湖羊BMP7基因遗传多态、表达及与羔皮毛囊性状的关联

doi:10.3864/j.issn.0578-1752.2014.09.016

Abstract

Abstract: 【Objective】 The article aims to study the influence of BMP7 on the formation of Hu sheep’s different patterns and the possible regulatory mechanisms on the development of hair follicles. 【Method】 BMP7 gene in a sample of 205 single nucleotide polymorphisms in exons 1,2 and 3 were detected by PCR-SSCP. Hu sheep’s hair follicles were observed by histology and microscopic examination, statistical analysis on the diameter of primary and secondary hair follicles, the number of primary and secondary hair follicles were statistically analyzed. The relative expression of BMP7 among large, medium and small waves was detected by fluorescence quantitative PCR, and SPSS 17.0 software was used to conduct the significant difference analysis, to investigate the BMP7 gene’s regulatory mechanisms of the formation of Hu sheep’s different patterns and hair follicles traits. 【Result】 The results showed that by using PCR-SSCP method for detecting the single nucleotide polymorphisms of BMP7 gene’s 3 exons and no polymorphism was found. In histological analysis, the diameter of large waves primary hair follicles was more than medium and small waves, the diameter of small waves primary and secondary hair follicles was between large and medium waves. The number of large waves secondary hair follicles was more than medium and small waves, while the small waves were between large and medium waves. Through significant difference analysis, the difference in diameter of primary follicles in large waves was highly significant with medium and small waves (P＜0.01), the difference in diameter of primary follicle between large and small waves was not significant (P＞0.05). The difference in diameter of secondary follicles in medium waves was highly significant with large and small waves (P＜0.01), but the difference in diameter of secondary follicle between large and small waves was not significant (P＞0.05). The difference in number of primary follicles among large, medium, and small waves was not significant (P＞0.05), but in the same vision, the number of primary follicles among large waves was higher than medium and small waves. The difference in number of secondary follicles in medium waves was highly significant with large and small waves (P＜0.01), but the difference in number of secondary follicles between large and small waves was not significant (P＞0.05). qRT-PCR results showed that in the same period, the expression of BMP7 in small waves was higher than large waves, and showed a significant difference between large and small waves (P＜0.05). The relative expression of BMP7 was significantly correlated with the primary follicle diameter in small waves, and showed a highly significant positive correlation (P＜0.01)with the number of small waves’ secondary follicles, and negatively correlated with middle waves’ primary follicle diameter, the number of secondary follicle (P＜0.05). The expression was consistent with biopsy results. 【Conclusion】 Single nucleotide polymorphism was not found in BMP7 gene, however, the expression of BMP7 was correlated with hair follicles indexes and consisted with the histological observation. It is speculated that BMP7 gene may be involved in the development of hair follicle and regulation of hair growth.

Key words: Hu sheep , BMP7 , polymorphism , hair follicle

YIN Jin-Feng-1, NI Rong-1, WANG Qing-Zeng-1, SUN Wei-1, DING Jia-Tong-1, ZHANG You-Fa-2, CHEN Ling-2, WU Wen-Zhong-2, ZHOU Hong-3. The Genetic Polymorphism, Expression of BMP7 Gene and Its Relationship with Lamb Skin Follicle Traits in Hu Sheep[J].Scientia Agricultura Sinica, 2014, 47(9): 1811-1818.

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References

[1]席斌, 高雅琴, 杜天庆. 影响中国小湖羊皮品质的因素及建议. 黑龙江畜牧兽医, 2007, 11: 48-50.

Xi B, Gao Y Q, Du T Q. The factors and suggestions of affecting Hu-sheep skin quality. Heilongjiang Animal Husbandry and Veterinary Medicine, 2007, 11: 48-50. (in Chinese)

[2]席斌, 高雅琴, 李维红. 我国湖羊的发展现状及前景. 畜牧兽医杂志, 2007, 26(5): 37-41.

Xi B, Gao Y Q, Li W H. The development status and prospects of the Hu-sheep. Journal of Animal Science and Veterinary Medicine, 2007, 26(5): 37-41. ( in Chinese)

[3]Nilsson O S, Urist M R, Dawson E G, Schmalzried T P, Finerman G A M. Bone repair induced by bone morphogenetic protein in Ulnar defects in dogs. The Journal of Bone and Joint Surgery, 1986, 68: 635-642.

