棉花规模化转基因技术体系构建及其应用

doi:10.3864/j.issn.0578-1752.2014.21.005

Abstract

Abstract: This assay is a summary of the application of transgenic technology in cotton all over the world, including the establishment and research progress of cotton transgenic technology for large-scale performance in China in recent years, the main problems and development trend of transgenic technology in cotton, which gives an insight into the transgenic cotton for researchers and makes a great significance to help them working efficiently. Transgenic technology has achieved great progress in cotton bollworm resistance by GMOs, and will gradually get important progresses in disease and adverse resistance in cotton, etc. Early transformation events are transformed by Coker cultivars, and nowadays rapid progresses have been made in transgenic technology for the success of different cotton species of tissue culture in Gossypium raimondii, G. arboretum, G. barbadense, G. hirsutum, etc. and three major transformation methods containing biolistic particle, pollen tube pathway and Agrobacterium-mediated transformation. Cotton transgenic technology for large-scale performance in China is established mainly by Cotton Research Institute of Chinese Academy of Agricultural Sciences and other institutes in China. It forms a trinity system for cotton large-scale transformation by focusing on the optimization of Agrobacterium-mediated method through the selection of efficient transformation vectors, transformation system establishment of major cotton varieties or lines, optimization of tissue culture conditions, in addition to the improvement of biolistic particle and pollen tube pathway methods. This transformation system involves in establishment of Agrobacterium mediated transformation system selecting CRI24 as the transgenic receptor, getting new materials such as W12 whose differentiation rate is up to 100% by petiole tissue culture screening and transformation efficiency increased by 2.88 times compared with before, development of embryonic callus transformation system by biolistic particle method, improvement of the transformation efficiency by pollen tube pathway. Using the transform system, many transgenic materials or lines are obtained and some of them are bred to insect-resistant cotton varieties, more than 200 genes for 41 scientific research institutes in China have been identified their gene functions, and provides a large number of new materials for cotton breeding. It is concluded that strong genotype-dependence is the bottleneck for cotton transformation, expanding the genotype of transgenic receptor, improving the transformation efficiency, and scaling up the system are the subjects of cotton transformation for a long time. At the same time, concerning the efficiency and the public anxiety, further studies in this field should be focused on exploring and discovering safer and more effective transformation system such as multi-gene transformation, plastid transformation, fixed-point conversion or gene stacking, developing safe or maker-free transgenic technique. At last, the strict requirement for safety evaluation needs to be further researched on the genetically modified materials. With the publication of cotton genome sequences, it will be a new direction for cotton basic and applied research to clone cotton genes, which will provide valuable information for the transformation of candidate genes and breeding of transgenic cotton varieties.

Key words: cotton, transgenic system, largescale, genotype dependence, safe transformation system

LIU Chuan-liang, TIAN Rui-ping, KONG De-pei, LI Feng-lian, SHANG Hai-hong, CHEN Xiu-jun. Establishment and Application of Efficient Transformation System for Cotton[J].Scientia Agricultura Sinica, 2014, 47(21): 4183-4197.

References

[1] Wilkins T A, Rajasekaran K, Anderson D M. Cotton biotechnology. Critical Reviews in Plant Sciences, 2000, 19(6): 511-550.

[2] Perlak F J, Deaton R W, Armstrong T A, Fuchs R L, Sims S R, Greenplate J T, Fischhoff D A. Insect resistant cotton plants. Biotechnology, 1990, 8: 939-943.

[3] Jenkins J N, Parrott W L, McCarty J C, Callahan F E, Berberich S A, Deaton W R. Growth and survival of Heliothis virescens (Lepidoptera, Noctuidae) on transgenic cotton containing a truncated form of the delta endotoxin gene from Bacillus thuringiensis. Journal of Economic Entomology, 1993, 86: 181-185.

[4] Nida D L, Kolacz K H, Buehler R E, Deaton W R, Schuler W R, Armstrong T. Glyphosate tolerant cotton: Characterization and protein expression. Journal of Agricultural and Food Chemistry, 1996, 44: 1960-1966.

[5] 李付广, 刘传亮. 生物技术在棉花育种中的应用. 棉花学报, 2007, 19(5): 362-368.

