Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (9): 1946-1955.doi: 10.3864/j.issn.0578-1752.2013.09.023

• RESEARCH NOTES • Previous Articles     Next Articles

Expression Profiling of Cotton (Gossypium hirsutum L.) Trihelix Genes Responsive to Abiotic Stresses

 LI  Yue, SUN  Jie, XIE  Zong-Ming, LI  Quan-Sheng, SI  Ai-Jun, CHEN  Shou-Yi   

  1. 1.Agricultural College, Shihezi University/The Key Oasis Eco-Agriculture Laboratory of Xinjiang Production and Construction Group, Shihezi 832003, Xinjiang
    2.Center for Molecular Agrobiotechnology and Breeding, Xinjiang Academy of Agricultural and Reclamation Science/Xinjiang Production and Construction Group Key Laboratory of Crop Germplasm Enhancement and Gene Resources Utilization, Shihezi 832000, Xinjiang
    3.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences/National Key Laboratory of Plant Genomics, Beijing 100101
  • Received:2012-12-04 Online:2013-05-01 Published:2013-03-28

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Abstract: 【Objective】The objective of this study is to dissect cotton trihelix genes expression alteration responsive to different abiotic stresses, thus providing a foundation for stress-related genes cloning and elucidating the molecular mechanism of cotton stress-resistance.【Method】Semi-quantitative RT-PCR and real-time PCR were employed to reveal the expression profiling of 24 GhGTs under different abiotic stresses (200 mmol.L-1 NaCl, drought, 4℃) and exogenous hormone (100 μmol.L-1ABA) treatments.【Result】The results showed that the expression of 12 GhGT genes were up- and down-regulated by salinity and drought stresses, and that of 7 GhGT genes and 9 GhGT genes were up- and down-regulated by cold and ABA treatments, respectively. Moreover, three genes including GhGT2, GhGT18 and GhGT23 were response to all above treatments. 【Conclusion】The results suggested that GhGTs may play important roles in upland cotton plant abiotic stress adaption.

Key words: cotton , trihelix transcription factor , abiotic stress , expression

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