棉花内生蜡状芽孢杆菌YUPP-10对棉花黄萎病的防治作用及机制

doi:10.3864/j.issn.0578-1752.2017.14.008

Abstract

Abstract: 【Objective】Control of Verticillium wilt is a worldwide problem in agriculture and horticulture, screening of effective biocontrol microbial resources has become one of the important ways to control Verticillium wilt. The use of microbial antagonists to control these pathologies fits modern sustainable agriculture criteria. The objective of this study is to screen an efficient antagonistic bacteria, and characterize the biocontrol mechanism of bacteria against Verticillium wilt, thus providing a technical basis for control of Verticillium wilt of cotton with biocontrol bacteria. 【Method】An endophytic bacterium, which can hydrolyze polysaccharides with β-1,4 linkage, was isolated by enrichment medium, which was made of konjac glucomannan as carbon source. The inhibition and prevention of YUPP-10 against V. dahliae and Verticillium wilt were tested. The inhibition rate of YUPP-10 against V. dahliae was assessed using the confront culture method, enclosed chamber test and hanging drop method, cultured microsclerotia with aseptic culture filtrate, and the control effect of cottonon Verticillium wilt was detected by substrate inoculation method in a pot experiment. Induced disease resistance of YUPP-10 in cotton were analysed by embryo and leaves inoculated with V. dahliae, sedimentation of lignin and reactive oxygen species (ROS). In addition, the expression level of defense genes in G. hirsutum leaves were detected by real-time quantitative PCR (qRT-PCR). 【Result】A Bacillus cereus isolate YUPP-10 was screened. The results showed that YUPP-10 and its volatile organic compounds significantly inhibited the colony growth of V. dahliae, with the width of the inhibition zone of YUPP-10 was 0.73 cm after 7 days, and the inhibition rate of volatile organic compounds of YUPP-10 was 77.03% after 10 days. B. cereus YUPP-10 culture filtrate (CF) suppressed V. dahliae spore and microsclerotia germination in a dose-dependent manner. The inhibition rate of CF on V. dahliae spore and microsclerotia germination ranged from 17.22% to 71.25% and 10.69% to 26.62%, respectively. The inhibition rate was 80.60%, when cotton seedlings were pro-inoculated with substrate containing YUPP-10 in a pot experiment. YUPP-10 induced disease resistance, including immature embryo and leaves against V. dahliae, ROS, sedimentation of lignin and some defense genes, such as PAL, POD, PPO, CHI and PR10.【Conclusion】It was concluded that YUPP-10 is an efficient biocontrol agent that protects cotton plant from V. dahliae infection. Our data demonstrate that V. dahliae growth is restricted and the additional signals from YUPP-10 must participate in the regulation of the immune response against V. dahliae. Therefore, the YUPP-10 has a great potential in controlling Verticillium wilt.

Key words: endophytic bacterium, Verticillium wilt, induced resistance, defense mechanism

ZHOU JingLong, FENG ZiLi, FENG HongJie, LI YunQing, YUAN Yuan, LI ZhiFang, WEI Feng, SHI YongQiang, ZHAO LiHong, SUN ZhengXiang, ZHU HeQin, ZHOU Yi. Biocontrol Effect and Mechanism of Cotton Endophytic Bacterium Bacillus cereus YUPP-10 Against Verticillium Wilt in Gossypium hirsutum[J].Scientia Agricultura Sinica, 2017, 50(14): 2717-2727.

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