Li F G, Liu C L. Applications of biotechnology on the cotton breeding. Cotton Science, 2007, 19(5): 362-368. (in Chinese)

[6] Umbeck P, Johnson G, Barton K, Swain W. Genetically transformed cotton (Gossypium hirsutum L.) plants. Nature Biotechnology, 1987, 5: 263-266.

[7] Firoozabady E, DeBoer D L, Merlo D J, Halk E L, Amerson L N, Rashka K E, Murray E E. Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants. Plant Molecular Biology, 1987, 10: 105-116.

[8] Cousins Y L, Lyon B R, Llewellyn D J. Transformation of an Australian cotton cultivar: Prospects for cotton improvement through genetic engineering. Australian Journal of Plant Physiology, 1991, 18(5): 481-494.

[9] Bayley C, Trolinder N, Ray C, Morgan M, Quisenberry J E, Ow D W. Engineering 2,4-D resistance into cotton. Theoretical and Applied Genetics, 1992, 83(5): 645-649.

[10] Thomas J C, Adams D G, Keppenne V D, Wasmann C C, Brown J K, Kanost M R, Bohnert H J. Protease inhibitors of Manduca sexta expressed in transgenic cotton. Plant Cell Reports, 1995, 14(12): 758-762.

[11] Chlan C A, Lin J, Cary J W, Cleveland T E. A procedure for biolistic transformation and regeneration of transgenic cotton from meristematic tissue. Plant Molecular Biology Reporter, 1995, 13(1): 31-37.

[12] Keller G, Spatola L, Mccabe D, Martinell, B, Swain, W, John, M E. Transgenic cotton resistant to herbicide bialaphos. Transgenic Research, 1997, 6(6): 385-392.

[13] 朱卫民, 吴敬音, 佘建明, 蔡小宁, 朱祯, 李向辉. 棉花茎尖分生组织在微粒轰击法基因转化中的应用. 江苏农业学报, 1998, 14(2): 74-79.

Zhu W M, Wu J Y, She J M , Cai X N, Zhu Z, Li X H. Application of the shoot apical meristem of cotton in gene transformation by particle bombardment. Jiangsu Journal of Agricultural Sciences, 1998, 14(2): 74-79. (in Chinese)

[14] Huang G C, Dong Y M, Sun J S. Introduction of exogenous DNA into cotton via the pollen-tube pathway with GFP as a reporter. Chinese Science Bulletin, 1999, 44(8): 698-701.

[15] Zhang Z L, Ni W C, Zhang B L, Xu Y J, Guo S D, Cui H Z, Shu C E. Insect-resistant transgenic cotton plants transformed with pollen tube pathway. Acta Agriculturae Boreali-Occidentalis Sinica, 1999, 9(2): 1-4.

[16] Rajasekaran K, Hudspeth R L, Cary J W, Anderson D M, Cleveland T E. High-frequency stable transformation of cotton (Gossypium hirsutum L.) by particle bombardment of embryogenic cell suspension cultures. Plant Cell Reports, 2000, 19(6): 539-545.

[17] Chen W X, Xiao G F, Zhu Z. Obtaining high pest-resistant transgenic upland cotton cultivars carrying cry1Ac3 gene driven by chimeric OM promoter. Acta Botanica Sinica, 2002, 44(8): 963-970.

[18] Wu X, Wang J J, Zhu Z, Shangguan X X, Zhang L S, Li B, Du C F, Li Y E. Study of transgenic cotton carrying Bt-CpTI-GNA genes. Cotton Science, 2005, 17: 353-359.

[19] 刘锡娟, 刘昱辉, 王志兴, 王旭静, 张永强. 转 5-烯醇式丙酮酰莽草酸-3-磷酸合酶 (EPSPS) 基因抗草甘膦烟草和棉花的获得. 农业生物技术学报, 2007, 15(6): 958-963.

Liu X J, Liu Y H, Wang Z X, Wang X J, Zhang Y Q. Generation of glyphosate-tolerant transgenic tobacco and cotton by transformation with a 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPS) gene. Journal of Agricultural Biotechnology, 2007, 15(6): 958-963. (in Chinese)

[20] 范小平, 范博红, 李学宝, 杨维才, 徐子勤. 肌动蛋白基因在棉花纤维中的作用研究. 华北农学报, 2008, 23(5): 73-75.

