1726.
	LI S S, WANG Y J. Advances in seedless gene researches and seedless breeding in grapevine. Acta Horticulturae Sinica, 2019,46(9):1711-1726. (in Chinese)
[7]	唐冬梅. 无核葡萄杂交胚挽救新种质创建与技术完善[D]. 杨凌: 西北农林科技大学, 2010.
	TANG D M. Novel germplasm innovation of seedless grapes by embryo rescue and technique improvement[D]. Yangling: Northwest A & F University, 2010. (in Chinese)
[8]	TIAN L L, WANG Y J, NIU L, TANG D M. Breeding of disease-resistant seedless grapes using Chinese wild Vitis spp.: I. In vitro embryo rescue and plant development. Scientia Horticulturae, 2008,117(2):136-141.
[9]	史文静, 骆强伟, 王跃进. 无核香味葡萄胚挽救育种研究. 西北植物学报, 2018,38(6):983-993.
	SHI W J, LUO Q W, WANG Y J. Breeding grapevine varieties for seedlessness with flavour using embryo rescue. Acta Botanica Boreali-Occidentalia Sinica, 2018,38(6):983-993. (in Chinese)
[10]	PAWLISZYN J, PAWLISZYN B, PAWLISZYN M. Solid phase microextraction (SPME). The Chemical Educator, 1997,2(4):1-7.
[11]	PERESTRELO R, BARROS A S, ROCHA S M, CÂMARA J S. Optimisation of solid-phase microextraction combined with gas chromatography-mass spectrometry based methodology to establish the global volatile signature in pulp and skin of Vitis vinifera L. grape varieties. Talanta, 2011,85(3):1483-1493. pmid: 21807213
[12]	STEPHAN A, BUCKING M, STEINHART H. Novel analytical tools for food flavours. Food Research International, 2000,33(3/4):199-209.
[13]	魏玲玲, 王武, 陶建敏. 葡萄香气物质研究进展. 中国南方果树. 2018,47(3):159-165.
	WEI L L, WANG W, TAO J M. Research progress of aroma substances in grape. South China Fruits, 2018,47(3):159-165. (in Chinese)
[14]	GUO D L, ZHAO H L, LI Q, ZHANG G H, JIANG J F, LIU C H, YU Y H. Genome-wide association study of berry-related traits in grape [Vitis vinifera L.] based on genotyping-by-sequencing markers. Horticulture Research, 2019,6:11. pmid: 30603096
[15]	DUEHOLM B, DREW D P, SWEETMAN C, SIMONSEN H T. In planta and in silico characterization of five sesquiterpene synthases fromVitis vinifera(cv. Shiraz) berries. Planta, 2019,249:59-70. doi: 10.1007/s00425-018-2986-7 pmid: 30136197
[16]	WU Y S, DUAN S Y, ZHAO L P, GAO Z, LUO M, SONG S R, XU W P, ZHANG C X, MA C, WANG S P. Aroma characterization based on aromatic series analysis in table grapes. Scientific Reports, 2016,6(1):31116.
[17]	RUIZ-GARCÍAA L, HELLÍN P, FLORES P, FENOLL J. Prediction of Muscat aroma in table grape by analysis of rose oxide. Food Chemistry, 2014,15(4):151-157.
[18]	WU Y S, ZHANG W W, SONG S R, XU W P, ZHANG C X, MA C, WANG L, WANG S P. Evolution of volatile compounds during the development of Muscat grape ‘Shine Muscat' (Vitis labrusca ×V. vinifera). Food Chemistry, 2020,309:125778. pmid: 31704071
[19]	樊秀彩, 张颖, 姜建福, 孙海生, 李民, 刘崇怀. 近20年来国外鲜食葡萄品种选育进展. 中外葡萄与葡萄酒, 2012(2):53-59.
	FAN X C, ZHANG Y, JIANG J F, SUN H S, LI M, LIU C H. Progress in the breeding of fresh grape varieties abroad in recent 20 years. Sino-Overseas Grapevine & Wine, 2012(2):53-59. (in Chinese)
[20]	李铁梅. 'DR'优系软核性状改良及玫瑰香型无核葡萄新种质创制[D]. 杨凌: 西北农林科技大学, 2014.
