Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (16): 3413-3423.doi: 10.3864/j.issn.0578-1752.2013.16.012

• HORTICULTURE • Previous Articles     Next Articles

Screening of ‘Jincheng’ Orange CTV Response Genes Using Subtractive Suppression Hybridization

 CHENG  Chun-Zhen-12, ZENG  Ji-Wu-2, BEI  Xue-Jun-12, WU  Bo-12, YANG  Jia-Wei-3, ZHANG  Yong-Yan-12, JIANG  Bo-2, ZHU  Shi-Ping-1, YAN  Shu-Tang-1, ZHONG  Yun-2, ZHONG  Guang-Yan-2   

  1. 1.College of Horticulture and Landscape Architecture/Citrus Research Institute, Southwest University, Chongqing 400715
    2.Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640
    3.Economic Crops Station,  Agricultural Bureau of Rongxian, Zigong 643100, Sichuan
  • Received:2013-03-25 Online:2013-08-15 Published:2013-05-27

PDF

1980

Abstract: 【Objective】The objective of this study is to make clear the molecular mechanism and screen citrus tristeza virus (CTV) response genes in CTV susceptive citrus species ‘Jincheng’ orange [Citrus sinensis (L) Osbeck].【Method】 A suppression subtractive hybridization (SSH) library of ‘Jincheng’ orange was successfully constructed by using the virus-free leaves as Driver and those infected with CTV as Tester. 【Result】After sequencing of the randomly picked 850 clones, 742 clones were successfully sequenced and a total of 692 ESTs were obtained after Vectorscreen and adaptors deletion. All these ESTs were then matched to citrus genome transcripts and a total of 137 genes were identified. BLAST2GO gene ontology and KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis results showed that the basic energy metabolisms of ‘Jincheng’ orange were significantly influenced with differentially expressed genes (DEGs) involving in carbon fixation in photosynthetic organisms, nitrogen metabolism, starch and sucrose metabolism, etc. Moreover, DEGs largely involved in defense and resistance and some plant defensins synthesis related pathways such as carotenoid biosynthesis, phenylpropanoid biosynthesis, and phenylalanine metabolism. 【Conclusion】Results generated in this study suggested that CTV susceptible species ‘Jincheng’ orange reacted severely to CTV infection to maintain its growth and development by elevating energy metabolisms and enhancing the expression of stress resistance related genes.

Key words: citrus , suppression subtractive hybridization (SSH) , differentially expressed genes (DEGs) , CTV

[1]Zhou Y, Zhou C, Song Z, Liu K, Yang F. Characterization of citrus tristeza virus isolates by indicators and molecular biology methods. Agricultural Sciences in China, 2007, 6: 573-579.

[2]Zhou C Y, Hailstones D L, Broadbent P, Connor R, Bowyer J. Studies on mild strain cross protection against stem-pitting citrus tristeza virus. Fifteenth IOCV Conference, Citrus Tristeza Virus, 2002: 151-157.

[3]Diatchenko L, Lau Y F, Campbell A P, Chenchik A, Moqadam F, Huang B, Lukyanov S, Gurskaya N, Sverdlov E D, Siebert P D. Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proceedings of the National Academy of Sciences of the United States of America, 1996, 93(12): 6025-6030.

[4]Randall P J, Bouma D. Zinc deficiency, carbonic anhydrase, and photosynthesis in leaves of spinach. Plant Physiology, 1973, 52: 229-232.

[5]Karlsson J, Clarke a K, Chen Z Y, Hugghins S Y, Park Y I, Husic H D, MoroneyJ V, Samuelsson G. A novel alpha-type carbonic anhydrase associated with the thylakoid membrane in Chlamydomonas reinhardtii is required for growth at ambient CO2. The EMBO Journal, 1998, 17: 1208-1216.

[6]Gandía M, Conesa A, Ancillo G, Gadea J, Forment J, Pallás V, Flores R, Duran-Vila N, Moren P, Guerri J. Transcriptional response of Citrus aurantifolia to infection by citrus tristeza virus. Virology, 2007, 367: 298-306.

