Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (5): 832-839.doi: 10.3864/j.issn.0578-1752.2012.05.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Analysis of a Chalcone Synthase (CHS) Gene from Purple Potato (Solanum tuberosum)

 HU  Chao-Yang, ZHOU  You-Feng, GONG  Yi-Fu, JIN  Si, WANG  He-Yu, ZHAO  Qun-Fen   

  1. 1.宁波大学海洋学院/教育部应用海洋生物重点实验室，浙江宁波 315211
  • Received:2011-08-31 Online:2012-03-01 Published:2011-10-31

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Abstract: 【Objective】 The aim of this study was to clone the full length cDNA of chalcone synthase from purple potato, to analyze its expression at different organs and the relationship between CHS expression and anthocyanin accumulation after treated with different concentrations of GA3 and sucrose. 【Method】 The full-length cDNA of CHS was isolated from Solanum tuberosum by RT-PCR and RACE. Semi-quantitative RT-PCR was used to analyze the expression levels of StCHS in different organs, and the expression levels induced by GA3 and sucrose were adopted by semi-quantitative RT-PCR. The anthocyanin content of purple potato was measured by spectrophotometer method. 【Result】 The cloned full-length CHS was 1 490 bp in length, containing a 1 170 bp open reading frame (ORF), which encodes 389 amino acids. The deduced amino acids contained Cys164, Phe215, His303, and Asn336 active sites, which constitute the catalytic center of CHS. CHS is expressed at different levels in different organs, which was found to be expressed in stems, leaf stalks and leaves but not in roots, tubers or rachises. The anthocyanin accumulation was slightly induced by GA3 as well as the expression of CHS. The anthocyanin accumulation was strongly induced by sucrose, but the CHS expression was not notably induced. 【Conclusion】CHS gene was cloned from purple potato and its expression is tissue-specific. StCHS is a rate-limited gene in the flavonoid synthesis pathway of purple potato.

Key words: purple potato (Solanum tuberosum), chalcone synthase gene (CHS), anthocyanin, GA3, sucrose, gene expression

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