光照强度对樱桃谷肉鸭c-fos、生物钟基因表达 及褪黑激素的影响

doi:10.3864/j.issn.0578-1752.2020.04.016

Abstract

Abstract:

【Objective】 The objective of this study was to investigate the effects of different intensities of light stimulation on c-fos and related clock genes in hypothalamus, diencephalon, pituitary and mesencephalon, as well as melatonin levels in plasma and liver of cherry valley meat duck under continuous darkness. 【Method】 A total of one hundred and forty four 1-day-old cherry valley meat ducks were raised to the age of 21 days. 22-day-old cherry valley meat ducks were randomly divided into 2 groups. Each group had 6 replicates, with 12 ducks per replicate, and kept dark for 7 days, the ducks were given ad libitum access to water and feed. After 7 days lasting darkness, 28-day-old ducks received a LED white light stimulation of different intensities for 1.5 h and then a dark adaptation for 1.5 h. One duck from each replicate was slaughted for sample collection, the expression levels of c-fos and circadian clock genes in the hypothalamus, diencephalon, pituitary and mesencephalon were measured, and plasma and liver melatonin levels were measured. 【Result】 The expression level of c-fos in hypothalamus and mesencephalon of the 10 Lx group was significantly higher than that in the 80 Lx group (P＜0.05). The level of c-fos in the diencephalon was significantly lower than that in the 80 Lx group (P＜0.05), and there was no significant difference of c-fos expression in pituitary in the two groups(P＞0.05). Compared with the 80 Lx group, the expression of clock gene clock, Bmal1, Bmal2 and Per2 in the 10 Lx group in the hypothalamus was significantly increased (P＜0.05), and the expression of Cry1 was little increased in the 10 Lx group (P=0.06) .The expression level of Bmal1 in the diencephalon was significantly higher in the 10 Lx group (P＜0.05), while Bmal2, Clock, Per2 and Cry1 showed no significant difference (P＞0.05) in two groups. The expression of Clock and Per2 in the mesencephalon was significantly higher in the 10 Lx group than that in the 80 Lx group (P＜0.05). The expression of Bmal1, Bmal2 and Cry1 in the two groups had no significant difference (P＞0.05). The expression of clock genes in pituitary was not significant affect by light intensity (P＞0.05). Compared with the 80 Lx, the plasma level of melatonin in 10 Lx group was significantly lower (P＜0.05), while the melatonin levels in the liver were significantly higher. 【Conclusion】 In the continuous darkness, transient high intensity light stimulation could inhibit the expression of c-fos in the hypothalamus and mesencephalon, and increase the expression of c-fos in the diencephalon, while the stimulation could attenuate the amplitude of gene expression in the hypothalamus and diencephalon of the meat duck.

Key words: cherry valley meat duck, continuous darkness, light intensity, c-fos, biological clock

JiaJie CUI,Qiang XIE,ShuangShuang ZHAI,Tao GONG,YongWen ZHU,Lin YANG,WenCe WANG. Effects of Light Intensity on c-fos, Biological Clock Gene Expression and Melatonin in Cherry Valley Meat Ducks[J].Scientia Agricultura Sinica, 2020, 53(4): 848-856.

