华南地区副猪嗜血杆菌的耐药性特点及四环素耐药基因携带情况

doi:10.3864/j.issn.0578-1752.2011.22.021

Abstract

Abstract: 【Objective】 Haemophilus parasuis is one of the most frequent pathogenic bacteria which has caused a serious of problems in pig industry all over the world. Therefore, it is important to evaluate the resistance to antibiotics and the distribution of tetracycline resistance determinants in H. parasuis from pigs in order to prevent and control this diseases. 【Method】 All H. parasuis were isolated from clinically diseased pigs and antimicrobial susceptibility to 21 antibiotics was performed using the broth microdilution method, and PCR was used to detect the presence of 9 tetracycline resistance genes. Then genetic relationship of the isolates which had tetracycline resistance was analyzed by Pulsed-?eld gel electrophoresis (PFGE). 【Result】 Most of the H. parasuis isolates showed resistance to many antimicrobial agents commonly used in veterinary clinic such as sulfamethoxazole, ?uoroquinolones, tetracycline, and gentamicin. Especially, more than 80% of the isolates were resistant to trimethoprim and sulfamethoxazole. Cephalosporins showed a good activity to H. parasuis isolates. Overall, the antimicrobial resistance of H. parasuis isolated from South China between 2008 and 2010 has been rising. All isolates showed multiple-drug resistance, resistance to 7 and 8 antibiotics was the main trend, the percentage were 19.13% and 17.39%. Tetracycline resistance gene tetB was widespread in H. parasuis. Analysis of PFGE typing implied that the isolates represented a wide variety of chromosomal backgrounds. 【Conclusion】 The study showed high resistance to antibiotics in H. parasuis isolated from pigs in South China, and tetB gene was widespread. The efflux of the drugs may be the major resistance mechanism of H. parasuis to tetracycline. This study helps to grasp the trend of drug resistance, and reveals mechanism of bacteria resistance to tetracycline.

Key words: Haemophilus parasuis, antimicrobial susceptibility, tetracycline resistance gene

XU Cheng-Gang, GUO Li-Li, ZHANG Jian-Min, ZHANG Bin, LI Ang, CHEN Ji-Cheng, LI Jing-Yi, LIAO Ming. Resistance to Antibiotics and Distribution of Tetracycline Resistance Determinants in Haemophilus parasuis from Pigs in South China[J].Scientia Agricultura Sinica, 2011, 44(22): 4721-4727.

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Resistance to Antibiotics and Distribution of Tetracycline Resistance Determinants in Haemophilus parasuis from Pigs in South China

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