Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (11): 2088-2098.doi: 10.3864/j.issn.0578-1752.2014.11.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Construction of a Genetic Linkage Map in Mungbean

 WU  Chuan-Shu-1, 2 , WANG  Li-Xia-2, WANG  Su-Hua-2, CHEN  Hong-Lin-2, WU  Jian-Xin-2, CHENG  Xu-Zhen-2, YANG  Xiao-Ming-1, 3   

  1. 1、College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070;
    2、Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081;
    3、Institute of Crops, Gansu Academy of Agricultural Sciences, Lanzhou 730070
  • Received:2013-12-15 Online:2014-06-06 Published:2014-02-21

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Abstract: 【Objective】On the basis of previous studies, a genetic map of mungbean was constructed by using genome SSR, EST-SSR, STS primers of mungbean and common bean to build a platform for positioning important traits related genes, cloning and molecular marker-assisted breeding of new varieties of mungbean.【Method】A total of 6 686 SSR, EST-SSR, STS primers of mungbean and common bean, including 6 100 genome SSR, 149 EST-SSR, 13 STS primer pairs of mungbean, and 424 genome SSR primers of common bean, were used for PCR amplification to screen polymorphic markers between Australia-imported Berken (highly susceptible cultivar)×ACC41 (highly resistant wild species) and a RIL derived from these two genotypes were tested with the polymorphic markers. Combined with the molecular marker data of previous studies, Mapmarker/Exp 3.0 software was used for map construction and set LOD≥3.0, the maximum figure at 50.00 cM. Finally, Joinmap 4.0 software was used for map integration.【Result】In this study, from the two parents, 6 686 SSR primers were screened, a total of 3 691 pairs of primers were amplified stable products, 588 pairs of polymorphic primers were obtained. Among them, mungbean SSR primers 6 100 pairs, effective amplification 3 459 pairs, the effective rate of 56.7%, obtained 559 pairs of polymorphic primers; Mungbean EST-SSR primers 149 pairs, effective amplification 126 pairs, the effective rate of 84.6%, obtained 21 pairs of polymorphic primers; Common bean SSR primers 424 pairs, effective amplification 97 pairs, the effective rate of 22.9%, obtained 6 pairs of polymorphic primers; Mungbean STS primers 13 pairs, effective amplification 9 pairs, the effective rate of 69.2%, obtained 2 pairs of polymorphic primers; These results indicated that different sources and types of SSR primers to amplify the effective rate of the parent were significantly different. Mungbean EST-SSR primers (84.6%) were the highest, mungbean STS primers (69.2%) and mungbean SSR primers (55.7%) followed, common bean SSR primers (22.9%) were the lowest. An integrated genetic linkage map of mungbean containing 585 markers was constructed (including 499 SSR markers, 74 RAPD markers, 9 STS markers and 3 RAPD markers). The total length of the map was 732.9 cM and covered 11 linkage groups. The average distance between markers was 1.25 cM. The average distance of each linkage group spanned 66.63 cM. The average number of markers was 53.18 for each of 11 chromosomes. The length of each linkage group ranged from 35 to 92 markers was from 45.2 cM to 112.8 cM. LG1 linkage group contained the largest number of 92 markers, the length was 112.8 cM. LG11 linkage group contained the minimum number of 35 markers, the length was 48.7 cM. Of the 585 markers loci mapping conducted χ2 tests under P＜0.05 and P＜0.01 conditions, respectively, 79 and 151 markers showed a segregation distortion, the total number of sites marked 39.3%.【Conclusion】A linkage map of mungbean with a maximum and the highest density genetic markers was constructed compared with that published at home and abroad at present.

Key words: mungbean , genetic linkage map , marker

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