牛规模化转基因技术体系构建轨迹和展望

doi:10.3864/j.issn.0578-1752.2014.21.008

Abstract

Abstract: With the continuous development of life science and the expanding needs of industry application, transgenic technology, a basic technology of life science research, is expanding to life science industry application, which includes big domestic animal transgenic technology industrialization application. GM technology is discussed in this paper about the application and the development in transgenic cattle breeding, combining with the history of transgenic cattle in the world and the bovine transgenic breeding system construction in China. Through introduction, integration and innovation of the modern animal biotechnology, a safe, efficient and large-scale cattle transgenic technology system was established, which should improve the implement in the production of cattle and more extensive application in breeding practice. The purpose of transgenic technology for cattle breeding is improvement of the ability of disease resistance, the milk quality, the milk or meat output and the bioreactor of producing some functional proteins. At present, the scale GM technology system platform of cattle is nearly mature, which level has attained world class performance. More in-depth work will be focused on the regulation of functional gene and security control of import methods. In the future, the bovine transgenic breeding work should be led to “safe, efficient, large-scale”, which includes tissue specific promoters, optimizing codon, adjusting the genetic modification, point knock-in genes, marker free and so on. Disease-resistant and high-yielding dairy breeding is the essential direction of the development of the livestock industry. Some active functional proteins could be produced by cattle gland reactor, so establishment of a perfective efficient transgenic system for cattle is one of the prominent development direction in the future.

Key words: cattle, transgenic system, breeding, large-scale

MENG Qing-yong, LIU Chun-cheng, WANG Meng, ZHANG Kuo, DAI Yun-ping, GUO Ying, FEI Jing, LI Ning. Establishment and Prospect of Efficient Transgenic System for Cattle[J].Scientia Agricultura Sinica, 2014, 47(21): 4224-4233.

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