Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (22): 4714-4720.doi: 10.3864/j.issn.0578-1752.2011.22.020

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Optimal Protein Levels Required and Their Effects on Larval Antioxidation of Apis mellifera ligustica Spinola

 LI  Cheng-Cheng, YANG  Wei-Ren, XU  Bao-Hua, FENG  Qian-Qian   

  1. 1.山东农业大学动物科技学院,山东泰安 271018
  • Received:2010-12-09 Online:2011-11-15 Published:2011-03-18

Abstract: 【Objective】 The objective of this study is to research the effect of dietary crude protein levels on growth and larval antioxidation of Apis mellifera ligustica Spinola. 【Method】 Thirty cages of honey bees obtained from a local apiary were used in the study. Honey bees were randomly allocated into 6 groups (with 5 replications each and 1 cage in each replication). One of the groups was fed pure rape pollen only (control, group A) and others were fed the diets with crude protein levels at 15% (group B), 20% (group C), 25% (group D), 30% (group E) and 35% (group F), respectively.【Result】During the trial period, there was no significant effect of dietary crude protein on the population of bee colony (P>0.05). The hatchability and percentage of pupation of group F were significantly higher than those of group B (P<0.05). The larval protein of group E was significantly higher than that of groups A, B and C (P<0.05). With the dietary protein level increasing, there was no significant effect on T-SOD activities of larva (P>0.05), while the content of MDA was decreased (P<0.05). 【Conclusion】These results indicated that the population of colony did not differ significantly with the crude protein content in this experiment, and 30%-35% of dietary crude protein level was optimal to maintain the highest growth performance and antioxidant activities.

Key words: honey bee, protein level, growth and development, antioxidant activity

[1]Crailsheim K. The protein balance of the honey bee worker. Apidologie, 1990, 21(5): 417-429.

[2]Ribeiro M F, Duchateau M J, Velthuis H H W. Comparison of the effects of two kinds of commercially available pollen on colony development and queen production in the bumble bee Bombus terrestris L (Hymenoptera, Apidae). Apidologie, 1996, 27(3): 133-144.

[3]Mattlila H R, Otis G W. The effects of pollen availability during larval developmenton the behaviour and physiology of spring-reared honey beeworkers. Apidologie, 2006, 37(5): 533-546.

[4]Tasei J N, Aupinel P. Nutritive value of 15 single pollens and pollen mixes tested on larvae produced by bumblebee workers (Bombus terrestris, Hymenoptera: Apidae). Apidologie, 2008, 39(4): 397-409.

[5]Moritz B, Crailsheim K. Physiology of protein digestion in the migut of the honeybee (Apis mellifera L.). Journal of Insect Physiology, 1987, 33(12): 923-931.

[6]Zahra A, Talal M. Impact of pollen supplements and vitamins on the development of hypopharyngeal glands and on brood area in honey bees. Journal of Apicultural Science, 2008, 52(2): 5-11.

[7]DeGrandi-Hoffman G, Chen Y, Huang E, Huang M H. The effect of diet on protein concentration, hypopharyngeal gland development and virus load in worker honey bees (Apis mellifera L.). Journal of Insect Physiology, 2010, 56(9): 1184-1191.

[8]Page Jr R E, Peng C Y S. Aging and development in social insects with emphasis on the honey bee, Apis mellifera L.. Experimental Gerontology, 2001, 36(4): 695-711.

[9]Herbert E W, Shimanuki Jr H, Caron D. Optimum protein levels required by honey bees (Hymenoptera, Apidae) to initiate and maintain brood. Apidologie, 1977, 8(2): 141-146.

[10]曾志将. 养蜂学. 北京: 中国农业出版社, 2003: 27.

Zeng Z J. Apiology. Beijing: China Agriculture Press, 2003: 27. (in Chinese)

[11]李成成, 胥保华, 冯倩倩, 杨维仁. 蜜蜂的营养需要及其研究方法. 中国蜂业, 2010, 61(2): 14-15.