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References 44

[1]	DEEP A, RAGINSKI C, SCHWEAN-LARDNER K, FANCHER B I, CLASSEN H L . Minimum light intensity threshold to prevent negative effects on broiler production and welfare. British Poultry Science, 2013,54(6):686-694.
[2]	JIANG N, WANG Z, CAO J, DONG Y, CHEN Y . Role of monochromatic light on daily variation of clock gene expression in the pineal gland of chick. Journal of Photochemistry and Photobiology B: Biology, 2016, (164):57-64.
[3]	DAWSON A, KING V M, BENTLEY G E, BALL G F . Photoperiodic control of seasonality in birds. Journal of Biological Rhythms, 2001,16(4):365-380.
[4]	EDGAR R S, GREEN E W, YUWEI Z, GERBEN V O, MARIA O, XIMING Q, YAO X, MIN P, VALEKUNJA U K, FEENEY K A . Peroxiredoxins are conserved markers of circadian rhythms. Nature, 2012,485(7399):301-312.
[5]	BUHR E D, TAKAHASHI J S . Molecular components of the mammalian circadian clock. Handbook of Experimental Pharmacology, 2013(217):3-27.
[6]	石雷, 李云雷, 孙研研, 陈继兰 . 光照节律调控鸡繁殖性能机制研究进展. 中国农业科学, 2018,51(16):3191-3200.
	SHI L, LI Y L, SUN Y Y, CHEN J L . Research progress on the regulatory mechanism of lighting schedule affecting the reproduction performance of chickens. Scientia Agricultura Sinica, 2018, 51(16):3191-3200. (in Chinese)
[7]	GWINNER E, BRANDST TTER R . Complex bird clocks. Philosophical Transactions of the Royal Society of London, 2001,356(1415):1801-1810.
[8]	王晓鹃, 刘磊, 焦洪超, 赵景鹏, 林海 . 生物钟在蛋鸡排卵-产蛋过程中的调控作用. 中国农业科学, 2018,51(16):3181-3190.
	WANG X J, LIU L, JIAO H C, ZHAO J P, LIN H . Regulation of biological clock in ovulation-laying of laying hens. Scientia Agricultura Sinica, 2018,51(16):3181-3190. (in Chinese)
[9]	CASSONE V M . Avian circadian organization: A chorus of clocks. Frontiers in Neuroendocrinology, 2014,35(1):76-88.
[10]	TURKOWSKA E, PIETRUSZKA D, SKWARLO-SONTA K . Thymic E4bp4 gene transcription is up-regulated in the chicken during experimental peritonitis modified by the season-related lighting conditions. Developmental & Comparative Immunology, 2016,65:309-313.
[11]	HONDA K, KONDO M, HIRAMOTO D, SANEYASU T, KAMISOYAMA H . Effects of continuous white light and 12 h white-12 h blue light-cycles on the expression of clock genes in diencephalon, liver, and skeletal muscle in chicks. Comparative Biochemistry & Physiology Part a Molecular & Integrative Physiology, 2017,207:73-78.
[12]	YOSHIMURA T, SUZUKI Y, MAKINO E, SUZUKI T, KUROIWA A, MATSUDA Y, NAMIKAWA T, EBIHARA S . Molecular analysis of avian circadian clock genes. Molecular Brain Research, 2000,78(1):207-215.
[13]	JIANG N, WANG Z, CAO J, DONG Y, CHEN Y . Effect of monochromatic light on circadian rhythmic expression of clock genes in the hypothalamus of chick. Journal of Photochemistry & Photobiology B Biology, 2017,173:476.
[14]	LUKSCH H, COX K, KARTEN H J . Bottlebrush dendritic endings and large dendritic fields: motion-detecting neurons in the tectofugal pathway. Journal of Comparative Neurology, 2015,423(2):243-260.
[15]	ARONIN N, SAGAR S M, SHARP F R, SCHWARTZ WJ . Light regulates expression of a Fos-related protein in rat suprachiasmatic nuclei. Proceedings of the National Academy of Sciences of the United States of America, 1990,87(15):5959-5962.
[16]	GINTY D D, KORNHAUSER J M, THOMPSON M A, BADING H, MAYO K E, TAKAHASHI J S, GREENBERG M E . Regulation of CREB phosphorylation in the suprachiasmatic nucleus by light and a circadian clock. Science, 1993,260(5105):238-241.
[17]	REA M A . Different populations of cells in the suprachiasmatic nuclei express c- fos in association with light-induced phase delays and advances of the free-running activity rhythm in hamsters. Brain Research, 1992,579(1):107-112.
[18]	PORTER L, PORTER A, POTTER H, ALENCIKS E, FRALEY S M, FRALEY G S . Low light intensity in Pekin duck breeder barns has a greater impact on the fertility of drakes than hens. Poultry Science, 2018, 2018-12-01, 97(12):4262-4271.
[19]	HUNT S P, PINI A, EVAN G . Induction of c-fos protein in spinal cord neurones following sensory stimulation. Nature, 1987,328(6131):632-634.
[20]	兰晓宇, 胡满, 程金金, 张毅, 胥佳利 . 不同强度光照对母鸡中脑和间脑c-fos基因表达的影响. 中国农学通报, 2010,26(3):11-14.
	LAN X Y, HU M, CHENG J J, ZHANG Y, XU J L . Influence on the expression of c-fos gene by different intensity light stimulation in hen mesencephalon and diencephalon. Chinese Agricultural Science Bulletin, 2010,26(3):11-14. (in Chinese)
[21]	程金金, 胡满, 张毅, 郭洁 . 光诱导c-fos基因在不同日龄母鸡中脑和间脑的表达. 河北农业大学学报, 2009,32(3):85-88.
	CHENG J J, HU M, ZHANG Y, GUO J . Expression of c-fos gene induced by light stimulation in hen mesencephalon and diencephalons at different day -age. Journal of Agricultural University of Hebei, 2009,32(3):85-88. (in Chinese)
[22]	DEEP A, RAGINSKI C, SCHWEAN-LARDNER K, FANCHER B I, CLASSEN H L . Minimum light intensity threshold to prevent negative effects on broiler production and welfare. British Poultry Science, 2013,54(6):686-694.
[23]	OELSCHL GER H H, NAKAMURA M, HERZOG M, BURDA H . Visual system labeled by c-Fos immunohistochemistry after light exposure in the 'blind' subterranean Zambian mole-rat (Cryptomys anselli). Brain Behavior & Evolution, 2000,55(4):209-220.
[24]	THAYANANUPHAT A, KANG ST, MILLAM J, EL-HALAWANI M . Rhythm-dependent light induction of the c-fos gene in the turkey hypothalamus. Journal of Neuroendocrinology, 2010,19(6):407-417.
[25]	OOSTHUIZEN M K, BENNETT N C, COOPER H M . Photic induction of Fos in the suprachiasmatic nucleus of African mole-rats: Responses to increasing irradiance. Chronobiology International, 2010,27(8):1532-1545.
[26]	EARNEST D J, IADAROLA M, YEH H H, OLSCHOWKA J A . Photic regulation of c- fos expression in neural components governing the entrainment of circadian rhythms. Experimental Neurology, 1990,109(3):353-361.
[27]	HONRADO G I, JOHNSON R S, GOLOMBEK D A, SPIEGELMAN B M, PAPAIOANNOU V E, RALPH M R . The circadian system of c- fos deficient mice. Journal of Comparative Physiology A, 1996,178(4):563-570.
[28]	WOLLNIK F, BRYSCH W, UHLMANN E, GILLARDON F, BRAVO R, ZIMMERMANN M, SCHLINGENSIEPEN KH, HERDEGEN T . Block of c-fos and junb expression by antisense oligonucleotides inhibits light-induced-phase shifts of the mammalian circadian clock. European Journal of Neuroscience, 2010,7(3):388-393.
[29]	YASUO S, EBIHARA S, YOSHIMURA T . Circadian expression of clock gene in the optic tectum of Japanese quail. Brain Research, 2004,1005(1):193-196.
[30]	SHUBONI D, YAN L . Nighttime dim light exposure alters the responses of the circadian system. Neuroscience, 2010,170(4):1172-1178.
[31]	GRONE B P, CHANG D, BOURGIN P, CAO V, FERNALD R D, HELLER H C, RUBY N F . Acute light exposure suppresses circadian rhythms in clock gene expression. Journal of Biological Rhythms, 2011,26(1):78.
[32]	HIEKE A S C, HUBERT S M, ATHREY G . Circadian disruption and divergent microbiota acquisition under extended photoperiod regimens in chicken. 2018.
[33]	MA S, WANG Z, CAO J, DONG Y, CHEN Y . Effect of Monochromatic Light on Circadian Rhythm of Clock Genes in Chick Pinealocytes. Photochemistry & Photobiology, 2018.
[34]	DE J M, JENINGA L, OUYANG J Q, VAN O K, SPOELSTRA K, VISSER M E . Dose-dependent responses of avian daily rhythms to artificial light at night. Physiology & Behavior, 2016,155(3):172-179.
[35]	PANDI-PERUMAL S R, SRINIVASAN V, MAESTRONI G, CARDINALI D P, POEGGELER B, HARDELAND R . Melatonin - Nature's most versatile biological signal? FEBS Journal, 2010,273(13):2813-2838.
[36]	CHONG N W, BERNARD M, KLEIN D C . Characterization of the chicken serotonin N-acetyltransferase gene. Journal of Biological Chemistry, 2000,275(42):32991-32998.
[37]	LARSEN P J . Tracing autonomic innervation of the rat pineal gland using viral transneuronal tracing. Microscopy Research & Technique, 1999,46(4‐5):296-304.
[38]	T N, T Y . Molecular basis for regulating seasonal reproduction in vertebrates. Journal of Endocrinology, 2016,229(3):R117.
[39]	华登科, 贺海军, 贾亚雄, 李冬立, 唐诗, 白皓, 刘念, 秦宁, 孙研研, 陈余 . 光照强度对北京油鸡生长激素和褪黑激素含量的影响. 中国家禽, 2014,36(19):30-32.
	HUA D K, HE H J, JIA Y X, LI L D, TANG S, BAI H, LIU N, QIN N, SUN Y Y, CHEN Y . Effect of light intensity on growth hormone and melatonin secretion of Beijing You Chicken. China Poultry, 2014,36(19):30-32. (in Chinese)
[40]	LYNCH H J, HO M, WURTMAN R J . The adrenal medulla may mediate the increase in pineal melatonin synthesis induced by stress, but not that caused by exposure to darkness. Journal of Neural Transmission, 1977,40(2):87.
[41]	ALEXIES D S, ORELLANA J A, CARLOS CF, JUAN M, GABRIELA DV, MARÍA S F, URSULA W, CONCHA M L, FRANCISCO A, . Chronic stress decreases the expression of sympathetic markers in the pineal gland and increases plasma melatonin concentration in rats. Journal of Neurochemistry, 2010,97(5):1279-1287.
[42]	JACOBO W . Role of epinephrine in acute stress. Endocrinology & Metabolism Clinics of North America, 2002,31(1):79-106.
[43]	WANG Y, ZHANG Z, HONGWEI, GUOPENG, LI Y, LI J, XI L, LIU Q . Stimulation role of epinephrine in transcription of the melatonin synthesis key enzyme AANAT in the pineal gland of broilers. Molecular and Cellular Biochemistry, 2019,453(1):111-119.
[44]	TORDJMAN S, CHOKRON S, DELORME R, CHARRIER A, BELLISSANT E, JAAFARI N, FOUGEROU C . Melatonin: pharmacology, functions and therapeutic benefits. Current Neuropharmacology, 2017,15(3):434-443.

