谷子穗顶端败育突变体sipaa1的表型分析和基因定位

doi:10.3864/j.issn.0578-1752.2018.09.002

Abstract

Abstract: 【Objective】Panicle development determines crop yield, and panicle apical abortion is one of the major limitation that affects the grain yield of foxtail millet. Our study explored the genes and biological pathways related to panicle apical abortion in foxtail millet, which provided a theoretical basis for the genetic mechanism of foxtail millet panicle development.【Method】A foxtail millet panicle apical abortion mutant sipaa1, induced from Yugu1 by EMS treatment, was genetically identified. Agronomic traits of the mutant were investigated. The F₂ segregating population of sipaa1× SSR41 was used for gene mapping. Based on differential expressed levels of candidate genes in five different tissues of foxtail millet, some genes highly expressed in the panicle was identified. Transcriptome sequencing of Yugu1 and sipaa1 young panicle at booting stage was conducted to find differential expressed genes and to analyze the biological pathways.【Result】The mutant exhibited thinner panicle, shorter leaf, less spikelets per panicle, lower spikelet numbers, as well as lower 1000-grain weight, compared with wildtype Yugu1. The agronomic traits of mutants showed that mutant leaf length, leaf width, panicle length, panicle diameter, panicle weight per plant, spikelet number per panicle, grain weight per spikelet and 1000-grain weight were decreased 10.66%, 5.08%, 11.36%, 16.12%, 30.02%, 32.58%, 30.55%, 18.18%, respectively. Genetic analysis showed that segregating ratio of wild type to mutant plants were 3:1 in sipaa1×SSR41 F₂ generation, suggesting that the panicle apical abortion trait of sipaa1 was controlled by a single recessive nuclear gene. By map-based cloning, the candidate gene was mapped into a region between Indel markers In1-9.23 and In1-9.333 in chromosome 1, which contains a 100 kb interval. Combined with transcriptome sequencing, some candidate genes were found. Six candidate genes highly expressed in the panicle were further identified. Moreover, transcriptome sequencing showed that there were 2 768 up-regulated genes and 507 down-regulated genes between mutant and wild type. The differential expressed genes were mainly enriched in hormone signal transduction, external stress responses, plant-pathogen interaction.【Conclusion】Casual gene was limited into a 100 Kb region between Indel markers 1-9.23 and 1-9.333 on chromosome 1 of foxtail millet. Combined transcriptome sequencing and gene function analysis, we identified two candidate genes that are highly expressed in panicle, and reported to participate in plant floral development and stress responses. Panicle apical abortion may be regulated by hormone, stress responses, and programmed cell death pathway.

Key words: Setaria italica, panicle apical abortion, phenotypic analysis, gene mapping, RNAseq

XUE HongLi, YANG JunJun, TANG Sha, ZHI Hui, WANG Rui, JIA GuanQing, QIAO ZhiJun, DIAO XianMin. Morphological Characterization and Gene Mapping of a Panicle Apical Abortion Mutant (sipaa1) in Foxtail Millet[J].Scientia Agricultura Sinica, 2018, 51(9): 1627-1640.

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Morphological Characterization and Gene Mapping of a Panicle Apical Abortion Mutant (sipaa1) in Foxtail Millet

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