Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (15): 3235-3245.doi: 10.3864/j.issn.0578-1752.2011.15.019

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Localization and Differential Expression of LHR and LHR mRNA in the Oviduct of Jining Gray Goat During Estrous Cycle

LI  Guang-Jun, WANG  Hui, WANG  Shu-Ying, HOU  Yan-Meng, HUANG  Li-Bo   

  1. 1.山东农业大学动物科技学院
    2.山东农业大学动物科技学院/动物生物技术与疫病防治重点实验室
  • Received:2010-07-22 Revised:2010-11-05 Online:2011-08-01 Published:2010-11-05

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Abstract: 【Objective】 The objective of the experiment is to study the localization of LH receptor (LHR) and the expression of LHR mRNA in the oviduct during estrous cycle in Jining Gray Goat. 【Method】 The target fragment of LHR mRNA was retrieved, cloned and analyzed by using the real time fluorescence quantitative PCR, then the differences in expression were detected. At the same time, the immunohistochemical staining of LHR was done. 【Result】 The localizations of LHR immunoreactive substance in all parts of the oviduct at different stages of estrous cycle were carried out respectively, and it was found that LHR is mainly distributed on the epithelium of tubal mucosa, lamina propria and blood vessel endothelium. LHR mRNA expression was different in the oviduct of Jining Gray Goat at the four estrous cycle phrases. LHR mRNA in the linfundibulum was the highest during estrus (P＜0.01). In the ampolla, was the lowest during metestrus (P＜0.05). In the isthmus, was the highest during proestrus (P＜0.01). 【Conclusion】 The localization of LHR and its mRNA expression in the oviduct of goat reveals that LH has a kind of administration on the function of ovidct.

Key words: Gray Goat, LHR, RT-PCR, immunohistochemistry, oviduct

[1] 王福刚, 何绍钦, 刘召乾. 论青猾皮国际市场需求对济宁青山羊养殖业的影响. 中国畜禽种业, 2008, 11: 10-13.Wang F G, He S Q, Liu Z Q. The impact of the international marketdemand for leather of Jining Grey Goats to its aquaculture. TheChinese Livestock and Poultry Breeding, 2008, 11: 10-13. (inChinese)

[2] Zheng M L, Shi H, Segaloff D L, van Voorhis B J. Expression andlocalization of luteinizing hormone receptor in the female mousereproductive tract. Biology of Reproduction, 2001, 64: 179-187.

[3] Lei Z M, Toth P, Rao C V, Pridham D. Novel coexpression of humanchorionic gonadotropin (hCG)/human luteinizing hormone receptorsand their ligand hCG in human Fallopian tubes. Clinical EndocrinolgyMetabilism, 1993, 77: 863-872.

[4] Han S W, Lei Z M, Rao C V. Up-regulation of cyclooxygenase-2 geneexpression by chorionic gonadotropin in mucosal cells from humanfallopian tubes. Endocrinology, 1996, 137: 2929-2937.

[5] Sun T, Lei Z M, Rao C V. A novel regulation of the oviductalglycoprotein gene expression by luteinizing hormone in bovine tubalepithelial cells. Molecular and Cellular Endocrinology, 1997, 131:97-108.

[6] Mishra S, Lei Z M, Rao C V. A novel role of luteinizing hormone inthe embryo development in cocultures. Biology of Reproduction, 2003,68: 1455-1462.

[7] Shemesh M, Mizrachi D, Gurevich M, Shore L S, Reed J, Chang S M,Thatcher W W, Fields M J. Expression of functional luteinizinghormone (LH) receptor and its messenger ribonucleic acid in bovineendometrium: LH augmentation of cAMP and inositol phosphate invitro and human chorionic gonadotropin (hCG) augmentation ofperipheral prostaglandin in vivo. Biology of Reproduction, 2001, 1(2):13-32.

[8] Shemesh M, Mizrachi D, Gurevich M, Stram Y, Shore L S, Fields M J.Functional importance of bovine myometrial and vascular LHreceptors and cervical FSH receptors. Seminars in ReproductiveMedicine, 2001, 19(1): 87-96.

[9] Derecka K, Pietilä E M, Rajaniemi H J, Zi?cik A J. Cycle dependentLH/hCG receptor gene expression in porcine nongonadal reproductivetissues. Journal of Physiology and Pharmacology, 1995, 46: 77-85.

[10] Gawronska B, Paukku T, Huhtaniemi I, Wasowicz G, Ziecik A J.Oestrogen-dependent expression of LH/ hCG receptors in pigfallopian tube and their role in relaxation of the oviduct. Reproductionand Fertility, 1999, 115: 293-301.

[11] Gawronska B, Stepien A, Ziecik A J. Effect of estradiol andprogesterone on oviductal LH-receptors and LH-dependent relaxationof the porcine oviduct. Theriogenology, 2000, 53: 659-672.