[4]乌日汗, 敖强. 骨形态发生蛋白研究进展. 内蒙古民族大学学报:自然科学版, 2011, 26(2): 203-206.

Wu R H, Ao Q. Research progress of bone morphogenetic protein. Journal of Inner Mongolia University for Nationalities: Natural Sciences, 2011, 26(2): 203-206. ( in Chinese)

[5]何晓曼. BMP家族成员在毛囊生长发育方面的研究. 新疆畜牧业, 2011(6): 36-37.

He X M. The research of BMP family members in hair follicle growth and development aspects. Xinjiang Animal Husbandry, 2011(6): 36-37. (in Chinese)

[6]赵甜娜, 韩金祥, 王世立. 骨形态发生蛋白-7的研究进展. 药物生物技术, 2005,12(5):328-331.

Zhao T N, Han J X, Wang S L. The research progress of bone morphogenetic protein-7. Pharmaceutical Biotechnology, 2005, 12(5): 328-331. ( in Chinese)

[7]Noramly S, Morgan B A. BMPs mediate lateral inhibition at successive stages in feather tract development. Development，1998(125): 3775-3787.

[8]吴贤杰, 郑敏, 吕中法. 骨形成蛋白在毛囊中表达意义的研究进展. 浙江大学学报: 医学版, 2004, 33(4): 370-374.

Wu X J, Zheng M, Lv Z F. The research progress of the significance of bone morphogenetic protein expression in the hair follicle. Journal of Zhejiang University: Medical Sciences, 2004, 33(4): 370-374. (in Chinese)

[9]吴江鸿, 张文广, 李金泉, 闫祖威. 内蒙古绒山羊毛囊发育不同时期皮肤中BMP4基因的表达. 黑龙江畜牧兽医, 2009, 23: 35-36.

Wu J H, Zhang W G, Li J Q, Yan Z W. The expression of BMP4 gene in the skin of different period hair follicle in Inner Mongolia cashmere goat. Heilongjiang Animal Science and Veterinary Medicine, 2009, 23: 35-36. (in Chinese)

[10]李志农, 张仲伦, 杜建中, 韩安, 常玉兰, 李天禧, 张泉福, 张荣生. 湖羊羔皮花纹形成的形态学观察. 中国农业科学, 1982, 15(3): 73-81.

Li Z N, Zhang Z L, Du J Z, Han A, Chang Y L, Li T X, Zhang Q F, Zhang R S. The morphological observation of Hu sheep lamb pattern formation. Scientia Agricultura Sinica, 1982, 15(3): 73-81. (in Chinese)

[11]许竟武, 杜白, 程瑞禾, 茅亨, 丁云清, 吴健民. 湖羊胎儿期皮肤和毛囊发生发育的研究. 中国畜牧杂志, 1980(1):2-6.

Xu J W, Du B, Cheng R H, Mao H, Ding Y Q, Wu J M. The research of Hu sheep fetal skin and hair follicle development. Chinese Journal of Animal Husbandry, 1980(1):2-6. (in Chinese)

[12]程瑞禾, 路步高, 谢成侠, 许竞武, 杜白, 吕宝铨, 张士元. 湖羊胎儿期皮肤和毛囊发生的研究. 南京农业大学学报, 1981(4): 96-108.

Cheng R H, Lu B G, Xie C X, Xu J W, Du B, Lv B Q, Zhang S Y. The research of Hu sheep fetal skin and hair follicles. Journal of Nanjing Agricultural University, 1981(4):96-108. (in Chinese)

[13]孙伟, 倪蓉, 殷金凤, 丁家桐, 张有法, 陈玲, 吴文忠, 周洪. 湖羊不同花纹皮肤组织差异表达基因的筛选. 中国农业科学, 2013, 46(2): 376-384.

Sun W, Ni R, Yin J F, Ding J T, Zhang Y F, Chen L, Wu W Z, Zhou H. Screening differentially expressed genes of skin tissue of different flowers patterns of Hu sheep. Scientia Agricultura Sinica, 2013, 46(2): 376-384. (in chinese)

[14]唐晓慧. 藏绵羊毛囊不同发育时期miRNAs表达变化研究[D]. 武汉: 华中农业大学, 2012.

Tang X H. The study of expression of miRNAs in wool follicle during different development phases[D]. Wuhan: Huazhong Agricultural University, 2012. (in Chinese)

[15]Oshinmori N, Fuchs E. Paracrine TGF-β signaling counterbalances BMP-mediated repression in hair follicle stem cell activation. Cell Stem Cell, 2012, 10(1): 63-75.

