Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (7): 1235-1245.doi: 10.3864/j.issn.0578-1752.2012.07.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Analysis of the Relationship Between Differential Expression of Rice Gene and Heterosis

 ZHANG  Xiao-Meng, XIAO  Ning, ZHANG  Hong-Xi, FENG  Yan-Xia, LIU  Zhi-Xiao, GAO  Yong, DAI  Zheng-Yuan, CHEN  Jian-Min   

  1. 1.扬州大学生物科学与技术学院 江苏扬州 225009
    2.江苏里下河地区农业科学研究所/国家水稻改良中心南京分中心，江苏扬州225007
  • Received:2011-10-08 Online:2012-04-01 Published:2011-11-16

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Abstract: 【Objective】The strong and weak heterosis groups in rice were analyzed for investigating the relationship between differential expression patterns of gene and heterosis. 【Method】The differential display-PCR (DD-PCR) technology was used to analyze gene differentially expressed patterns for the nine F1 of Yangdao 6, Minghui 63 and Teqing varieties as male parent in tillering leaves, booting panicles and heading flag leaf. 【Result】At the same growth stage, four kinds of gene differentially expressed patterns, UNP1 (genes specifically expressed in female parent), UNP2 (genes specifically expressed in male parent), UNF1(genes specifically expressed in F1), ABF1 (genes specifically silenced in F1), were presented obvious difference between strong heterosis group G1(Yangdao 6 as male parent) and weak heterosis group G2 (Minghui 63 as male parent). Correlation analysis showed that UNP2 of tillering leaves was significantly positive corelated to yield per plant, and the expressed genes from the paternal were benefit to heterosis. The differentially expressed gene patterns existed obvious difference among three growth stages in the same heterosis group, which indicates that gene expression is spatial and temporal characteristic. Total nineteen differentially expressed gene fragments between G1 and G2 groups were acquired and distributed along one to eleven chromosomes except the twelfth chromosome. The function analysis result showed that they were mainly involved in material synthesis and transport, energy, carbohydrate metabolism pathways and so on. 【Conclusion】 The gene differential expression patterns in strong and weak heterosis group exist significant difference at different growth stages. The phenotypic differences could be reflected by gene differential expression patterns.

Key words: rice, heterosis, expressed patterns

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