氮利用效率差异水稻品种的根系铵离子吸收特性

doi:10.3864/j.issn.0578-1752.2015.22.004

Abstract

Abstract: 【Objective】Two rice varieties were exposed to different levels of external ammonium ion (NH₄⁺) condition to examine the responses and its differences. The research will further reveal the mechanisms of nitrogen use efficiency (NUE) in different absorption properties by analyzing different performances and absorption characteristics.【Method】Two rice varieties of QLSM (High-NUE) and HK3 (Low-NUE) were cultivated hydroponically, and then treated with 3 NH₄⁺concentrations (Low, Middle, High) for 15 days at tillering stage. Furthermore, the morphological characteristics, nitrogen content, nitrogen accumulation, root activity, root respiration and root transmembrane NH₄ ⁺ fluxes were determined.【Result】The results showed that QLSM grew well under low NH₄⁺ condition and showed no significant difference with its control in plant height and tillers. It showed that the total dry matter weight and root N accumulation increased by 25.9% and 74.2% in QLSM, respectively. Whereas, low NH₄⁺ condition significantly reduced plant height, tillers, total dry matter weight and root N accumulation of HK3 by 10.1%, 26.4%, 19.5% and 2.3%, respectively. Furthermore, compared with CK, N content in roots and shoots of the two varieties decreased under low NH₄⁺ condition. Meanwhile, the root activity of QLSM and HK3 sharply reduced by 76.0% and 78.2%, and the root respiration intensity also significantly decreased by 29.5% and 33.8%. The results suggested that root activity and root respiration intensity did not contribute to NH₄⁺ absorption under low NH₄⁺ condition. Moreover, under the condition of low NH₄⁺, NH₄⁺ influx was more pronounced in QLSM, while HK3 showed NH₄⁺ insensitivity in the root meristem zone.【Conclusion】It is concluded that high NH₄⁺ absorptive capacity under low NH₄ ⁺ is associated with enhanced NH₄⁺ influx in the root meristem zone.

Key words: rice, ammonium ion, root activity, root respiration rate, transmembrane NH4+ fluxes, nitrogen use efficiency

ZHANG Xiao-guo, XU Chun-mei, CHEN Song, JI Cheng-lin, ZHANG Xiu-fu, WANG Dan-yingZHANG Xiao-guo, XU Chun-mei, CHEN Song, JI Cheng-lin, ZHANG Xiu-fu, WANG Dan-ying. Absorption Characteristics of Ammonium Ion of Roots in Different Nitrogen Use Efficiency Rice Varieties[J].Scientia Agricultura Sinica, 2015, 48(22): 4428-4436.

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References

[1] Makino A. Photosynthesis, grain yield, and nitrogen utilization in rice and wheat. Plant Physiology, 2011, 155: 125-129.

[2] Good A G, Shrawat A K, Muench D G. Can less yield more? Is reducing nutrient input into the environment compatible with maintaining crop production? Trends in Plant Science, 2004, 9 (12): 597-605.

[3] Peng S B, Huang J L, Zhong X H, Yang J C, Wang G H, Zou Y B, Zhang F S, Zhu Q S, Buresh R, Witt C. Challenge and opportunity in improving fertilizer-nitrogen use efficiency of irrigated rice in China. Agricultural Sciences in China, 2002, 1(7): 776-785.

[4] Lin Y L, Chao Y Y, Huang W D, Kao C H. Effect of nitrogen deficiency on antioxidant status and Cd toxicity in rice seedlings. Plant Growth Regulation, 2011, 64: 263-273.

[5] 黄亿, 李廷轩, 张锡洲, 戢林, 吴沂珀. 氮高效利用基因型大麦氮素转移及氮形态组分特征. 中国农业科学, 2015, 48(6): 1151-1161.

Huang Y, Li T X, Zhang X Z, Ji L, Wu Y P. Characteristics of nitrogen transportation and fractions in different organs of barley genotype with high nitrogen utilization efficiency. Scientia Agricultura Sinica, 2015, 48(6): 1151-1161. (in Chinese)

[6] Ding C Q, You J, Liu Z H, Rehmani M I A, Wang S H, Li G H, Wang Q S, Ding Y F. Proteomic analysis of low nitrogen stress-responsive proteins in roots of rice. Plant Molecular Biology Reporter, 2011, 29: 618-625.

[7] 程建峰, 戴廷波, 荆奇, 姜东, 潘晓云, 曹卫星. 不同水稻基因型的根系形态生理特性与高效氮素吸收. 土壤学报, 2007, 44(2): 266-272.