Fan X P, Fan B H, Li X B, Yang W C, Xu Z Q. Transformation of GhACT1 RNAi and its effect on fiber length of cotton (Gossypium hirsutum). Acta Agriculturae Boreali-Sinica, 2008, 23(5): 73-75. (in Chinese)

[21] 李付广, 侯玉霞, 方鑫, 龚玉梅, 张雪妍. 来源于耐旱荒漠植物蛋白 CkND 对棉花黄姜病的抑制作用及其抗旱性研究. 棉花学报, 2009, 21(2): 89-93.

Li F G, Hou Y X, Fang X, Gong Y M, Zhang X Y. Studies on the inhibition of plant derived protein CkND against Verticillium dahlia and its drought resistance. Cotton Science, 2009, 21(2): 89-93. (in Chinese)

[22] Hashmi J A, Zafar Y, Arshad M, Mansoor S, Asad S. Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences. Virus Genes, 2011, 42(2): 286-296.

[23] Liu J F, Zhao C Y, Ma J, Zhang G Y, Li M G, Yan G J, Ma Z Y. Agrobacterium-mediated transformation of cotton (Gossypium hirsutum L.) with a fungal phytase gene improves phosphorus acquisition. Euphytica, 2011, 181(1): 31-40.

[24] Liu Y D, Yin Z J, Yu J W, Li J, Wei H L, Han X L, Shen F F. Improved salt tolerance and delayed leaf senescence in transgenic cotton expressing the Agrobacterium IPT gene. Biologia Plantarum, 2012, 56(2): 237-246.

[25] Liu G Z, Jin S X, Liu X Y, Tan J F, Yang X Y, Zhang X L. Overexpression of Arabidopsis cyclin D2;1 in cotton results in leaf curling and other plant architectural modifications. Plant Cell Tissue and Organ Culture, 2012, 110(2): 261-273.

[26] Xu W L, Zhang D J, Wu Y F, Qin L X, Huang G Q, Li J, Li X B. Cotton PRP5 gene encoding a proline-rich protein is involved in fiber development. Plant Molecular Biology, 2013, 82(4/5): 353-365.

[27] Rao Q, Bajwa A, Puspito K S, Khan A N, Abbas M, Bakhsh M A, Husnain T. Variation in expression of phytochrome B gene in cotton (Gossypium hirsutum L.). Journal of Agricultural. Science and Technology, 2013, 15(5): 1033-1042.

[28] Liu, Z, Zhu, Z, Zhang, T Z. Development of transgenic CryIA (c) + GNA cotton plants via pollen tube pathway method confers resistance to Helicoverpa armigera and Aphis gossypii Glover//Zhang B H. Transgenic Cotton, Methods and Protocols. New York: Humana Press, 2013: 199-210.

[29] Zhang B H, Wang M, Zhang X, Li C Q, Wang Q L. Overexpression of miR156 in cotton via Agrobacterium-mediated transformation// Zhang B H. Transgenic Cotton, Methods and Protocols. New York: Humana Press, 2013: 189-197.

[30] Kumar V, Joshi S G, Bell A A, Rathore K S. Enhanced resistance against Thielaviopsis basicola in transgenic cotton plants expressing Arabidopsis NPR1 gene. Transgenic Research, 2013, 22(2): 359-368.

[31] 欧婷, 何秋伶, 陈进红, 祝水金. 基于抗草甘膦基因的棉花茎尖农杆菌介导转化方法的研究. 棉花学报, 2013, 25(5): 410-416.

Ou T, He Q L, Chen J H, Zhu S J. Studies on the genetic transformation method by cotton shoot tip-Agrobacterium medium using the glyphosate resistant gene. Cotton Science, 2013, 25(5): 410-416. (in Chinese)

[32] Beasley C A. In vitro culture of fertilized cotton ovules. Bioscience, 1971, 21(17): 906-907.

[33] Shoemaker R C, Couche L J, Galbraith D W. Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.). Plant Cell Reports, 1986, 5(3): 178-181.

[34] 刘传亮, 武芝霞, 张朝军, 李凤莲, 王玉芬, 李付广. 农杆菌介导棉花大规模高效转化体系的研究. 西北植物学报, 2004, 5(24): 768-775.