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基因 Gene	序列号 Accession No.	引物序列（5'-3'） Primer sequence (5′-3′)	大小 Product (bp)
c-fos	NM_205508.1	F: CAGCCCACCCTCATCTCCTC	144
c-fos	NM_205508.1	R: CCTTCGTCCGATGCTCTGC	144
Clock	XM_005012718.3	F: GCACTCCTCCAAGGCAAAG	169
Clock	XM_005012718.3	R: AACACTGGCTGGGACACG	169
Cry1	XM_005011518.2	F: GGCTGTTTGTCTTGTCGT	145
Cry1	XM_005011518.2	R: ACCGTGGATTGTTAGTCG	145
Bmal1	XM_013106919.2	F: TGTTTCCACCAGCGTTCT	133
Bmal1	XM_013106919.2	R: AATGCCAGGGACAGTAGG	133
Bmal2	NM-204133.1	F: CGGCGTTCCTTCTTCTGTC	165
Bmal2	NM-204133.1	R: TTCCTCTTCCACTCCCACC	165
Per2	XM_005028592.3	F: AAGCGTTGTATCACTCAC	106
Per2	XM_005028592.3	R: ACTCGGACCATCTTCTAT	106
β-actin	NM_001310421.1	F: TACGCCAACACGGTGCTG	215
β-actin	NM_001310421.1	R: GATTCATCATACTCCTGCTTG	215

