玉米大斑病菌转录因子StSte12的活性及其对酵母生长的功能互补分析

doi:10.3864/j.issn.0578-1752.2012.16.007

Abstract

Abstract: 【Objective】 The objective of this study is to identify the structure characteristics, transcription activity and function of StSte12 from Setosphaeria turcica. 【Method】Conservative domain prediction and phylogenetic tree analysis were used to predict the possible function of StSte12 through bioinformatics analysis. The transcription activity of StSTE12 was tested by β-galactosidase activity method. Through characterization of growth of the StSTE12 complement transformant, in which StSTE12 was introduced into ScSTE12 null mutant of Saccharomyces ceresivisiae, the function of StSTE12 was preliminarily identified.【Result】StSte12 contained STE homeodomain and ZnF_C2H2¬ structure, which was the characteristics of Ste12-like transcription factor. Sequence analysis revealed that StSTE12 shared highly homology with other STE12-like genes of plant pathogens. StSte12 had transcription activation in vitro and could restore the function of ste12Δ of S. ceresivisiae, which regulated invasion growth of the yeast cell. 【Conclusion】Transcription factor StSTE12, a STE12-like gene from S. turcica, has a transcription activity and plays an important role in regulating the invasion growth of the cell on YPD medium.

Key words: Setosphaeria turcica, transcription factor, StSTE12, invasion growth

WU Min, GU Shou-Qin, LI Po, WANG Mei-Juan, ZHANG Chang-Zhi, FAN Yong-Shan, HAN Jian-Min, DONG Jin-Gao. Transcription Activity of a Transcription Factor StSte12 from Setosphaeria turcica and Function Analysis Through Yeast Complementation[J].Scientia Agricultura Sinica, 2012, 45(16): 3281-3287.

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Transcription Activity of a Transcription Factor StSte12 from Setosphaeria turcica and Function Analysis Through Yeast Complementation

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