Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (9): 1864-1872.doi: 10.3864/j.issn.0578-1752.2012.09.023

• RESEARCH NOTES • Previous Articles     Next Articles

Effects of Exogenous Nitric Oxide on Main Growth and Physiological Characteristics of Seedlings of Cotton Under NaCl Stress

 LI  Cui-Fang, LIU  Lian-Tao, SUN  Hong-Chun, ZHANG  Yong-Jiang, ZHU  Xiu-Jin, LI  Cun-Dong   

  1. 1.河北农业大学农学院/河北省作物生长调控重点实验室，河北保定 071000
    2.河北省南宫市气象局，河北南宫 055750
  • Received:2011-12-05 Online:2012-05-01 Published:2012-02-10

PDF

7449

Cited

4

Abstract: 【Objective】 The purpose of this study is to clarify the effects of nitric oxide (NO) on cotton seedlings growth and leaf physiological characteristics under NaCl stress. 【Method】 In a hydroponic experiment, two NaCl concentrations (50 mmol•L-1 and 100 mmol•L-1), four sodium nitroprusside (SNP) concentrations (0, 50, 100 and 200 μmol•L-1) for each NaCl level were designed, and at the same time a conventional culture was set. The growth and leaves physiological characteristics of cotton seedlings were measured after 10 days of treatment.【Result】 The results showed that the treatment of NaCl stress inhibited the growth of cotton seedlings. The internodes of the cotton seedlings under NaCl stress were inhibited further by SNP which is an exogenous nitric oxide donor. The relative water content of cotton seedlings, especially the relative water content of the stem and root were decreased in SNP treatments under NaCl stress. The treatments of SNP added in 100 mmol•L-1 NaCl stress increased the contents of soluble protein and chlorophyll, and the ratio of chlorophyll a/b, the effects of 50 μmol•L-1 SNP was the best. Under both of the NaCl stresses, SNP improved the activities of SOD and POD significantly, while the activities of CAT decreased.【Conclusion】 Under this experimental condition, exogenous nitric oxide inhibited internodes elongated, alleviated chlorophyll degradation, enhanced antioxidant capacity and improved leaves quality of cotton seedlings under NaCl stress. The SNP concentration from 50 μmol•L-1 to 100 μmol•L-1 could alleviate NaCl stress for cotton seedlings, the cotton seedlings under NaCl stress were seriously injured by high concentrations (200 μmol•L-1）of SNP.

Key words: cotton seedlings, nitric oxide, NaCl stress, seedlings growth, physiological characteristics

[1]辛承松, 董合忠, 孔祥强, 罗  振. 棉花不同类型品种苗期耐盐性差异研究. 中国农学通报, 2011, 27(05): 180-185.

Xin C S, Dong H Z, Kong X Q, Luo Z. Salt tolerant variation between different type cotton cultivars at seedling stage. Chinese Agricultural Science Bulletin, 2011, 27(05): 180-185. (in Chinese)

[2]董合忠, 王留明, 赵洪亮, 周建国. 中国棉业科技进步30年——山东篇. 中国棉花. 2009, 36(增刊): 12-17.

Dong H Z, Wang L M, Zhao H L, Zhou J G. China’s cotton industry science and technology progress 30 years-shandong. China Cotton, 2009, 36(suppl.): 12-17. (in Chinese)

[3]孙小芳, 刘友良, 陈  沁. 棉花耐盐性研究进展. 棉花学报, 1998, 10(3): 118-124.

Sun X F, Liu Y L, Chen Q. Recent progresses in studies on salinity tolerance in cotton. Acta Gossypii Sinica, 1998, 10(3): 118-124. (in Chinese)

[4]Delledonne M, Xia Y, Dixon R A, Lamb C. Nitric oxide functions as a signal in plant disease resistance. Nature, 1998, 394:585-588.

[5]Durner J, Wendehemme D, Klessig D F. Defense gene induction in tobacco by nitric oxide, cyclic GMP and cyclic ADP-ribose. Proceedings of the National Academy of Sciences of USA, 1998, 95: 10328-10333.

[6]Durner J, Klessig D F. Nitric oxide as a signal in plants. Current Opinion in Plant Biology, 1999, 2: 369-374.

[7]Beligni M V, Lamattina L. Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants. Planta, 2000, 210: 215-221.

[8]黄忠勤, 杨  峰, 樊继德, 周兴根, 王基华, 熊  伟. 一氧化氮对汞胁迫下小麦种子萌发及抗氧化代谢的影响. 安徽农业科学, 2011, 39(9): 5161-5163.