	LI T M. Soft seed traits improvement of ‘DR’ lines and novel germplasm innovation of aroma of muscat seedless grapes[D]. Yangling: Northwest Agriculture and Forestry University, 2014. (in Chinese)
[21]	谭伟, 唐晓萍, 董志刚, 李晓梅. 4个无核鲜食葡萄品种及其亲本果实香气成分分析. 果树学报, 2015,32(3):440-447.
	TAN W, TANG X P, DONG Z G, LI X M. Analysis on fruit aromatic compounds of four seedless grape and their parents. Journal of Fruit Science, 2015,32(3):440-447. (in Chinese)
[22]	屈田田, 张剑侠, 骆强伟, 王跃进. 无核葡萄抗寒抗病胚挽救育种应用研究. 果树学报, 2017,34(2):157-165.
	QU T T, ZHANG J X, LUO Q W, WANG Y J. A Study on the application of seedless grapevine breeding for cold-hardness and disease-resistance using embryo rescue. Journal of Fruit Science, 2017,34(2):157-165. (in Chinese)
[23]	刘巧. 利用胚挽救技术培育抗寒无核葡萄新种质[D]. 杨凌: 西北农林科技大学, 2015.
	LIU Q. Breeding new, cold-resistant, seedless grape germplasm using embryo rescue technology[D]. Yangling: Northwest A&F University 2015. (in Chinese)
[24]	PARK S K, MORRISON J C, ADAMS D O, NOBLE A C. Distribution of free and glycosidically bound monoterpenes in the skin and mesocarp of Muscat of Alexandria grapes during development. Journal of Agricultural and Food Chemistry, 1991,39(3):514-518.
[25]	李华. 葡萄的芳香物质. 中外葡萄与葡萄酒, 2001(6):43-44.
	LI H. The flavor component of grapes. Sino-Overseas Grapevine & Wine. 2001(6):43-44. (in Chinese)
[26]	谭伟, 李晓梅, 唐晓萍, 董志刚. 鲜食葡萄杂交后代果实玫瑰香味表型的遗传倾向初报. 中外葡萄与葡萄酒, 2013(4):6-9.
	TAN W, LI X M, TANG X P, DONG Z G. Preliminary study on the inheritance of the rose fragrance phenotype in hybrids of table grapes. Sino-Overseas Grapevine & Wine, 2013(4):6-9. (in Chinese)
[27]	张剑侠, 牛茹萱. 无核葡萄胚挽救技术的研究现状与展望. 园艺学报, 2013,40(9):1645-1655.
	ZHANG J X, NIU R X. The present situation and prospect of embryo rescue technique research in seedless grape breeding. Acta Horticulturae Sinica, 2013,40(9):1645-1655. (in Chinese)
[28]	李桂荣. 无核葡萄胚胎发育的生理特性和胚挽救育种技术的研究[D]. 杨凌: 西北农林科技大学, 2013.
	LI G R. Studies on the physiological characteristics of seedless grape embryo development and embryo rescue breeding technology[D]. Yangling: Northwest A & F University, 2013. (in Chinese)
[29]	李志瑛. 无核葡萄胚挽救育种研究[D]. 杨凌: 西北农林科技大学, 2018.
	LI Z Y. Study on breeding for seedless grape varieties using embryo rescue[D]. Yangling: Northwest A & F University, 2018. (in Chinese)
[30]	徐可遥. 利用胚挽救技术选育香味无核葡萄新种质[D]. 杨凌: 西北农林科技大学, 2018.
	XU K Y. Development of novel muscat seedless grapevine germplasm by embryo rescue[D]. Yangling: Northwest A & F University, 2016. (in Chinese)
[31]	赵雅楠, 骆强伟, 王跃进. 利用胚挽救技术创制无核抗寒葡萄新种质. 中国农业科学, 2018,51(21):4119-4130. doi: 10.3864/j.issn.0578-1752.2018.21.010
	ZHAO Y N, LUO Q W, WANG Y J. Breeding for grape germplasm involved in seedlessness with cold-resistant using embryo rescue. Scientia Agricultura Sinica, 2018,51(21):4119-4130. (in Chinese) doi: 10.3864/j.issn.0578-1752.2018.21.010
[32]	李志瑛, 骆强伟, 王跃进. 无核葡萄胚挽救育种与杂种后代分子标记辅助选择. 果树学报, 2019,36(1):31-42.
	LI Z Y, LUO Q W, WANG Y J. Breeding seedless grapevine via embryo rescue and marker-assisted selection in hybrid progenies. Journal of Fruit Science, 2019, 36(1):31-42. (in Chinese)
[33]	邓向阳, 卫志明. 幼胚长度、2,4-D浓度、光强度等对花生体细胞胚发生的影响及高效再生系统的建立. 植物生理学报, 2000,26(6):525-531.
