水稻产量性状竞争优势QTL定位

doi:10.3864/j.issn.0578-1752.2016.02.004

Abstract

Abstract: 【Objective】 This study was conducted to determine quantitative trait loci (QTLs) associated with the standard heterosis of yield traits and to analyze the genetic basis of standard heterosis in rice. 【Method】 A recombinant inbred line (RIL) population was derived from crosses between rice restorer lines Teqing and six IRBB lines that are near isogenic lines in the genetic background of IR24. A testcross population was also constructed by crossing the 204 RILs to male sterile line Zhong 9A. The 204 sets of RILs and corresponding F₁ were grown side by side for two years in the same trial site. They were planted in two replications. At maturity, the middle four plants of each replication for each line were harvested together. The number of panicles per plant (NP), number of grains per panicle (NGP), number of spikelets per panicle (NSP), spikelet fertility (SF), 1000-grain weight (TGW) and grain yield per plant (GY) were measured. Mean values over the two replications were used for data analysis. A data set derived by subtracting the trait value of a RIL from that of its corresponding F₁ was used for QTL analysis using QTLNetwork 2.0. A putative QTL was claimed using a genome-wise type I error of P<0.05 determined by 1000 permutations. 【Result】 Significant positive correlations between the RILs and F₁s were observed for all the six traits. The correlation coefficients were highest for TGW (0.903), lowest for NP (0.333) and GY (0.357), and intermediate for SF (0.406), NGP (0.448) and NSP (0.680). A total of 16 QTLs distributed on chromosomes 2, 3, 5, 6, 8 and 10 were detected, including three (3) for NGP, four (4) for NSP, three (3) for SF, four (4) for TGW, and two (2) for GY. The phenotypic variance explained by a single QTL ranged from 1.7 % to 22.1 %. All three QTLs affecting NGP showed higher standard heterosis for Zhong 9A/IR24 than Zhong 9A/Teqing. For NSP, SF and TGW, three, two and two QTLs had higher standard heterosis for Zhong 9A/IR24 than Zhong 9A/Teqing, and one, one and two QTLs were higher in standard heterosis for Zhong 9A/Teqing than Zhong 9A/IR24. Of the two QTLs associated with GY, qGY2 overlapped with qNGP2 for NGP and qNSP2 for NSP, and qGY10 overlapped with qNGP10 for NGP and qSF10 for SF. All six QTLs showed higher standard heterosis for Zhong 9A/IR24 than Zhong 9A/Teqing. 【Conclusion】 F₁ performance was correlated with both parental performance and F₁ heterosis. QTLs for standard heterosis play an important role in the genetic control of F₁ performance of yield traits in rice.

Key words: rice, yield trait, standard heterosis, quantitative trait locus

ZHU Yu-jun, CHEN Jun-yu, ZHANG Zhen-hua, ZHANG Hong-wei, FAN Ye-yang, ZHUANG Jie-yun. QTL Mapping for Standard Heterosis of Yield Traits in Rice[J].Scientia Agricultura Sinica, 2016, 49(2): 232-238.

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QTL Mapping for Standard Heterosis of Yield Traits in Rice

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