Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (7): 1432-1438.doi: 10.3864/j.issn.0578-1752.2012.07.023

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Resistance Selection Against Clofentezine in Tetranychus urticae (Koch) and Change of Its Detoxification Enzymes Activity

 GAO  Xin-Ju, ZHANG  Zhi-Gang, DUAN  Xin-Le, SHEN  Hui-Min   

  1. 甘肃农业大学草业学院/草业生态系统省部共建教育部重点实验室/中-美草地畜牧业可持续发展中心，兰州 730070
  • Received:2011-07-04 Online:2012-04-01 Published:2012-01-11

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Abstract: 【Objective】The objective of this study is to assess the development trend of resistance and the activity change of detoxification enzyme of Tetranychus urticae (Koch). 【Method】 The experiment was carried out in laboratory by using the methods of biological assay and biochemical analysis.【Result】After selected with clofentezine for 21 generations, the resistance strain (Clo-R21) increased to 122.26-fold. Clo-R21 exhibited 21.80 and 10.66-fold moderate level cross-resistance to pyr-clofentezine and liuyangmycin. However, the strain had low level cross-resistance to abamectin, fenpropathrin, beta-cyfluthrin, cypermethrin, azocyclotin, and celastrusangulatus with 6.85, 5.45, 5.02, 3.88, 3.48, 1.68-fold, respectively, and negative resistance to fenbutatin oxide, fenpyroximate, hydramethylnon, separately with 0.72, 0.65, 0.55-fold. Synergist experiments with different metabolic inhibitors revealed that triphenyl phosphate (TPP), diethyl maleate (DEM), piperonyl butoxide (PBO) and synergistic phosphorus (SV1), resulted in 1.39, 1.70, 6.78 and 1.64-fold synergist ratios, respectively. The major resistant mechanism to clofentezine was the increasing activities of carboxylesterases (CarE), acid phosphatase (ACP), alkaline phosphatase (ALP), glutathione-S-transferase (GSTs) and mixed function oxidase (MFO). Compared to the susceptible strain, the enzyme activities in Clo-R21 developed 2.25, 2.04, 1.91, 1.98 and 26.68 times.【Conclusion】 Activity increasing of MFO may play an important role in the resistance to clofentezine, meanwhile CarE, ACP, ALP, and GSTs may also confere resistance to the Clo-R21 strain.

Key words: Tetranychus urticae (Koch), clofentezine, resistance, cross resistance, synergist, detoxifying enzymes

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