Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (22): 4700-4705.doi: 10.3864/j.issn.0578-1752.2011.22.018

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Molecular Cloning, Sequence Analysis and Tissue Expression of Follistatin Gene in Dazu Black Goat

 LUO  Yan-Mei, ZHANG  Chao, ZHAO  Zhong-Quan, FAN  Jing-Sheng, ZHANG  Jia-Hua   

  1. 1.西南大学重庆市牧草与草食家畜重点实验室，重庆400716
    2.四川省畜牧科学研究院，成都610066
  • Received:2010-11-17 Online:2011-11-15 Published:2011-03-09

PDF

443

Cited

6

Abstract: 【Objective】 The objective of this experiment is to clone the sequence of Dazu black goat follistatin gene and explore the expression levels of follistatin gene in different tissues.【Method】The cDNA of follistatin gene was cloned from total RNA of ovary tissue in Dazu Black Goat by RT-PCR. The expression analysis of follistatin gene was predicted with some related software tools,and the expression of follistin gene in different tissues was analyzed with real-time PCR.【Result】Sequence analysis indicated that the cloned follistatin gene cDNA was 1 217 bp in length (HM138665) containing an entire open reading frame (ORF), and encoding 344 amino acids. According to the Blast result in GenBank database, the cDNA nucleotides share 98% homology with Bos taurus. Real-time PCR analysis showed that the follistatin gene was expressed in spleen, lung, kidney, liver, heart, ovary and pituitary. The expression levels of follistatin gene were higher in kidney and lower in pituitary. The expression level of follistatin gene in kidney and heart were significantly higher than in pituitary (P＜0.05). No significant difference was found between the other tissues (P＞0.05).【Conclusion】The sequence of follistatin gene cDNA was obtained. And the follistatin mRNA expression was the hingest in kidney.

Key words: follistatin, real-time fluorescence quantitative, RT-PCR, tissue expression, Dazu Black Goat

[1]赵中权, 张家骅, 李周权, 王贤海, 陈长庚, 徐恢仲. 大足黑山羊种质特性. 中国畜牧杂志, 2008, 44(15): 9-10.

Zhao Z Q, Zhang J H, Li Z Q, Wang X H, Chen C G, Xu H Z. Germplasm characteristics of Dazu Black Goat. Chinese Journal of Animal Science, 2008, 44(15): 9-10. (in Chinese)

[2]Silva C C, Knight P G. Modulatory action of activin A and follistatin on the developmental competence of in vitro matured bovine oocytes. Biology of Reproduction, 1998, 58: 558-565.

[3]Robertson D M, Klein R, de Vos F L, McLachlan R I, Wettenhall R E, Hearn M T, Burger H G, de Kretser D M. The isolation of polypeptides with FSH suppressing activity from bovine follicular fluid which are structurally differernt to inhibin. Biochemical and Biophysical Research Communications, 1987, 149: 744-749.

[4]Ueno N, Ling N, Ying S Y, Esch F, Shimasaki S, Guillemin R. Isolation and partial characterization of FS: a single chain Mr 35,000 monomeric protein that inhibits the release of follicle-stimulating hormone. Proceedings of the National Academy of Sciences of the United States of America, 1987, 84: 8282-8286.

[5]Phillips D J, de Kretser D M. Follistatin: a multifunctional regulatory protein. Front Neuroendocrinol, 1998, 19(4): 287-322.

[6]Sadatsuki M, Tsutsumi O, Yamada R, Muramatsu M, Taketani Y. Local regulatory effects of activin A and follistatin on meiotic maturation of rat oocytes. Biochemical and Biophysical Research Communitions, 1993, 196: 388-395.

[7]Arai KY, Ohshima K, Watanabe G, Arai K, Uehara K, Taya K. Dynamics of messenger RNAs encoding inhibin/activin subunits and follistatin in the ovary during the rat estrous cycle. Biology Reporoduction, 2002, 66(4): 1119-1126.

[8]Shukovski L, Zhang Z W, Michel U, Findlay J K. Expression of mRNA for follicle stimulating hormone suppressing protein in ovarian tissues of cows. Journal of Reprodcution Fertility, 1992, 95: 861-867.

[9]Findnay J K. An update on the role of inhibin activin and follistatin as local regulator of folliculogenesis. Biology of Reproduction, 1993, 48: 15-23.

