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QTL Analysis for Tubular Floret Number and Flower Center Diameter of Chrysanthemum

ZHANG Fei; CHEN Fa-di; FANG Wei-min; CHEN Su-mei; ZHANG Xian-yan; YIN Dong-mei   

  1. 1、College of Horticulture, Nanjing Agricultural University, Nanjing 210095;
    2、Flower Research & Development Center, Zhejiang Academy of Agricultural Sciences, Hangzhou 311202
  • Received:2010-09-10 Online:2011-04-02 Published:2010-12-15

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Abstract: 【Objective】The purpose of this study is to detect main-effect QTL controlling tubular floret number and flower center diameter and provide some reference for molecular marker-assisted breeding of inflorescence traits in chrysanthemum.【Method】In this study, tubular floret number and flower center diameter of chrysanthemum were evaluated in 2008 and 2009, and QTL analysis was undertaken for the two traits based on the two-year phenotypic data in 142 F1 mapping population derived from a cross between chrysanthemum cultivars ‘Yuhualuoying’ and ‘Aoyunhanxiao’ and their SRAP-based genetic linkage maps.【Result】 The two traits of tubular floret number and flower center diameter showed continuous variation and significantly correlated with each other with Pearson correlation coefficient of 0.37 (P＜0.01). The QTL analysis indicated that 2 for tubular floret number and 7 for center floret number QTL were detected based on the two-year phenotypic data. These QTL scattered on 6 linkage groups, including Y1, Y2 and Y3 of ‘Yuhualuoying’ map, and A5, A13 and A19 of ‘Aoyunhanxiao’ map, with a LOD value ranging from 2.50 to 4.18 and a contribution ratio to the phenotypic variation of 6.17%-19.95% for each QTL. TfnE1Y21 and TfnE2Y21 controlling tubular floret number as well as FcdE1Y1 and FcdE2Y1 controlling flower center diameter which were mapped in the same marker interval on the linkage groups of Y21 and Y1 in 2008 and 2009 should be the same QTL and were less affected by environment, respectively. The other QTL detected in either 2008 or 2009 were remarkably affected by environment. 【Conclusion】A total of 9 QTL were mapped for tubular floret number and flower center diameter and the major QTL little affected by environment would be of great significance for the future study of molecular marker assisted breeding program in chrysanthemum.

Key words: chrysanthemum , F1 population , tubular floret number , flower center diameter , QTL analysis

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