Liu C L, Wu Z X, Zhang C J, Li F L, Wang Y F, Li F G. Study on large-scale and high efficient transformation system mediated by Agrobacterium tumefaciens of cotton. Acta Botanica Boreali- occidentalia Sinica, 2004, 24(5): 768-775. (in Chinese)

[35] Wu J H, Zhang X L, Nie Y C, Jin S X, Liang S G. Factors affecting somatic embryogenesis and plant regeneration from a range of recalcitrant genotypes of chinese cottons. In vitro Cellular and Developmental Biology-Plant, 2004, 40(4): 371-375.

[36] Sakhanokho H F, Ozias-Akins P O, Lloyd M, Chee P W. Induction of somatic embryogenesis and plant regeneration in select georgia and Pee Dee cotton Lines. Crop Science, 2004, 44(6): 2199-2205.

[37] Chaudhary B, Kumar S, Prasad K V S K, Oinam G S, Burma P K, Pental D. Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker 310 FR). Plant Cell Reports, 2003, 21(10): 955-960.

[38] Wu J H, Zhang X L, Nie Y C, Luo X L. High-efficiency transformation of Gossypium hirsutum embryogenic calli mediated by Agrobacterium tumefaciens and regeneration of insect-resistant plants. Plant Breeding, 2005, 124(2): 142-146.

[39] Jin S X, Liang S G, Zhang X L, Nie Y C, Guo X P. An efficient grafting system for transgenic plant recovery in cotton (Gossypium hirsutum L.). Plant Cell, Tissue and Organ Culture, 2006, 85(2): 181-185.

[40] Wang Y X, Wang X F, Ma Z Y, Zhang G Y, Han G Y. Somatic embryogenesis and plant regeneration from two recalcitrant genotypes of Gossypium hirsutum L.. Agricultural Sciences in China, 2006, 5(5): 323-329.

[41] Li X B, Fan X P, Wang X L, Cai L, Yang W C. The Cotton ACTIN1 gene is functionally expressed in fibers and participates in fiber elongation. The Plant Cell, 2005, 17(3): 859-875.

[42] Liu Q, Singh S P, Green A G. High-stearic and high-oleic cottonseed oils produced by hairpin RNA-mediated post-transcriptional gene silencing. Plant Physiology, 2002, 129(4): 1732-1743.

[43] Ganesan M, Jayabalan N. Evaluation of haemoglobin (erythrogen): for improved somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L. cv. SVPR 2). Plant Cell Reports, 2004, 23(4): 181-187.

[44] 刘春明, 姚敦义. 陆地棉体细胞胚发生及其细胞组织学研究. 植物学报, 1991, 33(5): 378-384.

Liu C M, Yao D Y. Studies on somatic embryogenesis and histological observation in GossypIum hirsutum L. CV. Coker 312. Journal of Integrative Plant Biology, 1991, 33(5): 378-384. (in Chinese)

[45] 夏启中, 张献龙, 聂以春, 郭小平. 撤除外源生长素诱发棉花胚性悬浮细胞程序性死亡. 植物生理与分子生物学学报, 2005, 31(1): 78-84.

Xia Q Z, Zhang X L, Nie Y C, Guo X P. With drawal of exogenous auxin induces programmed cell death of cotton embryogenic suspension cultures. Journal of Plant Physiology and Molecular Biology, 2005, 31(1): 78-84. (in Chinese)

[46] 张朝军, 李付广, 喻树讯, 范术丽, 王玉芬, 武芝侠, 李凤莲, 刘传亮. 棉花叶柄组织培养与高分化率材料选育方法: 中国, 200610089439.1[P] 2006-11-22.

Zhang C J, Li F G, Yu S X, Fan S L, Wang Y F, Wu Z X, Li F L, Liu C L. Cotton leafstalk tissue cultivation and high-differentiation cotton material selective breeding method: China, 200610089439.1[P]. 2006-11-22.

[47] Klein T M, Wolf E D, Wu R, Sanford J C. High-velocity microprojectiles for delivering nucleic acids into living cells. Nature, 1987, 327(6117): 70-73.

[48] Zhou G Y, Weng J, Zheng Y S, Huang J G, Qian S Y, Liu G L. Introduction of exogenous DNA into cotton embryos. Methods in Enzymol, 1983, 101: 433-481.

[49] Trolinder N L, Linda K. In-planta method for the production of transgenic plants: US, US5994624 A[P]. 1999-11-30.