[10]Shimasaki S, Koga M, Buscaglia M L, Simmons D M, Bicsak T A, Ling N. Follistatin gene expression in the ovary and extragonadal tissues. Molecular Endocrinology, 1989, 4, 3(4): 651-659.

[11]Jaswant S, Greff P A. Immunohistochemical distribution of follistatin in dominant and subordinate folliceles and corpus luteum of cattle. Biology of Reproduction, 1998, 59: 561-570.

[12]Gamer L W, Wolfman N M, Celeste A J. A novel BMP expressed in developing mouse limb, spinal cord, and tail bud is a potent mesoderm inducer in Xenopus embryos. Developmental Biology, 1999, 208: 222-232.

[13]Hashimoto M, Nakamura T, lnoue S. Follistatin is a developmentally regulated cytokine in neural differentiation. The Journal of Biological Chemistry, 1992, 267: 7203-7206.

[14]Hashimoto M, Shoda A, Inous S,Yamada R, Kondo T, Sakurai T, Ueno N, Maramatsu M. Functional regulation of osteoblastic cells by the interaction of activin-A with follistatin. The Journal of Biological Chemistry, 1991, 267: 4999-5004.

[15]Martin M, Matzuk , Nalfang LU, Hannes Vogel, Klaus sellheyer, Denns R, Multiple defects andperinatal death in mice deficient in follistatin. Nature, 1995, 374: 360-363.

[16]Shiozaki M, Sakai R, Tabuchi M. Evidence for the participation of endogenous activin-A/erythroid differentiation factor in the regulation of erythropoiesis. Proceedings of the National Academy of Sciences of the United States of America, 1992, 82: 1553-1556.

[17]Depaolo L V, Bicsak T A, Eriskson G F, Shimasaki S, Ling N. Follistatin and activin: a potential intrinsic regulatory system within diverse tissue. Proceedings of the Society for Experimental Biology and Medicine, 1991, 198: 500-512.

[18]Welt C, Sidis Y, Keutmann H, Schneyer A. Activins,inhibins,and follistatins: from endocrinology to signaling.A paradigm for the new millennium. Experimental Biology and Medicine (May wood), 2002, 227(9): 724-752.

[19]王杏龙, 丁志丽. 波尔山羊发情后排卵时间的研究. 中国畜牧杂志, 2004, 40(9): 56-57.

Wang X L, Ding Z Y. Rearch on the boer goat about time of ovulation after estrus. Chinese Journal of Animal Science, 2004, 40(9): 56-57. (in Chinese)

[20]杨孟伯, 杨  莉, 王鲜忠, 张家骅. 大足黑山羊卵泡发育与内分泌激素分析. 中国草食动物, 2009, 29(2): 8-11.

Yang M B, Yang L, Wang X Z, Zhang J H. Research on endocrine hormone and follicular development in Dazu Black Goat. China Herbivores, 2009, 29(2): 8-11. (in Chinese)

[21]David J P, David M K, Follistatin: a multifunctional regulatory protein. Frontiers in Neuroendocrinolofy, 1998, 19(4): 287-332.

[22]张巧灵, 姜俊兵, 贺俊平, 赫晓燕, 耿建军, 董常生. 催乳素受体基因与羊驼繁殖性能关系的初探. 激光生物学报, 2007, 16(1): 18-21.

Zhang Q L, Jiang J B, He J P, He X Y, Gen J J, Dong C S. A study on the relationship between prolactin receptor gene and reproductive capability of Alpaca (Lama pacos). Acta Laser Biology Sinica, 2007, 16(1): 18-21. (in Chinese)
[1] WANG YiDan,YANG FaLong,CHEN DiShi,XIANG Hua,REN YuPeng. One-Step Multiple TaqMan Real-time RT-PCR for Simultaneous Detection of Swine Diarrhea Viruses [J]. Scientia Agricultura Sinica, 2023, 56(1): 179-192.
[2] WANG SiTong,CHEN Yan,LUO YuJia,YANG YuanYuan,JIANG ZhiYang,JIANG XinYi,ZHONG Fan,CHEN Hao,XU HongXing,WU Yan,DUAN HongXia,TANG Bin. Effect of Three Novel Compounds on Trehalose and Chitin Metabolism and Development of Spodoptera frugiperda [J]. Scientia Agricultura Sinica, 2022, 55(8): 1568-1578.
[3] XIE LiXue,ZHANG XiaoYan,ZHANG LiJie,ZHENG Shan,LI Tao. Complete Genome Sequence Characteristics and TC-RT-PCR Detection of East Asian Passiflora Virus Infecting Passiflora edulis [J]. Scientia Agricultura Sinica, 2022, 55(22): 4408-4418.
[4] KANG JunMei,ZHANG QiaoYan,JIANG Xu,WANG Zhen,ZHANG TieJun,LONG RuiCai,CUI HuiTing,YANG QingChuan. Cloning MsSQE1 from Alfalfa and Functional Analysis in Saponin Synthesis [J]. Scientia Agricultura Sinica, 2020, 53(2): 247-260.
[5] XU JianJian,WANG YanJiao,DUAN Yu,MA ZhiMin,BIN Yu,ZHOU ChangYong,SONG Zhen. Construction of Genome-Length cDNA of Citrus Vein Enation Virus and Identification of Its Infectivity [J]. Scientia Agricultura Sinica, 2020, 53(18): 3707-3715.
[6] ZHANG DaoWei,KANG Kui,YU YaYa,KUANG FuPing,PAN BiYing,CHEN Jing,TANG Bin. Characteristics and Immune Response of Prophenoloxidase Genes in Sogatella furcifera [J]. Scientia Agricultura Sinica, 2020, 53(15): 3108-3119.
[7] LIU YiRan,ZHANG Hong,JIN JiSu,ZHOU ZhongShi,GUO JianYing. Identification and Expression Analysis of the Halloween Gene Family in Agasicles hygrophila [J]. Scientia Agricultura Sinica, 2020, 53(10): 2009-2019.
[8] DING YanJuan,LIU YongKang,LUO YuJia,DENG YingMei,XU HongXing,TANG Bin,XU CaiDi. Potential Functions of Nilaparvata lugens GSK-3 in Regulating Glycogen and Trehalose Metabolism [J]. Scientia Agricultura Sinica, 2019, 52(7): 1237-1246.
[9] TIAN ZhiLong,TANG JiShun,SUN Qing,WANG YuQin,ZHANG XiaoSheng,ZHANG JinLong,CHU MingXing. Tissue Expression and Polymorphism of Sheep SMAD1 Gene and Their Association with Litter Size [J]. Scientia Agricultura Sinica, 2019, 52(4): 755-766.
[10] TANG Bin,SHEN QiDa,ZENG BoPing,XIAO ZhongJiu,QIU LingYu,PAN BiYing,LI Kun,ZHANG DaoWei. Characteristics, Developmental Expression and RNAi Effect Analysis of a Novel Trehalose-6-Phosphate Synthase Gene in Nilaparvata lugens [J]. Scientia Agricultura Sinica, 2019, 52(3): 466-477.
[11] JunBo PENG,XingHong LI,Wei ZHANG,Ying ZHOU,JinBao HUANG,JiYe YAN. Pathogenicity and Gene Expression Pattern of the Exocrine Protein LtGH61A of Grape Canker Fungus [J]. Scientia Agricultura Sinica, 2019, 52(24): 4518-4526.
[12] WAN DongLi,HOU XiangYang,DING Yong,REN WeiBo,WANG Kai,LI XiLiang,WAN YongQing. Response and the Expression of Pi-Responsive Genes in Leymus chinensis Under Inorganic Phosphate Treatment [J]. Scientia Agricultura Sinica, 2019, 52(23): 4215-4227.
[13] YUAN JunHu,DING YiJuan,YANG WenJing,YAN BaoQin,CHAI YaRu,MEI JiaQin,QIAN Wei. Identification of Genes Encoding Secretory Proteins Related to the Pathogenicity of Sclerotinia sclerotiorum Using TRV-HIGS [J]. Scientia Agricultura Sinica, 2019, 52(23): 4274-4284.
[14] LIU FanQi,WAN GuiJun,ZENG LuYing,LI ChunXu,PAN WeiDong,CHEN FaJun. Selection of Stable Internal Reference Genes for Transcript Expression Analyses in Laodelphax striatellus Under Near-Zero Magnetic Field [J]. Scientia Agricultura Sinica, 2019, 52(19): 3346-3356.
[15] ZHANG DaoWei,YU YaYa,PAN BiYing,KANG Kui,ZENG BoPing,CHEN Jing,TANG Bin. Regulation Function of Trehalose-6-phosphate Synthase Genes on Chitin Synthesis in Sogatella furcifera [J]. Scientia Agricultura Sinica, 2019, 52(19): 3357-3366.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd