小麦TaSnRK2.10的多态性及与农艺性状的关联

doi:10.3864/j.issn.0578-1752.2014.10.001

Abstract

Abstract: 【Objective】 The sucrose non-fermenting protein kinase (SnRK) is a kind of Ser/Thr protein kinase found widely in plants and participates a variety of transductions in signaling pathway in plants. TaSnRK2.10 is an important SnRK2 member involved in response to various abiotic stresses in wheat (Triticum aestivum L.). The objective of this study is to detect the single nucleotide polymorphism (SNP) of TaSnRK2.10, develop and map its functional markers, analyze the relationship between its haplotypes and phenotypic traits, and provide a basis for the genetic improvement and germplasm enhancement by molecular marker assisted selection in wheat. 【Method】 Thirty hexaploid wheat accessions with high polymorphism and their diploid and tetraploid wild relative species were selected to detect the nucleotide polymorphism in TaSnRK2.10-A gene by sequencing. A set of Chinese Spring nulli-tetrasomic lines and the recombinant inbred lines (RIL) derived from a cross of “Yanzhan 1 × Neixiang 188” were used to map TaSnRK2.10-A on chromosome. Based on the polymorphism in the sequence of TaSnRK2.10-A molecular markers were developed. The relevance between TaSnRK2.10-A haplotypes and phenotypic traits was analyzed using a natural population consisted of 262 historical wheat accessions. 【Result】 The sequences of TaSnRK2.10 on genomes A and D were cloned, named as TaSnRK2.10-A and TaSnRK2.10-D. There was no SNP detected in the sequence of TaSnRK2.10-D, but the full length of TaSnRK2.10-A was 4 688 bp with 15 SNPs and 2 InDels. Among them, 8 SNPs were identified in the promoter region, 2 SNPs in 5′-UTR region, and 5 SNPs in the coding region with 2 SNPs in exon. One of SNPs in exon was non-synonymous mutation. Four markers were developed. They were PM1 and PM2 for the promoter region, GM1 and GM2 for the coding region. TaSnRK2.10-A was mapped in the intervals between markers Xwpt7001 and WMC48 on chromosome 4A, with 5.1 cM and 25.7 cM from the flanking markers. In the natural populations consisted of 262 accessions, four haplotypes of TaSnRK2.10-A were detected by four markers that were associated with 1000-grain weight, spike per plant and spikelet per spike. The HapⅡand HapⅢ of TaSnRK2.10-A are considered as potential superior haplotypes for the improvement of 1000-grain weight. The base C at the site of 4 184 bp is a superior allele for high 1000-grain weight. 【Conclusion】 The present research mapped TaSnRK2.10-A on the chromosome 4A. The HapⅡand HapⅢ of TaSnRK2.10-A are considered as potential superior haplotypes for the improvement of 1000-grain weight, while HapⅣ is a potential superior haplotype for spike per plant. The cytosine (C) at the position of 4 184 bp is the superior allele.

Key words: wheat , TaSnRK2.10 , SNP , functional marker , haplotype , association analysis

WANG Qian-1, 2 , MAO Xin-Guo-2, CHANG Xiao-Ping-2, JIA Ji-Zeng-2, LIU Hui-Min-1, JING Rui-Lian-2. Polymorphism of TaSnRK2.10 and Its Association with Yield-Related Traits in Wheat[J].Scientia Agricultura Sinica, 2014, 47(10): 1865-1877.

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Polymorphism of TaSnRK2.10 and Its Association with Yield-Related Traits in Wheat

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