Huang Z Q, Yang F, Fan J D, Zhou X G, Wang J H, Xiong W. Effects of nitric oxide on germination and antioxidant of wheat seeds under Hg2+ stress. Journal of Anhui Agricultural Science, 2011, 39(9): 5161-5163. (in Chinese)

[9]肖  强, 陈  娟, 吴飞华, 郑海雷. 外源NO供体硝普钠（SNP）对盐胁迫下水稻幼苗中叶绿素和游离脯氨酸含量及抗氧化酶的影响. 作物学报,2008, 34(10): 1849-1853.

Xiao Q, Chen J, Wu F H, Zheng H L. Effects of exogenous nitric oxide donor SNP on contents of chlorophyll and free proline, activity of antioxidative enzyme in rice seedlings under NaCl stress. Acta Agronomica Sinica, 2008, 34(10): 1849-1853. (in Chinese)

[10]刘开力, 凌腾芳, 刘志兵, 花  榕, 孙永刚, 沈文彪. 外源NO供体SNP浸种对盐胁迫下水稻幼苗生长的影响. 植物生理学通讯, 2004, 40(4): 419-422.

Liu K L, Ling T F, Liu Z B, Hua R, Sun Y G, Shen W B. Effects of soaking seeds in exogenous nitric oxide donor on growth of rice seedlings under salt stress. Plant Physiology Communications, 2004, 40(4): 419-422. (in Chinese)

[11]刘建新, 胡浩斌, 王  鑫. 外源NO对盐胁迫下黑麦草幼苗根生长抑制和氧化损伤的缓解效应. 植物研究, 2008, 28(1):7-12.

Liu J X, Hu H B, Wang X. Alleviative effects of exogenous nitric oxide on root growth inhibition and its oxidative damage in rye grass seedlings under NaCl stress. Bulletin of Botanical Research, 2008, 28(1): 7-12. (in Chinese)

[12]阮海华, 沈文飚, 叶茂炳, 徐朗莱. 一氧化氮对盐胁迫下小麦叶片氧化损伤的保护效应. 科学通报, 2001, 46(23): 1993-1997.

Ruan H H, Shen W B, Ye M B, Xu L L. Protection of nitric oxide on salt-induced membrane oxidation in whewt leaves. Bulletin of Science, 2001, 46(23): 1993-1997. (in Chinese)

[13]孙  锋. 一氧化氮和亚甲基蓝对盐胁迫抗虫棉根系的抗氧化能力的影响. 棉花学报, 2009, 21(4): 313-318.

Sun F. Effects of nitric oxide and methylene blue on antioxidant capacity of the insect-resistant cotton seedlings roots under NaCl stress. Cotton Science, 2009, 21(4): 313-318. (in Chinese)

[14]孟艳艳, 范术丽, 宋美珍, 庞朝友, 喻树迅. NO对生长发育中棉花叶片NO含量及其对抗氧化物酶的影响. 作物学报, 2011, 37(10): 1828-1836.

Meng Y Y, Fan S L, Song M Z, Pang C Y, Yu S X. Effects of NO on NO contents and anti-oxidative enzymes in cotton leaf at growth stage. Acta Agronomtca Sinica, 2011, 37(10): 1828-1836. (in Chinese)

[15]邹  琦. 植物生理学实验指导. 北京: 中国农业出版社, 2000.

Zou Q. Experimental Guidance for Plant Physiology. Beijing: Chinese Agriculture Press, 2000. (in Chinese)

[16]Read S M, Northcote D H. Minimization of variation in the response to different protein of the coomassic blue G dye dinding: assay for protein. Analytical Biochemistry, 1981, 116: 53-64.

[17]华东师范大学. 植物生理学实验指导. 北京: 人民教育出版社, 1980.

East China Normal University. Experimental Guidance for Plant Physiology. Beijing: People’s Education Press, 1980. (in Chinese)

[18]刘金定, 朱召勇, 樊宝香. 棉花品种在不同浓度盐胁迫下的生理表现. 中国棉花, 1995, 22(9): 16-17.

Liu J D, Zhu Z Y, Fan B X. The expression physiological characteristics of different cotton cultivars under different concentration NaCl stress. China Cotton, 1995, 22(9): 16-17. (in Chinese)

[19]马  丽, 侯振安, 梁永超, 龚  江, 胡  征, 周小丽, 许化友. NaCl胁迫对棉花幼苗生理特性的影响, 石河子大学学报:自然科学版, 2008, 26(2): 180-184.

