Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (7): 1257-1264.doi: 10.3864/j.issn.0578-1752.2012.07.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Cloning and Analysis of LeWRKY2 Gene

 SUN  Qing-Peng, LI  Na, YU  Yong-Kun, ZHAO  Fu-Kuan, WAN  Shan-Xia, PAN  Jin-Bao   

  1. 1.北京农学院生物技术学院，北京 102206
    2.北京农学院植物科技学院，北京 102206
  • Received:2011-06-29 Online:2012-04-01 Published:2011-11-25

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Abstract: 【Objective】Cloning of WRKY transcription factor from tomato can provide a basis for molecular mechanism of pathogen reaction in tomato. 【Method】 Using RT-PCR and RACE technology, the LeWRKY2 full length cDNA was cloned (GenBank accession: EU755368.1), and its function was predicted by bioinformatics tools. The real-time PCR technology was used to analyze the expression levels of LeWRKY2 gene when treated with JA, Botrytis cinerea and cycloheximide. 【Result】 The LeWRKY2 full length cDNA, consisted of 1 007 bp, was isolated from tomato. The bioinformatics analysis showed that it included an ORF of 471 bp. LeWRKY2 protein contained a WRKY domain and a C2H2 zinc finger motif, and it might have the function of transcription, transcriptional regulation and signal transduction. The LeWRKY2 gene expression levels were proportional to the JA treatment time in the range of 0-60 min, while in the range of 60-150 min, the LeWRKY2 gene expression levels were inversely proportional to the JA treatment time when treated with 100 µmol•L-1 JA. LeWRKY2 gene expression was induced by Botrytis cinerea, and the LeWRKY2 gene expression reached maximum abundance at 4 h. The transcript of LeWRKY2 gene was not dependent on the protein biosynthesis. 【Conclusion】 LeWRKY2 gene is an immediate early gene involved in tomato defense response.

Key words: tomato, gene cloning, LeWRKY gene, jasmonic acid

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