[7]Samanani N, Facchini P J. Purification and characterization of norcoclaurine synthase. The first committed enzyme in benzylisoquinoline alkaloid biosynthesis in plants. The Journal of Biological Chemistry, 2002, 277: 33878-33883.

[8]Samanani N, Liscombe D K, Facchini P J. Molecular cloning and characterization of norcoclaurine synthase, an enzyme catalyzing the first committed step in benzylisoquinoline alkaloid biosynthesis. The Plant Journal, 2004, 40: 302-313.

[9]Lee E J, Facchini P. Norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family. The Plant Cell, 2010, 22: 3489-3503.

[10]Bonas U, Lahaye T. Plant disease resistance triggered by pathogen- derived molecules: refined models of specific recognition. Current Opinion in Microbiology, 2002, 5: 44-50.

[11]Aarts N, Metz M, Holub E, Staskawicz B J, Daniels M J, Parker J E. Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 1998, 95: 10306-10311.

[12]De Hoff P L, Brill L M, Hirsch A M. Plant lectins: the ties that bind in root symbiosis and plant defense. Molecular Genetics and Genomics, 2009, 282: 1-15.

[13]Peumans W J, Van Damme E J. Lectins as plant defense proteins. Plant Physiology, 1995, 109: 347-352.

[14]Dowd C, Wilson I.W, McFadden H. Gene expression profile changes in cotton root and hypocotyl tissues in response to infection with Fusarium oxysporum f. sp. vasinfectum. Molecular Plant-Microbe Interactions, 2004, 17: 654-667.

[15]Sun H, Kim M K, Pulla R K, Kim Y J, Yang D C. Isolation and expression analysis of a novel major latex-like protein (MLP151) gene from Panax ginseng. Molecular Biology Reports, 2010, 37: 2215-2222.

[16]Lima L S, Gramacho K P, Carels N, Novais R, Gaiotto F A, Lopes U V, Gesteira A S, Zaidan H A, Cascardo J C M, Pires J L, Micheli F. Single nucleotide polymorphisms from Theobroma cacao expressed sequence tags associated with ‘witches’ broom disease in cacao. Genetics and Molecular Research, 2009, 8: 799-808.

[17]Harada E, Kim J-A, Meyer A J, Hell R, Clemens S, Choi Y E. Expression pro?ling of tobacco leaf trichomes identi?es genes for biotic and abiotic stresses. Plant Cell Physiology, 2010, 51(10): 1627-1637.

[18]Fan J, Gao X, Yang Y W, Deng W, Li Z G. Molecular cloning and characterization of a NAC-like gene in “Navel” orange fruit response to postharvest stresses. Plant Molecular Biology Reporter, 2007, 25: 145-153.

[19]Zhong R, Richardson E A, Ye Z H. Two NAC domain transcription factors, SND1 and NST1, function redundantly in regulation of secondary wall synthesis in fibers of Arabidopsis. Planta, 2007, 225: 1603-1611.

[20]Yoo S Y, Kim Y, Kim S Y, Lee J S, Ahn J H. Control of flowering time and cold response by a NAC-domain protein in Arabidopsis. PLOS one, 2007, 2: e642.

[21]Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J. A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science, 2006, 314: 1298-1301.

[22]Selth L A, Dogra SC, Rasheed M S, Healy H, Randles J W, Rezaian M A. A NAC domain protein interacts with tomato leaf curl virus replication accessory protein and enhances viral replication. The Plant Cell, 2005, 17: 311-325.

[23]Wu K, Rooney M F, Ferl R J. The Arabidopsis 14-3-3 multigene family. Plant Physiology, 1997, 114: 1421-1431.

[24]Delille J M, Sehnke P C, Ferl R J. The Arabidopsis 14-3-3 family of signaling regulators. Plant Physiology, 2001, 126: 35-38.

[25]Roberts M R, Bowles D J. Fusicoccin, 14-3-3 proteins, and defense responses in tomato plants. Plant Physiology, 1999, 119: 1243-1250.

