Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (22): 4694-4699.doi: 10.3864/j.issn.0578-1752.2011.22.017

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Detection of Quantitative Trait Loci for Live Weight at Weaning in an Erhualian×Shaziling Population Using Whole-Genome High-Density SNP Markers

 HE  Yu-Yong, LI  Wan-Bo, ZHANG  Zhi-Yan, YANG  Ming, 欧Yang-Jing , YANG  Jie, REN  Jun, XIAO  Shi-Jun   

  1. 1.江西农业大学动物生物技术国家重点实验室培育基地，南昌 330045
  • Received:2010-01-30 Online:2011-11-15 Published:2011-04-15

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Abstract: 【Objective】An Erhualian×Shaziling F2 resource population was constructed to map quantitative trait loci (QTL) for liveweight of piglets at the weaning age of 45 days and identify positional candidate genes related to weaned liveweight, providing a start pointing to detect the causal genes in the future.【Method】The F2 resource population was constructed and genomic DNA of all weaned piglets was genotyped with Illumina porcine 60 k DNA chip. Whole-genome linkage analysis was conducted to detect QTL for liveweight of piglets weaned at day 45. Positional candidate genes were characterized from Ensemble (EMBL-EBI) and NCBI (National Center for Biotechnology Information) database.【Result】A total of 3 QTLs were detected for the measured phenotype including one 5% genome-wide QTL on sus scrofa chromosome 2 (SSC2) and one 1% genome-wide QTL on sus scrofa chromosome 5 (SSC5) and sus scrofa chromosome 14 (SSC14), respectively. Five candidate genes were identified, including CYP2R1, COPB1 and PDE3B on SSC2 and NOP2, GDF3 on SSC5. 【Conclusion】 The QTL for liveweight of piglets weaned at day 45 were mapped on SSC2, SSC5 and SSC14, and 5 positional candidate genes were identified.

Key words: swine, liveweight at weaning, QTL mapping, candidate gene, whole-genome linkage analysis

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