Ma L, Hou Z A, Liang Y C, Gong J, Hu Z, Zhou X L, Xu H Y. Effects of NaCl stress on physiological characteristics of different cotton cultivars in seedlings stage. Journal of Shihezi University:Natural Science, 2008, 26(2): 180-184. (in Chinese)

[20]辛承松, 唐  薇, 王洪征, 翟志席. 鲁棉14幼苗生长对氯化钠胁迫的反应及微量元素、激素处理的效应. 棉花学报, 2002, 14(2): 108-112.

Xin C S, Tang W, Wang H Z, Zhai Z X. Responses of seedling growth of Lumian 14 to NaCl stress and effects of treatments with microelement and hormone. Cotton Science, 2002, 14(2): 108-112. (in Chinese)

[21]吴雪霞, 朱月林, 朱为民, 陈建林, 刘正鲁. 外源一氧化氮对NaCl胁迫下番茄幼苗生理影响. 中国农业科学, 2006, 39(3): 575-581.

Wu X X, Zhu Y L, Zhu W M, Chen J L, Liu Z L. Physiological effects of exogenous nitric oxide in tomato seedlings under NaCl stress. Scientia Agricultura Sinica, 2006, 39(3): 575-581. (in Chinese)

[22]吴雪霞, 陈建林, 查丁石, 朱为民. 外源一氧化氮对NaCl胁迫下番茄幼苗活性氧代谢的影响. 植物营养与肥料学报, 2009, 15(2): 422-428.

Wu X X, Chen J L, Zha D S, Zhu W M. Effects of exogenous nitric on reactive oxygen metabolism in tomato seedlings under NaCl stress. Plant Nutrition and Fertilizer Science, 2009, 15(2): 422-428. (in Chinese)

[23]张艳艳, 刘  俊, 刘友良. 一氧化氮缓解盐胁迫对玉米生长的抑制作用. 植物生理与分子生物学学报, 2004, 30(4): 455-459.

Zhang Y Y, Liu J, Liu Y L. Nitric oxide alleviates growth inhibition of maize seedlings under NaCl stress. Journal of Plant Physiology and Molecular Biology, 2004, 30(4): 455-459. (in Chinese)

[24]郑春芳, 姜  东, 戴廷波, 荆  奇, 曹卫星. 外源一氧化氮供体硝普钠浸种对盐胁迫下小麦幼苗碳氮代谢及抗氧化系统的影响. 生态学报, 2010, 30(5): 1174-1183.

Zheng C F, Jiang D, Dai T B, Jing Q, Cao W X. Effects nitroprusside, a nitric oxide donor, on carbon and nitrogen metabolism and the activity of the anti-oxidation system in wheat seedlings under salt stress. Acta Ecologica Sinica, 2010, 30(5): 1174-1183. (in Chinese)

[25]汤绍虎, 周启贵, 孙  敏, 毛  薇. 外源NO对渗透胁迫下黄瓜种子萌发、幼苗生长和生理特性的影响. 中国农业科学, 2007, 40(2): 419-425.