[50] 张家明, 孙雪飘, 郑学勤, 张献龙, 赵燕, 刘金兰, 孙济中. 陆地棉愈伤诱导及胚胎发生能力的遗传分析. 中国农业科学, 1997, 30(3): 36-43.

Zhang J M, Sun X P, Zheng X Q, Zhang X L, Zhao Y, Liu J L, Sun J Z. Genetic analysis of callus induction and somatic embryogenesis in upland cotton. Scientia Agricultura Sinica, 1997, 30(3): 36-43. (in Chinese)

[51] 张朝军, 王晔, 王玉芬, 李凤莲, 李付广. 棉花叶柄分化率主基因+多基因混合遗传分析. 棉花学报, 2012, 24(1): 3-9.

Zhang C J, Wang Y, Wang Y F, Li F L, Li F G. Mixed major gene and polygene inheritance analysis of embryogenesis callus induction ratio in upland cotton mature leaf petioles as explants. Cotton Science, 2012, 24(1): 3-9. (in Chinese)

[52] 商海红. 陆地棉体细胞胚胎发生的细胞学研究[D]. 北京: 中国农业科学院, 2009.

Shang H H. Cytological studies on the somatic embryogenesis of Gossypium hirsutum L. [D]. Beijing: Chinese Academy of Agricultural Sciences, 2009. (in Chinese)

[53] Gawel N J, Robacker C D. Somatic embryogenesis in two Gossypium hirsutum L. genotypes on semi-solid versus liquid proliferation media. Plant Cell, Tissue and Organ Culture, 1990, 23(3): 201-204.

[54] Xu Z Z, Zhang C J, Zhang X Y, Liu C L, Wu Z X, Yang Z R, Zhou K H, Yang X J, Li F G. Transcriptome profiling reveals auxin and cytokinin regulating somatic embryogenesis in different sister lines of cotton cultivar CCRI24. Journal of Integrative Plant Biology, 2013, 55(7): 631-642.

[55] 李静, 韩秀兰, 沈法富, 刘莲. 提高棉花花粉管通道技术转化率的研究. 棉花学报, 1999, 17(2): 67-71.

Li J, Han X L, Shen F F, Liu L. Study on promoting the pollen-tube pathway transformation in cotton. Cotton Science, 2005, 17(2): 67-71. (in Chinese)

[56] 马盾, 黄乐平, 黄全生, 孟庆玉, 危晓薇, 陈勋基, 美丽古丽. 提高棉花花粉管通道法转化率的研究. 西北农业学报, 2005, 14(1): 10-12.

Ma D, Huang L P, Huang Q S, Meng Q Y, Wei X W, Chen X J, Meili G L. Study on improvement of pollen tube pathway transformation efficiency through field concrete operation. Acta Agriculturae Boreali-Occidentalis Sinica, 2005, 14(1): 10-12. (in Chinese)

[57] 王志才, 艾尔肯木合热皮亚, 廖茂森, 张富春. 影响棉花体细胞胚胎发生和植株再生的关键因素分析. 新疆农业科学, 2011, 48(1): 39-44.

Wang Z C, Aierken M, Liao M S, Zhang F C. Analysis on key factors affecting somatic embryogenesis and plant regeneration in cotton. Xinjiang Agricultural Science, 2011, 48(1): 39-44. (in Chinese)

[58] Jin S X, Zhang X L, Liang S G, Nie Y C, Guo X P, Huang C. Factors affecting transformation efficiency of embryogenic callus of upland cotton (Gossypium hirsutum) with Agrobacterium tumefaciens. Plant Cell, Tissue and Organ Culture, 2005, 81(2): 229-237.

[59] Salm T V D, Bosch D, Honee G, Feng L X, Munsterman E, Bakker P, Stiekema W J, Visser B. Insect resistance of transgenic plants that express modified Bacillus thuringiensis cryIA(b) and cryIC genes: A resistance management strategy. Plant Molecular Biology, 1994, 26(1): 51-59.

[60] Mullen J, Adam G, Blowers A, Earle E. Biolistic transfer of large DNA fragments to tobacco cells using YACs retrofitted for plant transformation. Molecular Breeding, 1998, 4(5): 449-457.

[61] Hamilton C M. A binary-BAC system for plant transformation with high-molecular-weight DNA. Gene, 1997, 200(1): 107-116.

[62] Paszkowski J, Baur M, Bogucki A, Potrykus I. Gene targeting in plants. The EMBO Journal, 1988, 7(13): 4021-4026.

