Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (1): 146-153.doi: 10.3864/j.issn.0578-1752.2013.01.017

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

MicroRNAs in Ovaries of Goats (Capra hircus) Identified by Solexa Sequencing

 ZHANG  Xiao-Dong, LING  Ying-Hui, ZHANG  Yun-Hai, LI  Yun-Sheng, LIU  Ya, CAO  Hong-Guo, ZHANG  Zi-Jun, YIN  Zong-Jun, DING  Jian-Ping, ZHANG  Xiao-Rong   

  1. College of Animal Science and Technology, Anhui Agricultural University/Anhui Key Laboratory of Local Animal Genetic Resources Conservation and Biobreeding, Hefei 230036
  • Received:2012-06-26 Online:2013-01-01 Published:2012-07-27

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Abstract: 【Objective】 Characterization of microRNA expression in hircine ovary tissues was studied, with the purposes of providing basic information for further studies of relationships between specific miRNAs and hircine reproduction such as folliculogenesis and sex hormone secretion. 【Method】 The small RNAs isolated from total RNA of ovary tissues were sequenced by Solexa and then bioinformatics analysis were perfomed. Meanwhile, the expression of selected miRNAs was validated by q-PCR. 【Result】Sequence analysis indicated that 508 ovary miRNAs and 19 corresponding miRNA*s were identified which are conservative in evolution of mammals (sheep, bovine, swine, horse, dog). The expression of 8 selected miRNAs in ovary tissues obtained by q-PCR was in agreement with Solexa sequencing results. 【Conclusion】 The expression profiling of miRNAs, which are abundant and differentially expressed in hircine ovary tissues, was constructed successfully.

Key words: miRNA , Solexa , ovary , Anhui White goat

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