棉花规模化转基因技术体系构建及其应用

doi:10.3864/j.issn.0578-1752.2014.21.005

Abstract

Abstract: This assay is a summary of the application of transgenic technology in cotton all over the world, including the establishment and research progress of cotton transgenic technology for large-scale performance in China in recent years, the main problems and development trend of transgenic technology in cotton, which gives an insight into the transgenic cotton for researchers and makes a great significance to help them working efficiently. Transgenic technology has achieved great progress in cotton bollworm resistance by GMOs, and will gradually get important progresses in disease and adverse resistance in cotton, etc. Early transformation events are transformed by Coker cultivars, and nowadays rapid progresses have been made in transgenic technology for the success of different cotton species of tissue culture in Gossypium raimondii, G. arboretum, G. barbadense, G. hirsutum, etc. and three major transformation methods containing biolistic particle, pollen tube pathway and Agrobacterium-mediated transformation. Cotton transgenic technology for large-scale performance in China is established mainly by Cotton Research Institute of Chinese Academy of Agricultural Sciences and other institutes in China. It forms a trinity system for cotton large-scale transformation by focusing on the optimization of Agrobacterium-mediated method through the selection of efficient transformation vectors, transformation system establishment of major cotton varieties or lines, optimization of tissue culture conditions, in addition to the improvement of biolistic particle and pollen tube pathway methods. This transformation system involves in establishment of Agrobacterium mediated transformation system selecting CRI24 as the transgenic receptor, getting new materials such as W12 whose differentiation rate is up to 100% by petiole tissue culture screening and transformation efficiency increased by 2.88 times compared with before, development of embryonic callus transformation system by biolistic particle method, improvement of the transformation efficiency by pollen tube pathway. Using the transform system, many transgenic materials or lines are obtained and some of them are bred to insect-resistant cotton varieties, more than 200 genes for 41 scientific research institutes in China have been identified their gene functions, and provides a large number of new materials for cotton breeding. It is concluded that strong genotype-dependence is the bottleneck for cotton transformation, expanding the genotype of transgenic receptor, improving the transformation efficiency, and scaling up the system are the subjects of cotton transformation for a long time. At the same time, concerning the efficiency and the public anxiety, further studies in this field should be focused on exploring and discovering safer and more effective transformation system such as multi-gene transformation, plastid transformation, fixed-point conversion or gene stacking, developing safe or maker-free transgenic technique. At last, the strict requirement for safety evaluation needs to be further researched on the genetically modified materials. With the publication of cotton genome sequences, it will be a new direction for cotton basic and applied research to clone cotton genes, which will provide valuable information for the transformation of candidate genes and breeding of transgenic cotton varieties.

Key words: cotton, transgenic system, largescale, genotype dependence, safe transformation system

LIU Chuan-liang, TIAN Rui-ping, KONG De-pei, LI Feng-lian, SHANG Hai-hong, CHEN Xiu-jun. Establishment and Application of Efficient Transformation System for Cotton[J].Scientia Agricultura Sinica, 2014, 47(21): 4183-4197.

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