Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (6): 1226-1234.doi: 10.3864/j.issn.0578-1752.2012.06.022

• RESEARCH NOTES • Previous Articles    

Cloning and Expression Analysis of A Stress-related Ta14S Gene from Wheat

 REN  Jiang-Ping, LIU  Hai-Lun, WANG  Xin-Guo, NIU  Hong-Bin, LI  Yong-Chun, WANG  Xiang, CHEN  Xin, YIN  Jun   

  1. 河南农业大学/国家小麦工程技术研究中心，郑州 450002
  • Received:2011-11-07 Online:2012-03-15 Published:2011-12-02

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Abstract: 【Objective】In this study, the objective is to clone the stress resistance-related genes, and analyze the expression pattern of those genes which aim to investigate the underlying molecular mechanism of stress resistance and provide candidate genes for stress-resistance plant breeding.【Method】 Based on the analysis of up-regulated EST obtained by cDNA chip, a full-length cDNA sequence was cloned from wheat through rapid amplification of cDNA ends (RACE) method and proper bioinformatics softwares were applied for characterizing the cloned genes and the deduced proteins. The gene expression characters were analyzed by the real time PCR.【Result】The full-length cDNA sequence designated as Ta14S from wheat was 1 056 bp in length, contains a 792 bp open reading frame (ORF) encoding a 263 amino acid proteins, with 11 bp in the 5' UTR and 253 bp in the 3' UTR. Similarity alignment of plant 14-3-3 proteins by DNAMAN revealed four high conserved domains. These domains were a potential substrate for protein kinase C, annexin-like domain, domain to bind transcription factor and nuclear export signal peptide, respectively. This result suggests that the Ta14S belongs to the family of 14-3-3. Real time PCR analysis revealed that the expression level of Ta14S was the highest in root than in leave and stem. The expression of Ta14S was steadily up-regulated in any of the time points under PEG and low temperature stress and the relative expression level was significantly higher than those of control within 6 h of ABA and low temperature stress treatments. This result suggests that Ta14S may be involved in stress resistance-related response of ABA signaling pathways in wheat roots.【Conclusion】A full-length cDNA of Ta14S was cloned from wheat and the typical protein interact binding domains were found in the deduced proteins. The expression patterns of Ta14S gene under drought, high and low temperature and ABA treatments showed that Ta14S might play an important regulation role under stress in wheat. The results provided important information for further studies on molecular regulation mechanism underlying abiotic stress resistance in wheat.

Key words: wheat (Triticum aestivum), 14-3-3 protein, stress, expression

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