Tang S H, Zhou Q G, Sun M, Mao W. Effects of exogenous nitric oxide on seed germination, seedling growth and physiological characteristics of cucumber under osmotic pressure. Scientia Agricultura Sinica, 2007, 40(2): 419-425. (in Chinese)
[1] XU Ke,FAN ZhiLong,YIN Wen,ZHAO Cai,YU AiZhong,HU FaLong,CHAI Qiang. Coupling Effects of N-fertilizer Postponing Application and Intercropping on Maize Photosynthetic Physiological Characteristics [J]. Scientia Agricultura Sinica, 2022, 55(21): 4131-4143.
[2] ZHANG LongYan,CHENG GongMin,WEI HengLing,WANG HanTao,LU JianHua,MA ZhiYing,YU ShuXun. Chilling Tolerance Identification and Response to Cold Stress of Gossypium hirsutum Varieties (Lines) During Germination Stage [J]. Scientia Agricultura Sinica, 2021, 54(1): 19-33.
[3] LI XueRu,SHI XiXiong,WANG JianZhong,ZHANG PanGao,TIAN Zhu,HAN Ling. Effect of Nitric Oxide Synthetase Inhibitor on Yak Meat Quality During Post-Mortem Aging [J]. Scientia Agricultura Sinica, 2020, 53(8): 1617-1626.
[4] YUAN YuHao, YANG QingHua, DANG Ke, YANG Pu, GAO JinFeng, GAO XiaoLi, WANG PengKe, LU Ping, LIU MinXuan, FENG BaiLi. Salt-Tolerance Evaluation and Physiological Response of Salt Stress of Broomcorn Millet (Panicum miliaceum L.) [J]. Scientia Agricultura Sinica, 2019, 52(22): 4066-4078.
[5] YIN MeiQiang,WANG Dong,WANG JinRong,LAN Min,ZHAO Juan,DONG ShuQi,SONG Xi’E,ALAM Sher,YUAN XiangYang,WANG YuGuo,WEN YinYuan. Effects of Exogenous Nitric Oxide on Seed Germination and Starch Transformation of Sorghum Seeds Under Salt Stress [J]. Scientia Agricultura Sinica, 2019, 52(22): 4119-4128.
[6] BIAN FengE, XIAO QiuHong, HAO GuiMei, SUN YongJiang, LU WenLi, DU YuanPeng, ZHAI Heng. Effect of Root-Applied Melatonin on Endogenous Melatonin and Chlorophyll Fluorescence Characteristics in Grapevine Under NaCl Stress [J]. Scientia Agricultura Sinica, 2018, 51(5): 952-963.
[7] HUANG Ping, CAO Hui, ZHANG RuiXue, JI Tuo, LI YanGe, YANG HongQiang. Different Response of Apple Root Physiology and Leaf Photosynthesis to Mulching of Different Materials [J]. Scientia Agricultura Sinica, 2018, 51(1): 160-169.
[8] WU Yang, GAO HuiChun, ZHANG BiXian, ZHANG HaiLing, WANG QuanWei, LIU XinLei, LUAN XiaoYan, MA YanSong. Effects of 24-Brassinolide on the Fertility, Physiological Characteristics and Cell Ultra-Structure of Soybean Under Saline-Alkali Stress [J]. Scientia Agricultura Sinica, 2017, 50(5): 811-821.
[9] WANG Hui, ZHANG HaiJuan, LI ZhiDong. Relaxed Effect of Caulis Lonicerae Extraction on Contraction of Intestinal Smooth Muscle of Rabbit in Vitro and Its Mechanism [J]. Scientia Agricultura Sinica, 2017, 50(2): 372-379.
[10] SHAO Rui-xin, LI Lei-lei, ZHENG Hui-fang, XIN Long-fei, SU Xiao-yu, RAN Wu-ling, YANG Qing-hua. Effects of Exogenous Nitric Oxide on Photosynthesis of Maize Seedlings Under Drought Stress [J]. Scientia Agricultura Sinica, 2016, 49(2): 251-259.
[11] CAI Chong, XU Ying-ying, CUI Xu-hong. Analysis of Physiological Characteristics with Response to Bemisia tabaci B Biotype in Different Resistant Varieties of Tomato [J]. Scientia Agricultura Sinica, 2016, 49(13): 2524-2533.
[12] FAN Wei-guo, LUO Yan. Growth Status, Root Morphology andPhysiologicalCharacteristics of Four Citrus Rootstocks Under Different Phosphorus Levels [J]. Scientia Agricultura Sinica, 2015, 48(3): 534-545.
[13] LI Xiu, GONG Biao, WANG Yun, XU Kun. Heat Stress Mitigation by Exogenous Nitric Oxide Application Involves Polyamine Metabolism and PSII Physiological Strategies in Ginger Leaves [J]. Scientia Agricultura Sinica, 2014, 47(6): 1171-1179.
[14] YANG Ning-Ning-1, 3 , SUN Wan-Cang-2, 3 , LIU Zi-Gang-1, 2 , 3 , SHI Peng-Hui-1, 3 , FANG Yan-1, 2 , 3 , WU Jun-Yan-2, 3 , ZENG Xiu-Cun-1, 3 , KONG De-Jing-1, 3 , LU Mei-Hong-1, 3 , WANG Yue-1, 3 . Morphological Characters and Physiological Mechanisms of Cold Resistance of Winter Rapeseed in Northern China [J]. Scientia Agricultura Sinica, 2014, 47(3): 452-461.
[15] CHEN Jing, LIU Lian-tao, SUN Hong-chun, ZHANG Yong-jiang, WANG Zhan-biao, LI Cun-dong. Regulatory Effects of Exogenous Nitric Oxide on Morphology of Cotton Seedlings Under Nitrogen Stress [J]. Scientia Agricultura Sinica, 2014, 47(23): 4595-4605.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd