Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (8): 1558-1567.doi: 10.3864/j.issn.0578-1752.2012.08.011

• HORTICULTURE • Previous Articles     Next Articles

Construction of Cucumber-Sour Cucumber Chromosome Introgression Lines and Location of Fruit Related QTLs

 MENG  Jia-Li, LOU  Qun-Feng, ZHOU  Xiao-Hui, SHI  Jian-Lei, CHEN  Jin-Feng   

  1. 南京农业大学园艺学院/作物遗传与种质创新国家重点实验室，南京 210095
  • Received:2011-09-07 Online:2012-04-15 Published:2012-01-19

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Abstract: 【Objective】 A set of cucumber-sour cucumber chromosome introgression lines were constructed by using cultivated cucumber (Cucumis sativus L., 2n=14) cultivar ‘Beijingjietou’ as recurrent parent and the wild ‘sour cucumber’ (C. hystrix Chakr., 2n = 24) as donor parent by the way of marker-assisted selection, and QTL controlling cucumber fruit shape was detected initially. 【Method】 Fristly, through interspecific-backcrossing-selfing approach, a lot of cucumber-sour cucumber chromosome introgression lines were obtained. Then polymorphism of the parents was detected by 298 pairs of SSR markers which uniformly distributed in the cucumber genome. And then the cucumber-sour cucumber chromosome introgression lines which contained the wild sour cucumber chromosome segments from the strains were screened by using the detected differences between parents SSR markers. QTL analysis of three main traits affecting cucumber fruit shape(fruit length, fruit diameter, fruit length/diameter ratio) was conducted using t-test compared with the recurrent parent. 【Result】 A set of 50 cucumber-sour cucumber chromosome introgression lines was constructed. There are 149 chromosome introgression segments containing 61 different introgression segments were identified in the lines. The total length of the different segments was 259.95 cM, and the coverage was 45.37% in the cucumber genome. The length of the introgression segments ranged from 1.65 cM to 15.4 cM with an average of 5.41 cM. They distributed in 7 chromosomes of cucumber. A total of 13 QTL affecting cucumber fruit shape were detected in CSIL population initially. 【Conclusion】 In this research, a set of cucumber-sour cucumber introgression lines were created and QTL affecting cucumber fruit shape was detected initially. It provided new resources for utilization of genes from C. hystrix and also the material basis for fine mapping of quantitative traits in cucumber.

Key words: wild sour cucumber, chromosome introgression lines, marker-assisted selection, quantitative trait locus

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