环介导等温扩增技术检测菊花中番茄不孕病毒

doi:10.3864/j.issn.0578-1752.2010.06.024

Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (6): 1288-1294 .doi: 10.3864/j.issn.0578-1752.2010.06.024

• RESEARCH NOTES • Previous Articles Next Articles

Detection of Tomato Aspermy Virus Infecting Chrysanthemums by LAMP #br#

LIU Jia, HUANG Cong-lin, WU Zhong-yi, ZHANG Xiu-hai, WANG Yong-qin #br#

（北京市农林科学院/北京农业生物技术研究中心）

Received:2009-08-13 Revised:2009-10-09 Online:2010-03-15 Published:2010-03-15
Contact: WANG Yong-qin

Abstract

Abstract:

【Objective】 In this study, the LAMP (loop-mediated isothermal amplification) was used to detect TAV (Tomato aspermy virus). 【Method】 Using TAV CP cDNA clones as templates, the reaction conditions (such as Mg2+, dNTPs, betaine and primers concentrations) optimized and the sensitivities of LAMP and RT-PCR were compared in detecting TAV. 【Result】 The LAMP method was developed in this experiment and it was more sensitive than RT-PCR method for detecting TAV. 【Conclusion】 The LAMP as a simple and quick method can accurately detect TAV in chrysanthemum samples.

Key words: chrysanthemum')">chrysanthemum, Tomato aspermy virus, RT-PCR, loop-mediated isothermal amplification (LAMP) , detection

LIU Jia,HUANG Cong-lin,WU Zhong-yi,ZHANG Xiu-hai,WANG Yong-qin
. Detection of Tomato Aspermy Virus Infecting Chrysanthemums by LAMP #br# [J].Scientia Agricultura Sinica, 2010, 43(6): 1288-1294 .

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