干旱和盐胁迫条件下枣树谷胱甘肽过氧化物酶基因（ZjGPX）的差异表达及功能分析

doi:10.3864/j.issn.0578-1752.2015.14.012

Abstract

Abstract: 【Objective】As a foundation for further studying on the application of fruit stress tolerance genetic engineering, this study analyzed the function of a cDNA encoding GPX, which was isolated from the fruit-bearing shoot of Ziziphus jujuba Mill. Hupingzao and designated as ZjGPX (GenBank accession No. AB608053). 【Method】 The ZjGPX sequence was analyzed and expression functions were forecasted by using bioinformatics analysis tool first. The differential expressions of ZjGPX on drought and salt stresses were tested by real-time quantitative PCR, and a vector named PEZR(K)-LNY-ZjGPX was constructed and introduced into Arabidopsis by floral dip method. Transgenic and wild Arabidopsis plants were tested under NaCl and drought stresses to verify ZjGPX’s function.【Result】Sequence analysis indicated that ZjGPX was 510 bp in length, encoding 169 amino acids. Blast analysis exhibited that ZjGPX was highly homologous to other GPXs (>80%). Real-time quantitative PCR analysis showed that ZjGPX expression could be rapidly induced by drought and salt stresses just in 15 minutes in Z. jujuba Mill. Lajiaozao test tube seedling. After agrobacterium-mediated transformation, 10 ZjGPX transgenic Arabidopsis lines were obtained. Results of the drought resistance and salt tolerance tests pointed out that in the stress treatments, the transgenic plants exhibited higher seed germination rates than the untransformed control plants. After 15 days of stress treatment, the untransformed control plants showed signs of death, but the transgenic plants maintained normal growth.【Conclusion】The results showed that ZjGPX was likely related to drought and salt resistances and over-expression of ZjGPX gene increased the drought resistance and salt tolerance of the transgenic Arabidopsis.

Key words: Ziziphus jujube, ZjGPX gene, differential expression, stress, transgenic Arabidopsis

XIAO Rong, LUO Hui-zhen, ZHANG Xiao-juan, DENG Shu, ZHANG Chun-fen, REN Ying, MENG Yu-ping, CAO Qiu-fen, NIE Yuan-Jun. Differential Expression and Functional Analysis of Glutathione Peroxidase Gene from jujube (ZjGPX ) Under Drought and Salt Stresses[J].Scientia Agricultura Sinica, 2015, 48(14): 2806-2817.

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Differential Expression and Functional Analysis of Glutathione Peroxidase Gene from jujube (ZjGPX ) Under Drought and Salt Stresses

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