Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (22): 4665-4678.doi: 10.3864/j.issn.0578-1752.2013.22.004

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Effect of Post-Anthesis Alternate Wetting and Drying Irrigation on Protein Expressions in Superior and Inferior Spikelets of Rice

 CHEN  Ting-Ting, CHU  Guang, HUA  Xiao-Long, PAN  Yan, WANG  Zhi-Qin, YANG  Jian-Chang   

  1. College of Agriculture, Yangzhou University/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou 225009, Jiangsu
  • Received:2013-03-18 Online:2013-11-15 Published:2013-05-29

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Abstract: 【Objective】This study aimed to understand the mechanism underlying the effect of alternate wetting and drying irrigation on grain filling of rice. 【Method】Two rice cultivars, Liangyoupeijiu (two-line indica hybrids) and Yangjing 4038 (japonica), were grown in cement tanks. Three irrigation regimes, conventional irrigation (CI), alternate wetting and moderate drying irrigation (WMD) and alternate wetting and severe drying irrigation (WSD), were imposed from full heading to maturity. The expressions of the proteins in superior and inferior spikelets were investigated using the method of two-dimensional gel electrophoresis.【Result】No significant differences in grain-filling rate and grain weight were observed for the earlier-flowering superior spikelets among the three irrigation regimes. For the later-flowering inferior spikelets, however, their grain-filling rate and grain weight were significantly increased under the WMD, but significantly reduced under the WSD when compared with those under the CI. Both WMD and WSD showed no significant impact on the protein expressions in superior spikelets. However, the WMD enhanced expressions of many proteins, such as orthophosphate dikinase, glyceraldehyde-3-phosphatedehydrogenase, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase, S-adenosylmethionine synthetase, glyoxalase I and manganese superoxide dismutase in inferior spikelets, whereas the WSD inhibited expressions of these proteins. The WSD also up-regulated expressions of some proteins, such as the proteins related to the inhibition of signal transduction and energy metabolism. The two cultivars exhibited the same tendencies. 【Conclusion】 Expression differences of the proteins related to grain filling in inferior spikelets account for their increases or decreases in grain-filling rate and grain weight under the WMD or WSD.

Key words: rice , alternate wetting and drying irrigation , inferior spikelets , grain filling , protein expression

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