Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (11): 2260-2266.doi: 10.3864/j.issn.0578-1752.2012.11.015

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Stability of Endogenous Reference Genes in Green-Goose Tissues During Prelaying and Laying Periods 

 JI  Hong, WANG  Zhong-Wei, GUO  Jing-Ru, WANG  Jian-Fa, HE  Rong-He, YANG  Huan-Min   

  1. 1.黑龙江八一农垦大学动物科技学院，黑龙江大庆163319
    2.吉林大学畜牧兽医学院，长春130062
  • Received:2011-06-08 Online:2012-06-01 Published:2011-08-30

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Abstract: 【Objective】 The mRNA expression levels of seven candidate reference genes in green-goose tissues during prelaying and laying periods were comparatively-analyzed. Finally the most appropriate reference genes and their numbers were screened in gene expression analysis of green-goose tissues.【Method】The expressions of candidate reference genes 28S rRNA, 18S rRNA, GAPDH, ACTB, HPRT1, SDH and TUB in prelaying and laying green-goose liver, kidney, heart, leg muscle and ovary were detected by qRT-PCR. Gene copy number was determined using absolute quantification. Then the expression stability of these genes was analysed by geNorm software.【Result】The results showed that stability of the seven reference genes from high to low was GAPDH=HRPT1 (0.195)＞TUB (0.244)＞28S rRNA (0.414)＞18S rRNA (0.495)＞ACTB (0.541)＞SDH (0.610) during prelaying period and GAPDH=28S rRNA (0.128)＞TUB (0.181)＞ACTB (0.192)＞SDH (0.221)＞HRPT1 (0.316)＞18S rRNA (0.362) during laying period by geNorm analysis. And V2/3 of pairwise variance analysis of 7 candidate reference genes were separately 0.084 and 0.069 during the 2 periods. The appropriate number of reference genes was 2. 【Conclusion】 These results suggest that GAPDH and HRPT1, GAPDH and 28S rRNA are suitable reference genes and could be used to normalize mRNA levels between different samples in green-goose tissues during prelaying and laying periods.

Key words: green-goose, reference gene, qRT-PCR, geNorm software, NormFinder software

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