Li C C, Xu B H, Feng Q Q, Yang W R. The nutrition and research methods of honeybee. Apiculture of China, 2010, 61(2): 14-15. (in Chinese)

[12]袁德雨. 夏末秋初需保持蜜蜂群势不衰. 蜜蜂杂志, 2007(7): 38.

Yuan D Y. Maintain the population of colony late summer or early autumn. Journal of Bee, 2007(7): 38. (in Chinese)

[13]Keith D R, Delaplane S. Autumn priorities. American Bee Journal, 1995, 135(9): 605-606.

[14]DeGrandi-Hoffman G, Wardell G, Ahumada-Secura F, Rinderer T, Danka R, Pettis J. Comparisons of pollen substitute diets for honeybees: consumption rates by colonies and effects on brood and adult populations. Journal of Apicultural Research and Bee World, 2008, 47(4): 265-270.

[15]吴  静, 李建科. 蜜蜂 (Apis mellifera L.) 幼虫级型分化差异蛋白质组分析. 中国农业科学, 2010, 43(1): 176-184.

Wu J, Li J K. Proteomic analysis of the honeybee (Apis mellifera L.) caste differentiation between worker and queens bees larvae. Scientia Agricultura Sinica, 2010, 43(1): 176-184. (in Chinese)

[16]Somerville D. Honey bee nutrition and supplementary feeding. NSW Agriculture, 2000, 6: 4-8.

[17]任付珍. 箱内存蜜与蜂群强壮的关系. 蜜蜂杂志, 2008, 9: 18.

Ren F Z. The relationship between stored honey and the population of honey. Journal of Bee, 2008, 9: 18. (in Chinese)

[18]Piffanelli P, Ross J H E, Murphy D J. Biogenesis and function of the lipidic structures of pollen grains. Sexual Plant Reproduction, 1998, 11: 65-80.

[19]Wei H, Cai Q, Rahn R O. Inhibition of UV light and Fenton reaction-induced oxidation DNA damage by the soybean isoflavone genistein. Carcinogenesis, 1996, 17(1): 73-77.

[20]Kondoh M, Kamada K, Kuronaga M, Higashimoto M, Takiguchi M, Watanabe Y, Sato M. Antioxidant property of metallothionein in fasted mice. Toxicology Letters, 2003, 143: 301-306.

[21]Alul R H, Wood M, Longo J, Marcotte A L, Campione A L, Moore M K, Lynch S M. Vitamin C protects low-density lipoprotein from homocysteine-mediated oxidation. Free Radical Biology and Medicine, 2003, 34(7): 881-891.

[22]谷春梅, 施用辉, 乐国伟. 过量食入蛋白对小鼠胰腺的氧化损伤. 安徽农业科学, 2010, 38(25): 13730-13731.

Gu C M, Shi L H, Le G W. The oxidative damage of excessive protein ingestion on pancreas of mice. Journal of Anhui Agricultural Science, 2010, 38(25): 13730-13731. (in Chinese)

[23]翁锡全, 林洁如, 林文韬, 黄丽英,王仁纲, 吴桂贤. 低氧暴露对运动性贫血大鼠抗氧化能力的影响. 中国组织工程与临床康复, 2007, 11(17): 3357-3360.

Weng X Q, Lin J R, Lin W T, Huang L Y, Wang R G, Wu G X. Influence of intermittent hypoxic exposure on the antioxidation in rats of sports anemia. Journal of Clinical Rehabilitative Tissue Engineering Research, 2007, 11(17): 3357-3360. (in Chinese) 

[24]Hunter E A L, Grimble R F. Cysteine and methionine supplementation modulate the effect of tumor necrosis factor α on protein synthesis, glutathione and zinc concentration of liver and lung in rats fed a low protein diet. The Journal of Nutrition, 1994, 124(12): 2319-2328.

[25]张佃志, 方允中. 十四种化合物的抗氧化活性. 生物物理学报, 1990, 6(3): 383-387.

Zhang T Z, Fang Y Z. Study on the antioxidative activity of fourteen kinds of compounds. Acta Biophysica Sinica, 1990, 6(3): 383-387. ( in Chinese)
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