Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (21): 4475-4483.doi: 10.3864/j.issn.0578-1752.2012.21.016

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Characterization of Tissue Expression and Cloning of Nuclear Factor I/B Gene in Chinese Merino (Xinjiang Junken Type)

 RONG  恩Guang, YU  Lei, YANG  Hua, ZHANG  Yong-Sheng, MA  Chun-Ping, LI  Hui, WANG  Ning   

  1. 1.东北农业大学动物科学技术学院，哈尔滨 150030
    2.新疆农垦科学院畜牧兽医研究所，新疆石河子 832000
    3.新疆西部牧业股份有限责任公司，新疆石河子 832000
  • Received:2012-03-05 Online:2012-11-01 Published:2012-05-18

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Abstract: 【Objective】The objective of this study was to explore the expression level of nuclear factor I/B gene in various sheep tissues, and clone the full-length coding sequence (CDS) of sheep NFIB gene in Chinese Merino (Xinjiang Junken type).【Method】Tissue expression profile of NFIB gene was detected by semi-quantitative reverse transcription PCR (SqRT-PCR). The full-length CDS region of NFIB gene was amplified by RT-PCR, cloned into T vector, and sequenced. The sequence was analyzed by using bioinformatics software.【Result】SqRT-PCR analysis showed that the NFIB gene was ubiquitously expressed in sheep internal organs and skin with different degrees. Comparatively, it was highly expressed in skin. The skin expression of NFIB gene in super fine wool strain had significant difference in different seasons (P＜0.05), but no significant difference in skins from various anatomic locations. There was no significant difference in the NFIB gene expression of body side skin among the 6 sheep strains/breeds (P＞0.05). Sequence analysis indicated that the sheep NFIB gene generated three NFIB protein isoforms of 420, 375 and 345 aa by alternative splicing.【Conclusion】NFIB gene is highly expressed in the skin of Chinese Merino (Xinjiang Junken type), and its expression in the skin of super fine wool strain has significant difference in different seasons. Sheep NFIB has at least three protein isoforms. This study not only has laid a foundation for clarifying the function of sheep NFIB gene, but also provided a theoretical basis for creating high quality merino sheep.

Key words: NFIB gene, gene expression, Chinese merino, gene cloning, protein isoform, super fine wool strain

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