[26]Lapointe G, Luckevich M D, Cloutier M, Séguin A. 14-3-3 gene family in hybrid poplar and its involvement in tree defence against athogens. Journal of Experimental Botany, 2001, 52: 1331-1338.

[27]Koo A J K, Cooke T F, Howe G. Cytochrome P450 CYP94B3 mediates catabolism and inactivation of the plant hormone jasmonoyl-L-isoleucine. Proceedings of the National Academy of Sciences of the United States of America, 2011, 108: 9298-9303.

[28]Rupasinghe S, Schuler M. Homology modeling of plant cytochrome P450s. Phytochemistry Reviews, 2006, 5: 473-505.

[29]Schuler M, Duan H, Bilgin M, Ali S. Arabidopsis cytochrome P450s through the looking glass: a window on plant biochemistry. Phytochemistry Reviews, 2006, 5: 205-237.

[30]Saito S, Hirai N, Matsumoto C, Ohigashi H, Ohta D. Arabidopsis CYP707As encode (+)-abscisic acid 8’-hydroxylase , a key enzyme in the oxidative catabolism of abscisic acid. Plant Physiology, 2004, 134: 1439-1449.

[31]Krochko J E, Abrams G D, Loewen M K, Abrams S R, Cutler A. (+) -Abscisic acid 8’ -hydroxylase is a cytochrome. Plant Physiology, 1998, 860: 849-860.

[32]Collazo P, Montoliu L, Puigdomènech P, Rigau J. Structure and expression of the lignin O-methyltransferase gene from Zea mays L. Plant Molecular Biology, 1992, 20: 857-867.

[33]Gowri G, Bugos R C, Campbell W H, Maxwell C A, Dixon R A, Division P B, Samuel T, Noble R, Box P O, Oklahoma G G. Stress responses in Alfalfa (Medicago sativa L.). Plant Physiology, 1991: 7-14.

[34]Maury S, Geoffroy P, and Legrand M. Tobacco O-methyltransferases involved in phenylpropanoid metabolism. The different caffeoyl- coenzyme A/5-hydroxyferuloyl-coenzyme A 3/5-O-methyltransferase and caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase classes have distinct substrate spec. Plant Physiology, 1999, 121: 215-224.

