Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (2): 361-370.doi: 10.3864/j.issn.0578-1752.2016.02.016

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Correlation of the Relative Levels of Insulin-Like Growth Factor - I and Myostatin mRNA Expression and Asynchronous Development of Skeletal Muscles Development in Ducks During Early Development

HU Yan, LIU Hong-xiang, SHAN Yan-ju, JI Gai-ge, SHU Jing-ting, XU Wen-juan, ZHU Chun-hong, TAO Zhi-yun, LI Hui-fang   

  1. Jiangsu Institute of Poultry Science/Jiangsu Provincial Key Laboratory of Poultry Heredity & Breeding, Yangzhou 225003, Jiangsu
  • Received:2014-09-03 Online:2016-01-16 Published:2016-01-16

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Abstract: 【Objective】 The present experiment was conducted to study the mRNA expression characterization of insulin-like growth factor 1 (IGF-I) and myostatin A (MSTN-A), thedevelopmental pattern in duck skeletal muscles and their correlation during embryogenesis and post-hatching development in different breeds of ducks. 【Method】We have compared the ontogeny of body weight, the weight of pectoralis major (PM) and lateral gastrocnemius muscle (LM) and the mRNA expression of insulin-like growth factor-I (IGF-I) and myostatin A (MSTN-A) at the embryonic days 13, 17, 21, 25, 27 and the neonatal age of 7 days of Gaoyou duck and Jinding duck. 【Result】During embryogenesis and post-hatching development, the weight of PM and LM showed an extreme significant difference by duck variety and time. The mRNA expression of IGF-I and MSTN-A in duck breast/leg muscles had significantly negative correlations with body weight and weight of skeletal muscle, but had a positive correlation with breast/leg muscles index. During early development, there were extremely significant positive correlations between the mRNA expression of IGF-I and MSTN-A, and there were peaks in the mRNA expression of IGF-I and MSTN-A at embryonic age of 13 days. The turning points of IGF-I mRNA expression in duck PM were matched with PM growth, and but not for LM. During duck myogenesis, the profile of MSTN-A expression showed synchronization with growth of skeletal muscle and the peaks of proliferation in changes of myofiber characters, and the differences of the IGF-I/MSTN-A mRNA ratio in PM between the two myoblasts. The IGF-I/MSTN-A mRNA ratio in PM and LM were significantly higher with a similar trend in the breeds matched the timing of varieties difference in PM weight. 【Conclusion】 During embryogenesis and post-hatching development, the duck PM and LM developed in an asynchronous pattern. The relative levels of IGF-I and MSTN mRNA may participate to set muscle growth rate along development.

Key words: duck, early development, skeletal muscle, IGF-I, MSTN-A

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