[63] Kempin S A, Liljegren S J, Block L M, Rounsley S D, Yanofsky M F, Lam E. Targeted disruption in Arabidopsis. Nature, 1997, 389(6653): 802-803.

[64] Terada R, Urawa H, Inagaki Y, Tsugane K, Iida S. Efficient gene targeting by homologous recombination in rice. Nature Biotechnology, 2002, 20(10): 1030-1034.

[65] Zhu T, Peterson D J, Tagliani L, Clair G S, Baszczynski C L, Bowen B. Targeted manipulation of maize genes in vivo using chimeric RNA/DNA oligonucleotides. Proceedings of the National Academy of Sciences of the USA, 1998, 96(15): 8768-8773.

[66] Boynton J E, Gillham N W, Harris E H, Hosler J P, Johnson A M, Jones A R, Randolph-Anderson B L, Robertson D, Klein T M, Shark K B. Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science, 1988, 240(4858): 1534-1538.

[67] Maliga P. Progress towards commercialization of plastid transformation technology. Trends in Biotechnology, 2003, 21(1): 20-28.

[68] Kumar S, Dhingra A, Daniell H. Stable transformation of the cotton plastid genome and maternal inheritance of transgenes. Plant Molecular Biology, 2004, 56: 203-216.

[69] Darbani B, Eimanifar A, Stewart C N, Camargo W N. Methods to produce marker-free transgenic plants. Biotechnology Journal, 2007, 2(1): 83-90.

[70] Ding Z S, Zhao M, Jing Y X, Li L B, Kuang T Y. Efficient Agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection. Plant Molecular Biology Reporter, 2006, 24: 295-303.

[71] Endo S, Kasahara T, Sugita K, Matsunaga E, Ebinuma H. The isopentenyl transferase gene is effective as a selectable marker gene for plant transformation in tobacco (Nicotiana tabacum cv. Petite Havana SRI). Plant Cell Reports, 2001, 20: 60-66.

[72] Zhu Z G, Wu R. Regeneration of transgenic rice plants using high salt for selection without the need for antibiotics or herbicides. Plant Science, 2008, 174(5): 519-523.

[73] Daniell H, Muthukumar B, Lee S B. Marker free transgenic plants: Engineering the chloroplast genome without the use of antibiotic selection. Current Genetics, 2001, 39(2): 109-116.

[74] 杨晓杰, 刘传亮, 张雪妍, 李付广. 转基因植物中T-DNA整合的分子特征及表达. 基因组学与应用生物学, 2010, 29(1): 125-130.

Yang X J, Liu C L, Zhang X Y, Li F G. Molecule characterization and expression of T-DNA integration in transformed plants. Genomics and Applied Biology, 2010, 29(1): 125-130. (in Chinese)

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Establishment and Application of Efficient Transformation System for Cotton