Cheng J F, Dai T B, Jing Q, Jiang D, Pan X Y, Cao W X. Root morphological and physiological characteristics in relation to nitrogen absorption efficiency in different rice genotypes. Acta Pedologica Sinica, 2007, 44(2): 266-272. (in Chinese)

[8] 曾建敏, 崔克辉, 黄见良, 贺帆, 彭少兵. 水稻生理生化特性对氮肥的反应及与氮利用效率的关系. 作物学报, 2007, 33(7): 1168-1176.

Zeng J M, Cui K H, Huang J L, He F, Peng S B. Responses of physio-biochemical properties to N-fertilizer application and its relationship with nitrogen use efficiency in rice (Oryza sativa L.). Acta Agronomica Sinica, 2007, 33(7): 1168-1176. (in Chinese)

[9] Shi W M, Xu W F, Li S M, Zhao X Q, Dong G Q. Responses of two rice cultivars differing in seedling-stage nitrogen use efficiency to growth under low-nitrogen conditions. Plant and Soil, 2010, 326: 291-302.

[10] Sun H, Qian Q, Wu K, Luo J, Wang S, Zhang C, Ma Y , Liu Q, Huang X , Yuan Q , Han R , Zhao M, Dong G , Guo L , Zhu X , Gou Z , Wang W, Wu Y , Lin H , Fu X . Heterotrimeric G proteins regulate nitrogen-use efficiency in rice. Nature Genetics, 2014, 46(4): 652-657.

[11] Glass A D M, Britto D T, Kaiser B N, Kinghorn J R, Kronzucker H J, Kumar A, Okamoto M, Rawat S, Siddiqi M Y, Unkles S E, Vidmar J J. The regulation of nitrate and ammonium transport systems in plants. Journal of Experimental Botany, 2002, 53: 855-864.

[12] Lian X, Wang S, Zhang J, Feng Q, Zhang L, Fan D, Li X, Yuan D, Han B, Zhang Q. Expression profiles of 10, 422 genes at early stage of low nitrogen stress in rice assayed using a cDNA microarray. Plant Molecular Biology, 2006, 60: 617-631.

[13] Kant S, Bi Y M, Rothstein S J. Understanding plant response to nitrogen limitation for the improvement of crop nitrogen use efficiency. Journal of Experimental Botany, 2011, 62(4): 1499-1509.

[14] 张亚丽, 樊剑波, 段英华, 王东升, 叶利庭, 沈其荣. 不同基因型水稻氮利用效率的差异及评价. 土壤学报, 2008, 45(2): 267-273.

Zhang Y L, Fan J B, Duan Y H, Wang D S, Ye L T, Shen Q R. Variation of nitrogen use efficiency of rice different in genotype and its evaluation. Acta Pedologica Sinica, 2008, 45(2): 267-273. (in Chinese)

[15] 杨建昌. 水稻根系形态生理与产量、品质形成及养分吸收利用的关系. 中国农业科学, 2011, 44(1): 36-46.

Yang J C. Relationships of rice root morphology and physiology with the formation of grain yield and quality and the nutrient absorption and utilization. Scientia Agricultura Sinica, 2011, 44(1): 36-46. (in Chinese)

[16] 徐富贤, 熊洪, 谢戎, 张林, 朱永川, 郭晓艺, 杨大金, 周兴兵, 刘茂. 水稻氮素利用效率的研究进展及其动向. 植物营养与肥料学报, 2009, 15(5): 1215-1225.

Xu F X, Xiong H, Xie R, Zhang L, Zhu Y C, Guo X Y, Yang D J, Zhou X B, Liu M. Advance of rice fertilizer-nitrogen use efficiency. Plant Nutrition and Fertilizer Science, 2009, 15(5): 1215-1225. (in Chinese)

[17] Liu J, Ma J, He C, Li X, Zhang W, Xu F, Lin Y, Wang L. Inhibition of cadmium ion uptake in rice (Oryza sativa) cells by a wall-bound form of silicon. New Phytologist, 2013, 200: 691-699.

[18] Chen G, Guo S W, Kronzucker H J, Shi W M. Nitrogen use efficiency (NUE) in rice links to NH₄⁺ toxicity and futile NH₄⁺ cycling in roots. Plant Soil, 2013, 369(1-2): 351-363.

[19] 鲁如坤. 土壤农业化学分析方法. 北京: 中国农业出版社, 1999: 152-163.

Lu R K. Soil and Agricultural Chemistry Analysis Method. Beijing: China Agriculture Press, 1999: 152-163. (in Chinese)

[20] 黄益宗, 张文强, 招礼军, 曹慧明. Si对盐胁迫下水稻根系活力、丙二醛和营养元素含量的影响. 生态毒理学报, 2009, 4(6): 860-866.