[12] Rzucidlo S J, Weigl R M, Tilton J E. Myometrial LH/hCG receptorsduring the estrous cycle and pregnancy in pigs. Animal ReproductionScience, 1998, 51: 249-257.

[13] Han S W, Lei Z M, Rao C V. Up-regulation of cyclooxygenase-2 geneexpression by chorionic gonadotropin during the differentiation ofhuman endometrial stromal cells into deciduas. Endocrinology, 1996,137(5): 1791-1797.

[14] Donnez J, Casanas-Roux F, Caprasse J, Ferin J, Thomas K. Cyclicchanges in ciliation, cell height, and mitotic activity in human tubalepithelium during reproductive life. Fertility and Sterility, 1985, 43(4):554-559.

[15] Leese H J, Tay J I, Reischl J, Downing S J. Formation of fallopiantubal fluid: role of a neglected epithelium. Reproduction, 2001, 121(3):339-346.

[16] Rief S, Sinowatz F, Stojkovic M, Einspanier R, Wolf E, Prelle K.Effects of a novel co-culture system on development, metabolism andgene expression of bovine embryos produced in vitro. Reproduction,2002, 124(4): 543-556.

[17] Hunter R H F. Pre-ovulatory arrest and peri-ovulatory redistribution ofcompetent spermatozoa in the isthmus of the pig oviduct. Journal ofReproduction and Fertility, 1984, 72(1): 203-211.

[18] Schaefer M, Hofmann T, Schultz G, Gudermann T. A newprostaglandin E receptor mediates calcium influx and acrosomereaction in human spermatozoa. Proceedings of the National Academyof Sciences of the United States of America, 1998, 95(6): 3008-3013.

[19] Segi E, Haraguchi K, Sugimoto Y, Tsuji M, Tsunekawa H, Tamba S,Tsuboi K, Tanaka S, Ichikawa A. Expression of messenger RNA forprostaglandin E receptor subtypes EP4/EP2 and cyclooxygenaseisozymes in mouse periovulatory follicles and oviducts duringsuperovulation. Biology of Reproduction, 2003, 68(3): 804-811.

[20] You S, Kim H, Hsu C C, EL Halawani M E, Foster D N. Threedifferent turkey luteinizing hormone receptor (tLH-R) isoforms I:characterization of alternative spliced tLH-R isoforms and theirregulated expression in diverse tissues. Biology of Reproduction, 2000,62: 108-116.

[21] Weems Y S, Kim L, Humpherys V, Tsuda V, Weems C W. Effect ofluteinizing hormone (LH), pregnancy specific protein B (PSPB), orarachidonic acid (AA) on ovine endometrium of the estrous cycle orplacental secretion of prostaglandins E2 (PGE2) and F2α(PGF2α) andprogesterone in vitro. Prostaglandins, 2003, 71: 55-73.

[22] Sawitzke A L, Odell W D. Uterine binding sites for LH/hCG can bemodulated by hormonal status in rabbits and rats. ActaEndocrinologica, 1991, 124: 322-330.

[23] Fields M J, Shemesh M. Extragonadal luteinizing hormone receptorsin the reproductive tract of domestic animals. Biology of Reproduction,2004, 71: 1412-1418.

[24] Allen E. The oestrous cycle in the mouse. American Journal ofAnatomy, 2005, 30: 297-371.

[25] Espinasse P G. The oviductal epithelium of the mouse. AmericanJournal of Anatomy, 1935, 69: 363-368.

[26] 于 一, 张作千. 动情周期中小白鼠生殖系统的组织化学观察Ⅲ.输卵管. 解剖学报, 1959, 4(1-2): 39-50.Yu Y, Zhang Z Q. Histochemical observation on the femalereproductive organs in the mouse at different stages of the estrousctcle Ⅲ.oviduct. Acta Anatomica Sinica, 1959, 4(1): 39-50. (inChinese)

[27] 金光明, 杨 倩, 王 珏, 李升和, 刘胜兵, 李玉磊. 猪雌性生殖道黏膜显微和超微结构观察. 畜牧兽医学报, 2005, 36(6): 612-615.Jin G M, Yang Q, Wang J, Li S H, Li S B, Li Y L. Observation on themicrostructure and ultrastructure of genital tract mucosa of sow. ActaVeterinaria et Zootechnica Sinica, 2005, 36(6): 612-615. (in Chinese)

[28] Murray M K. Epithelial lining of the sheep ampulla oviduct undergoespregnancy associated morphological changes in secretory status andcell height. Biology of Reproduciton, 1995, 53(3): 653-663.

[29] 谭 娟, 余四九, 崔 燕. 妊娠期牦牛输卵管的组织结构观察. 中国兽医科学, 2008, 38(7): 622-625.Tan J, Yu S J, Cui Y. Observation of the histological structure of bosgrunniens oviduct during pregnancy. Chinese Veterinary Science, 2008,38(7): 622-625. (in Chinese)
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