	DENG X Y, WEI Z M. Influence of length of immature embryos excised for culture, con-centration of 2,4-D, light intensity and other factors on somatic embryogenesis and establishment of a highly efficient system for regeneration of peanut plantlets. Acta Phytophysiologica Sinica, 2000,26(6):525-531. (in Chinese)
[34]	王跃进, LAMIKANRA O, 卢江, RAMMING D. 葡萄无核基因的RAPD遗传标记. 西北农业大学学报, 1996(5):12-21.
	WANG Y J, LAMIKANRA O, LU J, RAMMING D. Analysis of sequencing the RAPD marker linked to seedless genes in grapes. Acta Universitatis Agriculturalis Boreali-Occidentalis, 1996(5):12-21. (in Chinese)
[35]	LAHOGUE F, THIS P, BOUQUET A. Identification of a codominant scar marker linked to the seedlessness character in grapevine. Theoretical and Applied Genetics, 1998,97(5/6):950-959. doi: 10.1007/s001220050976
[36]	MEJIA N, GEBAUER M, MUNOZ L, HEWSTONE N, MUNOZ SCHICK C E, HINRICHSEN P. Identification of QTLs for seedlessness, berry size, and ripening date in a seedless × seedless table grape progeny. American Journal of Enology & Viticulture, 2007,58(4):499-507.
[37]	MEJIA N, HINRICHSEN P. A new, highly assertive scar marker potentially useful to assist selection for seedlessness in table grape breeding. Acta Horticulturae, 2003,603:559-564.

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化合物 Compound		沙巴珍珠 Pearl of Csaba	早玫瑰 Zao Meiguixiang	玫瑰香 Muscat Hamburg	亚历山大 Muscat of Alexandria	巨玫瑰 Jumeigui	金星无核 Venus Seedless	意大利 Italia	粉红玫瑰 Muscat Rose	阳光玫瑰 Shine- Muscat	无核白 Thompson Seedless
醛类Aldehydes	总醛 Total aldehydes	8.9300	4.4924	9.2314	4.9050	15.8080	15.7184	8.0026	10.1754	2.1530	15.5756
醇类 Alcohols	总醇 Total alcohols	2.0900	0.0842	0.2296	1.4376	1.4816	0.3200	0.1754	0.5486	0.5210	0.1138
烯类 Olefins	总烯 Total olefins	0.3522	0.0504	0.0612	0.2074	0.1184	0.1266	0	0.0872	0.0816	0
烷类 Alkenes	总烷 Total alkenes	0.0362	0.0328	0.0098	0.0112	1.8162	0.2784	0.0154	0.0314	0.0588	0.3464
酮类 Ketones	总酮 Total ketones	0	0	0.0848	0.0076	0	0.0234	0.0382	0.0122	0	0
酯类 Esters	总酯 Total esters	0	0	0	0.1202	0.6918	0.0084	0	0.0122	0.0094	0
酸类 Acids	总酸 Total acids	0	0	0	0.0328	0	0.0116	0.0272	0	0	0
萜烯类 Terpenes	香茅醇 Citronellol	0.2912	0.0062	0	0	0.3490	0	0	0	0	0
	香叶醇 Geraniol	0.4432	0.0152	0.0088	0.1524	0.6448	0	0.0246	0.0446	0	0
	香叶醛 Citral	0.0608	0.0024		0.0286	0.0244	0	0	0	0	0
	里那醇 Linalool	0.5532	0.0396	0.1656	1.0790	0.0980	0	0	0.1184	0.4812
	松油醇 Terpineol	0.0340	0	0	0	0	0	0	0	0	0
	à-松油醇 à-Terpineolα-	0	0	0		0.0102	0	0	0.0156	0	0
	脱氢芳樟醇 1,5,7-Octatrien-3-ol, 3,7- dimethyl	0	0	0	0.0035	0	0	0	0	0	0
	总萜烯Total terpenes	1.3824	0.0634	0.1744	1.2636	1.1264	0	0.0246	0.1788	0.4812	0