Huang Y Z, Zhang W Q, Zhao L J, Cao H M. Effects of Si on the index of root activity, MDA content and nutritional elements uptake of rice under salt stress. Asian Journal of Ecotoxicology, 2009, 4(6): 860-866. (in Chinese)

[21] Sun J, Chen S, Dai S, Wang R, Li N, Shen X, Zhou X, Lu C, Zheng X, Hu Z, Zhang Z, Song J, Xu Y. NaCl-induced alternations of cellular and tissue ion fluxes in roots of salt-resistant and salt-sensitive poplar species. Plant Physiology, 2009, 149: 1141-1153.

[22] 戢林, 李廷轩, 张锡洲, 余海英. 水稻氮高效基因型根系分泌物中有机酸和氨基酸的变化特征. 植物营养与肥料学报, 2012, 18(5): 1046-1055.

Ji L, Li T X, Zhang X Z, Yu H Y. Characteristics of organic acid and amino acid in root exudates of rice genotype with high nitrogen efficiency. Plant Nutrition and Fertilizer Science, 2012, 18(5): 1046-1055. (in Chinese)

[23] Liu J, Chen F, Olokhnuud C, Glass A D M, Tong Y, Zhang F, Mi G. Root size and nitrogen-uptake activity in two maize (Zea mays) inbred lines differing in nitrogen-use efficiency. Journal of Plant Nutrition and Soil Science, 2009, 172: 230-236.

[24] Liu Y, Ding Y F, Wang Q S, Meng D X, Wang S H. Effects of nitrogen and 6-benzylaminopurine on rice tiller bud growth and changes in endogenous hormones and nitrogen. Crop Science, 2011, 51: 786-792 .

[25] 王双明. 黑、紫粒小麦对氮素吸收及同化特性的初步研究. 中国土壤与肥料, 2008, (6): 28-30.

Wang S M. Primary study on nitrogen uptake and assimilation character of black, purple grain wheat. Soil and fertilizer in China, 2008, (6): 28-30. (in Chinese)

[26] Fan J B, Zhang Y L, Turner D, Duan Y H, Wang D S, Shen Q R. Root physiological and morphological characteristics of two rice cultivars with different nitrogen-use efficiency. Pedosphere, 2010, 20(4): 446-455.

[27] 樊剑波, 张亚丽, 万小羽, 沈其荣. 水稻根系与氮素吸收利用之研究进展. 中国农学通报, 2007, 23(2): 236-240.

Fan J B, Zhang Y L, Wan X Y, Shen Q R. Progress in research of rice root related to nitrogen uptake and utilization. Chinese Agricultural Science Bulletin, 2007, 23(2): 236-240. (in Chinese)

[28] Kronzucker H J, Siddiqi M Y, Glass A D M, Kirk G J D. Comparative kinetic analysis of ammonium and nitrate acquisition by tropical lowland rice: Implications for rice cultivation and yield potential. New Phytologist, 2000, 145: 471-476.

[29] Wang M Y, Siddiqi M Y, Ruth T J, Glass A D M. Ammonium uptake by rice roots. II. Kinetics of ¹³NH₄⁺ influx across the plasmalemma. Plant Physiology, 1993, 103: 1259-1267.

[30] von Wiren N, Gazzarrini S, Goion A, Frommer W B. The molecular physiology of ammonium uptake and retrieval. Plant Biology, 2000, 3: 254-261.

[31] Britto D T, Kronzucker H J. Futile cycling at the plasma membrane: A hallmark of low-affinity nutrient transport. Trends in Plant Science, 2006, 11: 529-534.

[32] 魏海燕, 张洪程, 马群, 戴其根, 霍中洋, 许轲, 张庆, 黄丽芬. 不同氮肥利用效率水稻基因型剑叶光合特性. 作物学报, 2009, 35(12): 2243-2251.

Wei H Y, Zhang H C, Ma Q, Dai Q G, Huo Z Y, Xu K, Zhang Q, Huang L F. Photosynthetic characteristics of flag leaf in rice genotypes with different nitrogen use efficiencies. Acta Agronomica Sinica, 2009, 35(12): 2243-2251. (in Chinese)

[33] Bi Y M, Kant S, Clark J, Gidda S, Ming F, Xu J Y, Rochon A, Shelp B J, Hao L X, Zhao R, Mullen R T, Zhu T, Rothstein S J. Increased nitrogen-use efficiency in transgenic rice plants over-expressing a nitrogen-responsive early nodulin gene identified from rice expression profiling. Plant, Cell and Environment, 2009, 32: 1749-1760.

[34] Céline M D, Françoise D V, Dechorgnat J, Chardon F, Gaufichon L, Suzuki A. Nitrogen uptake, assimilation and remobilization in plants: Challenges for sustainable and productive agriculture. Annals of Botany, 2010, 105: 1141-1157.

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