Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (2): 372-379.doi: 10.3864/j.issn.0578-1752.2017.02.015

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Relaxed Effect of Caulis Lonicerae Extraction on Contraction of Intestinal Smooth Muscle of Rabbit in Vitro and Its Mechanism

WANG Hui, ZHANG HaiJuan, LI ZhiDong   

  1. School of Pharmaceutical Sciences, Linyi University, Linyi 276000, Shandong
  • Received:2016-01-05 Online:2017-01-16 Published:2017-01-16

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Abstract: 【Objective】Excessive gastrointestinal smooth muscle contraction cause abdominal pain and diarrhea diseases in clinical. Recently, Ca2+ channel blocker nifedipine and anticholinergic atropine are widely employed in the treatment of gastrointestinal excessive contraction. However, nifedipine has a potent negative inotropic and dromotropic effects with dose increase after long-term administration, and atropine has severe side-effects which highly limited its clinical utilization. Therefore, it is important to develop natural medicines with high activity, low toxicity and no residue. In this research, the effects of Caulis lonicerae extraction on spontaneous smooth muscle contractions in isolated rabbit small intestine were investigated and the possible mechanism was explored.【Method】The in vitro intestine movement experiment, was conducted and the BL-420E biologic function analysis system was employed. The influences of C. lonicerae extraction on spontaneous contractions of the rabbit intestinal smooth muscle were measured. The effects of C. lonicerae extraction on spasmodic muscle contractions were measured by using acetylcholine (ACh), histamine (HA) and BaCl2. The mechanism of C. lonicerae extraction was studied by using inositol 1,4,5-trisphosphate (IP3) receptor antagonist (heparin, HP), ryanodine receptor antagonist (ruthenium red, RR) and a nitric oxide synthase (NOS) inhibitor (Nitro-L-arginine methyl ester, L-NAME).【Result】The results showed that the extraction of Caulis lonicerae extraction dose-dependently inhibited the spontaneous contraction of intestinal smooth muscle. Caulis lonicerae extraction, at the concentration of 7.5 g·L-1, showed highly inhibition effects on the frequency of spontaneous contractions (P< 0.05). Meanwhile, at the concentration of 5 g·L-1, significantly inhibition effects on the amplitude of spontaneous contractions (P < 0.05) were detected. ACh, HA and BaCl2 significantly induced the amplitude of smooth muscle contractions, and C. lonicerae extraction notably inhibited the muscle contractions induced by ACh, HA and BaCl2 (P < 0.05 or P < 0.01). IP3 receptor antagonist heparin could strengthen the relaxed effects of C. lonicerae extraction on intestinal smooth muscle, but ryanodine receptor antagonist ruthenium red had no effect on the relaxation of C. lonicerae extraction. L-NAME inhibited the relaxation of C. lonicerae extraction (P < 0.01).【Conclusion】The extraction of C. lonicerae inhibited the frequency and amplitude of intestinal smooth muscle contractions of rabbits in vitro. The mechanism may be related to the increase of NO concentration in intestinal smooth muscle, and the inhibiting intracellular Ca2+ released via IP3 of sarcoplasmic reticulum.

Key words: extraction of Caulis lonicerae, rabbit, intestinal smooth muscle, calcium, nitric oxide

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