其他 Others		0.0234	0.0274	0.0374	0.0454	0.2674	0.0516	0.0554	0.1038	0.0076	0.0194
总挥发性物质含量 Total volatile matter content		12.8142	4.7506	9.8286	8.0308	21.3098	16.5384	8.3388	11.1496	3.3126	16.0552

杂交组合 Cross	果粒数 Number of berries	胚珠数 Number of cultured ovule	发育胚Developed embryos		成苗Plantlets formation
杂交组合 Cross	果粒数 Number of berries	胚珠数 Number of cultured ovule	数量 Number	占比 Rate (%)	数量 Number	占比 Rate (%)
爱神玫瑰×玫瑰香 Aishenmeigui × Muscat Hamburg	285	508	75	15.69	14	18.67
爱神玫瑰×阳光玫瑰 Aishenmeigui × Shine-Muscat	1504	2086	335	16.06	79	23.58
爱神玫瑰×昆香无核 Aishenmeigui × Kunxiang Seedless	620	750	71	9.47	17	23.49
火焰无核×红亚历山大 Flame Seedless × Red Alexander	925	1208	133	11.01	50	37.59
火焰无核×阳光玫瑰 Flame Seedless × Shine-Muscat	638	922	98	10.80	20	20.41
火焰无核×新郁 Flame Seedless × Xinyu	665	635	34	5.48	8	23.53
昆香无核×爱神玫瑰 Kunxiang Seedless × Aishenmeigui	2050	2184	305	13.97	70	22.95
昆香无核×玫瑰香 Kunxiang Seedless × Muscat Hamburg	385	405	102	25.19	0	0
克瑞森无核×玫瑰香 Crimson Seedless × Muscat Hamburg	435	441	19	4.31	1	5.26
克瑞森无核×新郁 Crimson Seedless × Xinyu	635	540	16	5.61	1	6.25
红宝石无核×玫瑰香 Ruby Seedless × Muscat Hamburg	1160	1895	395	20.84	186	47.09
红宝石无核×红亚历山大 Ruby Seedless × Red Alexander	1308	2956	763	25.81	249	32.63
红宝石无核×爱神玫瑰 Ruby Seedless × Aishenmeigui	1243	2486	1139	48.59	589	51.71
合计 Total	11853	17016	3485	20.48	1284	36.84

杂交组合 Cross	处理组 Treatment	生长调节剂成分 Exogenous hormone (mg·L^-1)				胚珠数 Number of cultured ovules	发育胚 Developed embryos		萌发 Germination
杂交组合 Cross	处理组 Treatment	2,4-D	KT	ZT	NAA	胚珠数 Number of cultured ovules	数量Number	占比 Rate (%)	数量Number	占比 Rate (%)
昆香无核×爱神玫瑰 Kunxiang Seedless × Aishenmeigui	T1	0	0	0	0	135	22	16.30b	10	7.41a
	T2	0	1	1	0.5	150	33	22.00a	17	11.33a
	T3	0	2	2	1	150	26	17.33b	14	9.33a
	T4	1	0	1	0.5	150	7	4.67bc	1	0.67b
	T5	1	1	2	0	132	7	5.30b	2	1.52b
	T6	1	2	0	0.5	140	1	0.72c	1	0.72b
	T7	2	0	2	0.5	150	10	6.67b	2	1.33b
	T8	2	1	0	1	150	4	2.67c	1	0.67b
	T9	2	2	1	0	150	6	4.00bc	1	0.67b

杂交组合 Cross	胚类型 Embryo code	接种胚数Cultured embryos		发育胚Developed embryos		成苗Plantlets formation
杂交组合 Cross	胚类型 Embryo code	数量 Number	占比 Rate (%)	数量 Number	占比 Rate (%)	数量Number	占比 Rate (%)
昆香无核×爱神玫瑰 Kunxiang Seedless × Aishenmeigui	G	55	47.41	23	41.82	12	21.82
	H	26	22.41	22	84.62	6	23.08
	F	29	25.00	26	89.66	14	48.28
	C	2	1.72	2	100.00	2	100.00
	A	4	3.45	4	100.00	1	25.00
红宝石无核×玫瑰香 Ruby Seedless × Muscat Hamburg	G	77	39.49	32	41.56	17	22.08
	H	5	2.56	2	40.00	1	20.00
	F	106	54.36	96	90.57	62	58.49
	C	4	2.05	3	75.00	3	75.00
	A	3	1.54	1	33.33	0	0

Optimization of Embryo Rescue Technique and Production of Potential Seedless Grape Germplasm with Rosy Aroma

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