Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (15): 3235-3245.doi: 10.3864/j.issn.0578-1752.2011.15.019

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Localization and Differential Expression of LHR and LHR mRNA in the Oviduct of Jining Gray Goat During Estrous Cycle

LI  Guang-Jun, WANG  Hui, WANG  Shu-Ying, HOU  Yan-Meng, HUANG  Li-Bo   

  1. 1.山东农业大学动物科技学院
    2.山东农业大学动物科技学院/动物生物技术与疫病防治重点实验室
  • Received:2010-07-22 Revised:2010-11-05 Online:2011-08-01 Published:2010-11-05

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Abstract: 【Objective】 The objective of the experiment is to study the localization of LH receptor (LHR) and the expression of LHR mRNA in the oviduct during estrous cycle in Jining Gray Goat. 【Method】 The target fragment of LHR mRNA was retrieved, cloned and analyzed by using the real time fluorescence quantitative PCR, then the differences in expression were detected. At the same time, the immunohistochemical staining of LHR was done. 【Result】 The localizations of LHR immunoreactive substance in all parts of the oviduct at different stages of estrous cycle were carried out respectively, and it was found that LHR is mainly distributed on the epithelium of tubal mucosa, lamina propria and blood vessel endothelium. LHR mRNA expression was different in the oviduct of Jining Gray Goat at the four estrous cycle phrases. LHR mRNA in the linfundibulum was the highest during estrus (P＜0.01). In the ampolla, was the lowest during metestrus (P＜0.05). In the isthmus, was the highest during proestrus (P＜0.01). 【Conclusion】 The localization of LHR and its mRNA expression in the oviduct of goat reveals that LH has a kind of administration on the function of ovidct.

Key words: Gray Goat, LHR, RT-PCR, immunohistochemistry, oviduct

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