	10 Lx	80 Lx	标准误 Sem	P值 P value
下丘脑Hypothalamus	1.15A	0.87B	0.05	＜0.01
间脑Diencephalon	1.43A	0.76B	0.08	＜0.01
垂体Pituitary	1.20	1.04	0.19	0.60
中脑Mesencephalon	1.26	1.20	0.20	0.87

	10 Lx	80 Lx	标准误 Sem	P值 P value
下丘脑Hypothalamus	1.03A	0.51B	0.09	＜0.01
间脑Diencephalon	1.03	0.72	0.12	0.08
垂体Pituitary	1.05	0.98	0.12	0.74
中脑Mesencephalon	0.89	0.76	0.12	0.45

	10 Lx	80 Lx	标准误 Sem	P值 P value
下丘脑Hypothalamus	0.96a	0.44b	0.13	0.02
间脑Diencephalon	1.09	0.65	0.35	0.19
垂体Pituitary	0.78	0.54	0.10	0.14
中脑Mesencephalon	0.81a	0.50b	0.07	0.02

	10 Lx	80 Lx	标准误 Sem	P值 P value
下丘脑Hypothalamus	0.93	0.48	0.14	0.06
间脑Diencephalon	0.90	0.62	0.10	0.09
垂体Pituitary	1.00	0.76	0.12	0.23
中脑Mesencephalon	1.01	0.86	0.08	0.18

Effects of Light Intensity on c-fos, Biological Clock Gene Expression and Melatonin in Cherry Valley Meat Ducks

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	10 Lx	80 Lx	标准误 Sem	P值 P Value
下丘脑Hypothalamus	0.84A	0.41B	0.05	＜0.01
间脑Diencephalon	0.93	0.78	0.36	0.11
垂体Pituitary	1.08	0.97	0.09	0.43
中脑Mesencephalon	0.82a	0.59b	0.06	0.04

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