[35]Boerjan W, Ralph J, Baucher M. Lignin biosynthesis. Annual Review of Plant Biology, 2003, 54: 519-546.
[1] HAO Kun, CHEN HongDe, ZHANG Wei, ZHONG Yun, DANG MeiRong, ZHU ShiJiang, HUANG ZhiKun, JIN Ying. Comprehensive Evaluation of Water-Nitrogen Management Under Surge-Root Irrigation Based on Citrus Yield, Quality, and Water- Nitrogen Use Efficiency [J]. Scientia Agricultura Sinica, 2026, 59(4): 862-873.
[2] LIU Jie, HOU Rui, ZHOU ZeHua, YI TuYong. Antibacterial Activity of Polyhexamethylene Guanidine Against Xanthomonas citri pv. citri [J]. Scientia Agricultura Sinica, 2025, 58(9): 1779-1790.
[3] YAO Li, DENG ChunXiu, TANG Biao, WANG Hong, WU YueYing, ZHANG Qi, LI YuanHong, LIU ZhongYou, LU ChangAi, LIN ChaoWen. Suitability Evaluation of Late Maturing Citrus in Dongpo District, Meishan City, Sichuan Province Based on Maximum Entropy Model (MaxEnt) and Geographic Information System (GIS) [J]. Scientia Agricultura Sinica, 2025, 58(4): 748-758.
[4] QIN Lu, SHEN DanDan, JIANG XiaoLi, XIE HePing, AO YiJun, YANG Yang, ZHU Feng, XU RangWei, LIAO WenYue, CHENG YunJiang. Effect of Water Status on the Storability of Citrus Fruits Harvested Under Continuous Rainy Weather [J]. Scientia Agricultura Sinica, 2025, 58(24): 5259-5273.
[5] LI YaYu, WANG XinLiang, ZHOU JinHuan, LI ChuXin, LI JiaXin, TIAN XuBin, SONG Zhen. Construction and Infectivity Identification of Genome-Length cDNA of Citrus Psorosis Virus [J]. Scientia Agricultura Sinica, 2025, 58(17): 3451-3460.
[6] ZHANG SiNing, ZHANG XingRui, WU DongXuan, KANG JingBo, CHEN XiaoLin, GENG LiJun, YIN GuangMin, CHEN JiaJing, GAO JunYan, CAI ZhongHu, LIU Yuan, XU Juan. Aroma Quality Analysis of Guangdongxiangshui Lemon Based on Molecular Sensory Technology [J]. Scientia Agricultura Sinica, 2025, 58(1): 141-155.
[7] LI ChuXin, SONG ChenHu, ZHOU JinHuan, LI JiaXin, WANG XinLiang, TIAN XuBin, SONG Zhen. Research on Prevention and Control Technology of Citrus Yellow Vein Clearing Virus Based on VIGS [J]. Scientia Agricultura Sinica, 2024, 57(22): 4473-4482.
[8] WANG LuLu, ZHANG MingWei, YE JiaMin, ZHANG RuiFen, DENG Mei. Effects of Soluble and Insoluble Dietary Fiber from Shatianyu Pulp on Gut Microbiota [J]. Scientia Agricultura Sinica, 2024, 57(20): 4119-4129.
[9] WANG XianDa, ZHANG LiJie, WU XingMing, CHENG DeMing, LI Jian. Occurrence Rules of Citrus Fruit Shape and Peel Cracks [J]. Scientia Agricultura Sinica, 2024, 57(17): 3458-3468.
[10] CAO Peng, ZHOU JinHuan, WANG XinLiang, LI ChuXin, LI JiaXIN, JIANG Pei, LIU JinXiang, SONG Zhen. Optimization and Application of Rapid Evaluation System for Citrus Huanglongbing Resistance Mediated by Agrobacterium rhizogenes [J]. Scientia Agricultura Sinica, 2024, 57(16): 3182-3191.
[11] YE JiaMin, ZHANG MingWei, LU Qi, ZHANG RuiFen, DENG Mei. Effects of Semi-Solid Fermentation with Lactobacillus on the Bitterness and Active Components of Shatianyu (Citrus grandis L. Osbeck) Fruit Powder [J]. Scientia Agricultura Sinica, 2024, 57(13): 2662-2673.
[12] WANG ZhaoHao, GUO XingRu, ZHANG LeHuan, HE YongRui, CHEN ShanChun, YAO LiXiao. Expression Pattern of csi-miR399 in Response to Xanthomonas citri subsp. citri Infection and Its Disease Resistance Analysis [J]. Scientia Agricultura Sinica, 2023, 56(8): 1484-1493.
[13] YAN PeiHan, LUO JianMing, HAO ChenXing, SUN ZiQing, YE RongChun, LI Yi, LIU Lian, SHENG Ling, MA XianFeng, DENG ZiNiu. Identification of the Transcription Factor WRKY75 of CmPR4A in Citron C-05 and Its Function Analysis in Resistance to Citrus Canker Disease [J]. Scientia Agricultura Sinica, 2023, 56(21): 4304-4317.
[14] LU YanQing, LIN YanJin, WANG XianDa, LU XinKun. A Transcriptome Analysis Identifies Candidate Genes Related to Fruit Cracking in Pomelo Fruits [J]. Scientia Agricultura Sinica, 2023, 56(20): 4087-4101.
[15] LI FeiFei, LIAN XueFei, YIN Tao, CHANG YuanYuan, JIN Yan, MA XiaoChuan, CHEN YueWen, YE Li, LI YunSong, LU XiaoPeng. The Relationship Between Mastication and Development of Segment Membranes in Citrus Fruits [J]. Scientia Agricultura Sinica, 2023, 56(2): 333-344.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd