Abstract: 【Objective】 This study constructed the total flavonoids characteristic constituents HPLC fingerprints of Fengjie navel orange (Citrus sinensis Osbeck ) came from its original birthplace. And authenticity of the 5 popular commercial sweet orange were tested compared to its standard HPLC fingerprints in order to provide a scientific evidence for quality evaluation and genuine identification of the citrus fruit. 【Method】 By High Performance Liquid Chromatography-Photo-Diode Array Detector (HPLC-PDA), the study carried out advancing and HPLC detecting of the total flavonoids characteristic constituents in Fengjie navel orange samples from 14 main different producing places, then the signal acquiring and the characteristic constituents peaks distinguishing was performed by the PDA and Shimadzu LC solution Ver 1.0, respectively. The hesperidin and naringin contents in peel, flesh and juice of the samples were detected by external standard method. At last the total flavonoids characteristic constituents HPLC fingerprints from different parts of the samples were constructed and their similarity were calculated and analysed by the professional similarity evaluation software including validation study. 【Result】 Results showed that hesperidin content range in the peel, flesh and juice of the samples was 1.67%-3.76%, 0.69%-1.67% and 0.022-0.318 mg•(10mL)-1, the mean value was 2.87%, 1.25% and 0.1215 mg•(10mL)-1, respectively. The naringin content range of fruits peel was 0.01%-0.15%, and its average value was 0.08%. However, they were not detected in their flesh and juice. The chromatographic peaks shape of total flavonoids in peel and flesh of different samples was generally coherent. And their common peaks were well matched and the number was 35 and 35 irespectively. Peaks distributing difference of the constructed characteristic chromatographic fingerprint of peel and flesh was obviously different. The relative content data of their common peaks was stable with reference of hesperidin peak. The similarity of samples calculated by the vector angel cosine all was above 0.990 with their characteristic HPLC fingerprint. So both of them could be used to its peculiar evaluate and genuine identification. But there was an obvious difference among the chromatographic fingerprints of the Fengjie navel orange juice and low peaks-matching rate, so the samples’ juice total flavonoids characteristic fingerprint was unsuitable for utility. 【Conclusion】 This method is accurate, reliable and well reproducible, and it is suitable for peculiar identification of Fengjie navel orange fruits well known in China and quality evaluation of the other domestic citrus cultivars.

Key words: Fengjie navel orange(Citrus sinensis Osbeck) , flavonoids , HPLC-PDA , characteristic constituents , HPLC fingerprint