Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (6): 1171-1179.doi: 10.3864/j.issn.0578-1752.2014.06.013

• HORTICULTURE • Previous Articles     Next Articles

Heat Stress Mitigation by Exogenous Nitric Oxide Application Involves Polyamine Metabolism and PSII Physiological Strategies in Ginger Leaves

 LI  Xiu, GONG  Biao, WANG  Yun, XU  Kun   

  1. College of Horticulture Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region, Taian 271018, Shandong
  • Received:2013-09-29 Online:2014-03-15 Published:2013-12-25

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Abstract: 【Objective】 Ginger is a thermophilic vegetable crop with the characteristics of not tolerating high temperature, which is easily damaged by high temperature. To investigate the relationship between exogenous nitric oxide and polyamine metabolism, as well as the regulation effect on PSII, mitigation effect of heat stress on ginger by nitric oxide was studied. 【Method】‘Laiwu Big Ginger’ was sandy cultured in a climate chamber under 12h/12h photoperiod, 28℃/18℃ (normal) and 38℃/28℃ (heat stress) conditions. Ginger root was treated with 0.1 mmol•L-1 sodium nitroprussiate (an nitric oxide donor) and sodium ferricyanide (reactant of SNP after releasing NO). Relative water content, chlorophyll concentration, electrolyte leakage, chlorophyll fluorescence parameters and polyamine metabolism of ginger leaves were investigated on 0, 5, 10, 15 and 20 d after treatment. 【Result】Electrolyte leakage significantly increased with prolonging stressed time, while relative water content and chlorophyll concentration significantly decreased. Chlorophyll fluorescence parameters including Fv/Fm, ФPSII, Fv′/Fm′ qP and P decreased, and NPQ, β/α-1 and D increased. Main pattern of PSII was shown that its photochemical activity of PSII was decreased for the energy metabolism pathway shifting from photochemical to non-photochemical activity. Under heat stress, free and conjugated polyamines were significantly accumulated and then decreased in different treatment times. Insoluble bound polyamine and Put/PAs ratio kept an increasing trend with prolonging stressed time. Relative water content, chlorophyll concentration, electrolyte leakage of ginger leaves was recovered by exogenous application of nitric oxide, which regulated chlorophyll fluorescence parameters to normalization. Also nitric oxide application improved polyamine metabolism to reduce Put/PAs ratio. 【Conclusion】 Disordered polyamine metabolism, damaged ginger leaves and PSII were shown under 38℃/28℃ conditions. Significant heat stress mitigation by exogenous nitric oxide application was shown in this research involving polyamine metabolism and PSII physiological strategies to improve heat stress tolerance in ginger.

Key words: ginger , heat stress , nitric oxide , psII , polyamine metabolism

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