Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (11): 2267-2272.doi: 10.3864/j.issn.0578-1752.2012.11.016

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Dynamic Expression of TPM1 Gene During Longissimus dorsi Muscle Development in Pigs

 LIANG  Ru-Yi, LI  Yan, TANG  Zhong-Lin, HUANG  Rui-Hua, LI  Kui   

  1. 1.南京农业大学动物科技学院，南京210095
    2.中国农业科学院北京畜牧兽医研究所/农业部畜禽遗传资源与利用重点开放实验室，北京 100193
  • Received:2011-12-29 Online:2012-06-01 Published:2012-03-12

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Abstract: 【Objective】 The purpose of the present research was to explore whether tropomyosin (TM) has biological functions in the regulation of the Longissimus dorsi muscle growth and development of pigs.【Method】In this study, TPM1 gene expression profiles in different tissues from Tongcheng pigs and Landrace pigs were analyzed and the expression change during Longissimus dorsi muscle development (28 stages) was investigated by real-time qPCR with SYBR® Green II. 【Result】The result showed that the TPM1 gene was strongly expressed in the Longissimus dorsi muscle and heart, and both breeds were similar in the expression patterns with decreasing after increasing, but the peak occurred at different times. The expression level of TPM1 gene in embryonic period 75 d were significantly higher than those in other periods in Tongcheng pigs, and the same result was found in embryonic period 90 d in Landrace pigs.【Conclusion】Results suggested that TPM1 gene expression may be related to the Longissimus dorsi muscle growth and muscle phenotypic differences between species of pigs.

Key words: TPM1 gene, RT-qPCR, Longissimus dorsi muscle development, dynamic expression, pig

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