利用回交和标记辅助选择快速培育高油酸花生品种及其评价

doi:10.3864/j.issn.0578-1752.2018.09.003

Abstract

Abstract: 【Objective】 The objectives of this research were (i) to establish an effective backcrossing approach combined with marker-assisted selection (MAS) of peanut high oleate breeding, (ii) to transform the elite peanut cultivars with normal oleate (NO) into high oleate (HO) ones in three years.【Method】Four cross combinations were designed named as BO-01, BO-02, BO-03 and BO-04 by selecting the elite peanut cultivars ZH16, ZH21, QH551 and XH13 with AABB genotype as recurrent parents, while JH13 with aabb genotype was selected as the high oleate donor parent. Via 1 cross, 4 backcrosses and 1 self-crossing, BC₄F₂ progenies were obtained. According to the separation rule of backcross and high homology sequence of ahFAD2A and ahFAD2B, F₁ and backcross progenies（BC₁F₁-BC₄F₁）were genotyped by sequencing of PCR products, in which ahFAD2A and ahFAD2B were amplified in one PCR reaction with F0.7/R3 primer pairs. AaBb genotype progenies were selected and used as male parent in next generation backcross. Self-crossing progenies (BC₄F₂- BC₄F₃）were examined by KASP（kompetitive allele specific PCR）to obtain aabb genotype progenies. Main agronomic traits, quality characters and disease resistance of aabb genotype progenies were investigated. The genetic backgrounds of progenies with aabb genotype derived from ZH 16×JH13 were evaluated with SSR markers. 【Result】In summary, during 3 years, we obtained 10，5，6 and 8 high oleate progenies with aabb genotype in 4 cross combinations, respectively. By evaluating their agronomic traits, quality and bacterial wilt resistance, high oleate peanut cultivars were obtained, which were highly consistent with their corresponding recurrent female parent. ZJ019, ZJ109, ZJ160 and ZJ805 with oleic content as 82.54%, 79.85%, 79.22% and 78.94%, are regarded as high oleic cultivars of ZH16, ZH21, QH551 and XH13.【Conclusion】By using backcross, off-season multiplication for advancing generation and molecular marker selection, normal oleate peanut cultivars were successfully and quickly transited into high oleate ones within 3 years.

Key words: peanut, high oleic acid, backcross, marker-assisted selection (MAS); cultivar

ZHANG ZhaoHua, WANG ZhiHui, HUAI DongXin, TAN JiaZhuang, CHEN JianHong, YAN LiYing, WANG XiaoJun, WAN LiYun, CHEN Ao, KANG YanPing, JIANG HuiFang, LEI Yong, LIAO BoShou. Fast Development of High Oleate Peanut Cultivars by Using Marker-Assisted Backcrossing and Their Evaluation[J].Scientia Agricultura Sinica, 2018, 51(9): 1641-1652.

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