RichHTML

PDF

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	LI YuanJing, YUAN RuiXiang, LI YongTai, SUN TianGe, LIU Feng, LI YanJun, ZHANG XinYu. Identification and Functional Characterization of β-Glucosidase Genes in Verticillium dahliae for Pathogenicity on Cotton [J]. Scientia Agricultura Sinica, 2026, 59(7): 1380-1399.
[2]	YAN TingLin, DU YaDan, HU XiaoTao, WANG He, LI XiaoYan, WANG YuMing, NIU WenQuan, GU XiaoBo. The Impacts of Nitrogen Fertilizer Organic Alternatives Under Aerated Drip Irrigation on Cotton Yield and Water Use Efficiency Under Deficit Irrigation Conditions [J]. Scientia Agricultura Sinica, 2026, 59(3): 602-618.
[3]	GUO ChenLi, LIU Yang, CHEN Yan, HU Wei, WANG YouHua, ZHOU ZhiGuo, ZHAO WenQing. Effects of Phosphorus Fertilizer Postpone Under Nitrogen Reduction Condition on Yield, Phosphorus Fertilizer Utilization Efficiency of Drip-Irrigated Cotton [J]. Scientia Agricultura Sinica, 2025, 58(9): 1749-1766.
[4]	WANG WeiMeng, WEI YunXiao, TANG YunNi, LIU MiaoMiao, CHEN QuanJia, DENG XiaoJuan, ZHANG Rui. Establishment and Rooting Optimization of Agrobacterium rhizogenes Transformation System in Cotton [J]. Scientia Agricultura Sinica, 2025, 58(8): 1479-1493.
[5]	ZHAO YuXuan, MIAO JiYuan, HU Wei, ZHOU ZhiGuo. Effects of Low Temperature at Seedling Stage on Cotton Floral Bud Differentiation and Cotton Plant Yield [J]. Scientia Agricultura Sinica, 2025, 58(7): 1311-1320.
[6]	TIAN LiWen, LOU ShanWei, ZHANG PengZhong, DU MingWei, LUO HongHai, LI Jie, PAHATI MaiMaiTi, MA TengFei, ZHANG LiZhen. Analysis of Problems and Pathways for Increasing Cotton Yield per Unit Area in Xinjiang Under Green and Efficient Production Mode [J]. Scientia Agricultura Sinica, 2025, 58(6): 1102-1115.
[7]	WANG LiYuan, WANG Hui, WANG MuMu, WANG DongJian, LI RuYu, ZHENG YongSheng, ZHANG Han. Construction and Application of DNA Fingerprint Database for Known Varieties in Upland Cotton DUS Testing [J]. Scientia Agricultura Sinica, 2025, 58(22): 4570-4588.
[8]	TANG ChaoYuan, LIU TaoFen, WU YanQin, ZHANG QiPeng, LI ZiLiang, CHEN YunRui, LEI ZhangYing, ZHANG YaLi, ZHANG WangFeng, DU MingWei, YANG MingFeng, TIAN JingShan. Relationship Between Boll Morphological Characteristics and Fiber and Kernel Quality of Gossypium hirsutum L. and Gossypium barbadense L. [J]. Scientia Agricultura Sinica, 2025, 58(15): 2980-2992.
[9]	WEN Jin, NING YanFang, QIN Xin, LIU Yuan, ZHANG XiaoLing, ZHU YongHong, TIAN ShiMin, MA YanBin. Resistance Evaluation and Genetic Stability Analysis of Insect- Resistant and Glyphosate-Tolerant Transgenic Cotton Lines [J]. Scientia Agricultura Sinica, 2025, 58(12): 2291-2302.
[10]	DONG Ming, QI Hong, ZHANG Qian, WANG Yan, WANG ShuLin, FENG GuoYi, LIANG QingLong, GUO BaoSheng. The Impact of Sowing Methods on the Seed Germination Environment and Cotton Emergence and Growth [J]. Scientia Agricultura Sinica, 2025, 58(12): 2346-2357.
[11]	ZHANG YanJun, DAI JianLong, DONG HeZhong. On Multi-Objective Collaborative Cultivation in Cotton Production [J]. Scientia Agricultura Sinica, 2025, 58(10): 1908-1916.
[12]	WU YuZhen, HUANG LongYu, ZHOU DaYun, HUANG YiWen, FU ShouYang, PENG Jun, KUANG Meng. Construction of SSR Fingerprint Library and Comprehensive Evaluation for Approved Cotton Varieties in China [J]. Scientia Agricultura Sinica, 2024, 57(8): 1430-1443.
[13]	LEI JianFeng, YOU YangZi, ZHANG JinEn, DAI PeiHong, YU Li, DU ZhengYang, LI Yue, LIU XiaoDong. Screening of High-Efficient sgRNA for Targeted Knockout of GhAGL16 Gene in Cotton [J]. Scientia Agricultura Sinica, 2024, 57(6): 1023-1033.
[14]	LI KaiLi, WEI YunXiao, CHONG ZhiLi, MENG ZhiGang, WANG Yuan, LIANG ChengZhen, CHEN QuanJia, ZHANG Rui. Red and Blue Light Promotes Cotton Callus Induction and Proliferation [J]. Scientia Agricultura Sinica, 2024, 57(4): 638-649.
[15]	ZHANG YongLi, ZHANG Ning, XU Jiao, XU DouDou, CHENG Fang, ZHANG ChengLong, WU BiBo, GONG YangCang, HE YunXin, WEI ShangZhi, TU XiaoJu, LIU AiYu, ZHOU ZhongHua. Effects of Different Strip Intercropping Patterns on the Growth and Productivity in Cotton [J]. Scientia Agricultura Sinica, 2024, 57(22): 4444-4458.