[16]Botchkarev V A, Sharov A A. BMP signaling in the control of skin development and hair follicle growth. Differentitation, 2004, 72(9): 512-526.

[17]程博涵, 冷丽, 王守志, 陈曦, 李辉. BMP7基因多态性与肉鸡生长和体组成性状的关联研究. 中国家禽, 2013, 35(3): 6-10.

Cheng B H, Leng L, Wang S Z, Chen X, Li H. Association between polymorphism of BMP7 gene and growth and body composition traits in broiler chickens. China Poultry, 2013, 35(3): 6-10. (in Chinese)

[18]Kazuhiro S, Javed M. Gene ontology annotation as text categorization: an empirical study. Information Processing and Management, 2008, 44: 1754-1770.

[19]葛燕, 张家骅. 骨形态发生蛋白在动物繁殖上的研究进展. 动物医学进展, 2006, 27(6): 33-37.

Ge Y, Zhang J H. Progress on bone morphogenetic proteins on animal reproduction. Progress in Veterinary Medicine, 2006, 27(6): 33-37. ( in Chinese)

[20]韦涛. 湖羊BMP4基因克隆、表达与多态性分析[D]. 南京：南京农业大学, 2012.

Wei T. Cloning expression and polymorphism analysis of BMP4 gene in Hu-sheep[D]. Nanjing:Nanjing Agricultural University, 2012. (in Chinese)

[21]储明星, 周文然, 孙少华, 方丽, 叶素成. 小尾寒羊 BMP4 基因多态性及其与高繁殖力关系. 农业生物技术学报, 2008, 16(2): 237-241.

Chu M X, Zhou W R, Sun S H, Fang L, Ye S C. Polymorphism of BMP4 gene and its relationship with prolificacy of Small Tail Han sheep. Journal of Agricultural Biotechnology, 2008, 16(2): 237-241. (in Chinese)

[22]王鑫磊. 黄牛GLI2、BMP7基因多态性及其与生长性状的关联分析[D]. 杨凌：西北农林科技大学, 2009.

Wang X L. Studies on polymorphisms of GLI2 and BMP7 genes and their associations with growth traits in cattle[D]. Yangling: Northwest Science-Technology University of Agriculture and Forestry, 2009. (in Chinese)

[23]李新建, 吕刚, 李改英, 任广志. BMP7基因单核苷酸多态性与猪繁殖性状的关联分析. 西北农业学报, 2013, 22(3): 6-10.

Li J X, Lv G, Li G Y, Ren G Z. Association between polymorphism of BMP7 and reproductive traits of pigs. Acta Agriculturae Boreali- occidentalis Sinica, 2013, 22(3): 6-10. (in Chinese)

[24]牟玉婷, 吴伟, 孙永峰, 宋佳巍, 孙远, 吴文立, 李瑶. BMP2基因在鹅胸、腹、背部皮肤中的定位定量表达研究. 吉林农业大学学报, 2013, 35(2): 241-244, 249.

Mu Y T, Wu W, Sun Y F, Song J W, Sun Y, Wu W L, Li Y. Research on the positioning and quantitative expression of bmp2 gene in chest, abdomen and back skin of goose. Journal of Jilin Agricultural University, 2013, 35(2): 241-244, 249. (in Chinese)

[25]苏蕊, 李金泉, 张文广, 尹俊, 赵珺, 常子丽. 骨形态发生蛋白 2（BMP2）基因在内蒙古绒山羊不同时期皮肤毛囊中的表达. 中国农业科学, 2008, 41(2): 559-563.

Su R, Li J Q, Zhang W G, Yin J, Zhao J, Chang Z L. Expression of BMP2 in the skin and hair follicle from different stage in Inner Mongolia Cashmere goat. Scientia Agricultura Sinica, 2008, 41(2): 559-563. (in Chinese)

[26]毛青青, 代蓉, 沈思军, 郭洪, 万鹏程, 周平, 石国庆. BMP4对小鼠毛囊数量的影响. 西北农业学报, 2012, 21(5): 13-17.

Mao Q Q, Dai R, Chen S J, Guo H, Wan P C, Zhou P, Shi G Q. Influence of BMP4 on hair follicles quantity of mice. Acta Agriculturae Boreali-occidentalis Sinica, 2012, 21(5): 13-17. (in Chinese)

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