Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (22): 4700-4705.doi: 10.3864/j.issn.0578-1752.2011.22.018

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Molecular Cloning, Sequence Analysis and Tissue Expression of Follistatin Gene in Dazu Black Goat

 LUO  Yan-Mei, ZHANG  Chao, ZHAO  Zhong-Quan, FAN  Jing-Sheng, ZHANG  Jia-Hua   

  1. 1.西南大学重庆市牧草与草食家畜重点实验室，重庆400716
    2.四川省畜牧科学研究院，成都610066
  • Received:2010-11-17 Online:2011-11-15 Published:2011-03-09

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Abstract: 【Objective】 The objective of this experiment is to clone the sequence of Dazu black goat follistatin gene and explore the expression levels of follistatin gene in different tissues.【Method】The cDNA of follistatin gene was cloned from total RNA of ovary tissue in Dazu Black Goat by RT-PCR. The expression analysis of follistatin gene was predicted with some related software tools,and the expression of follistin gene in different tissues was analyzed with real-time PCR.【Result】Sequence analysis indicated that the cloned follistatin gene cDNA was 1 217 bp in length (HM138665) containing an entire open reading frame (ORF), and encoding 344 amino acids. According to the Blast result in GenBank database, the cDNA nucleotides share 98% homology with Bos taurus. Real-time PCR analysis showed that the follistatin gene was expressed in spleen, lung, kidney, liver, heart, ovary and pituitary. The expression levels of follistatin gene were higher in kidney and lower in pituitary. The expression level of follistatin gene in kidney and heart were significantly higher than in pituitary (P＜0.05). No significant difference was found between the other tissues (P＞0.05).【Conclusion】The sequence of follistatin gene cDNA was obtained. And the follistatin mRNA expression was the hingest in kidney.

Key words: follistatin, real-time fluorescence quantitative, RT-PCR, tissue expression, Dazu Black Goat

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