Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (19): 3988-3995.doi: 10.3864/j.issn.0578-1752.2012.19.010

• PLANT PROTECTION • Previous Articles     Next Articles

Effects of Bemisia tabaci (Homoptera: Aleyrodidae) Biotype B Infestation on Photosystem II in Nicotiana tabacum

 LI  Qing-Liang, TAN  Wei, XUE  Ming   

  1. 1.山东农业大学植物保护学院，山东泰安 271018
    2.山东农业大学园艺科学与工程学院/作物生物学国家重点实验室，山东泰安 271018
  • Received:2012-01-06 Online:2012-10-01 Published:2012-04-20

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Abstract: 【Objective】The objective of this study is to define the effects of Bemisia tabaci biotype B infestation on the photosystem II (PSII) in tobacco, and to reveal the mechanism of B. tabaci infestation affecting photosynthesis of host plant. 【Method】Effects of B. tabaci infestation on the PSII performance in damaged leaves and systemic leaves of tobacco were investigated by measuring chlorophyll fluorescence transients and analyzing the related parameters using JIP-test.【Result】B. tabaci infestation significantly increased the maximal photochemical efficiency of primary photochemistry (φpo) and performance index on absorption basis (PIABS) both in local and systemic leaves (P＜0.05), indicating that the original photochemical reaction was greatly damaged. Photosynthetic electron transport was inhibited and oxygen-evolving complexes (OEC) were severely damaged. B. tabaci infestation increased the energy absorption per active reaction centers (ABS/RC) and the energy dissipation per active reaction centers (DIo/RC) increase while decreased energy trapping per active reaction centers (TRo/RC) in systemic leaves. In damaged leaves, DIo/RC increased and TRo/RC decreased, however, the ABC/RC was not affected. B. tabaci infestation reduced the density of active reaction centers per excited cross-section (RC/CS) both in damaged and systemic leaves (P＜0.05) while increased the closure degree of the PSⅡreaction centers by 69.83% and 142.58% (P＜0.05), respectively, in damaged and systemic leaves.【Conclusion】B. tabaci infestation damaged the PSII of tobacco leaves. The reaction centers were damaged and the electron transporting was inhibited. The inhibited points of the electron transporting included the OEC and the electron transporting from QA to QB. Energy flux of PSII was also affected by the B. tabaci infestation. The damage on PSII caused by B. tabaci in tobacco leaves was systematically conductive.

Key words: tobacco, Bemisia tabaci biotype B, photosystem II, oxygen-evolving complexes, electron transporting

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