Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (5): 993-999 .doi: 10.3864/j.issn.0578-1752.2010.05.014

• HORTICULTURE • Previous Articles     Next Articles

Mapping of Recessive Genic Male Sterile Restoring Gene (BrMf2) in Brassica rapa L. ssp. pekinensis

ZHANG Hui, ZHANG Shu-jiang, WU Jian, LI Fei, ZHANG Shi-fan, WANG Xiao-wu, ZHANG Xin-mei, SUN Ri-fei
   

  1. （中国农业科学院蔬菜花卉研究所）
  • Received:2009-07-10 Revised:2009-10-21 Online:2010-03-01 Published:2010-03-01
  • Contact: SUN Ri-fei

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Abstract:

【Objective】 Recessive genic male sterility (RGMS) is one of the important methods for F1 seeds production in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Identification of molecular markers linked to RGMS restoring gene not only can accelerate breeding programs, but also can provide information for cloning of RGMS gene by mapping this gene on the corresponding chromosome. 【Methods】 A segregating population was developed from a cross between a male sterile line 939A and a fertile line YQD56A. SRAP and SRAP-AFLP techniques and bulked segregant analysis (BSA) were used to screen markers linked to the RGMS restoring gene. 【Result】 Among the 1 256 primer combinations, only PM8/K4 and Me2/M49 showed polymorphism between bulks of male sterile and fertile. The polymorphic bands were named as PM8K4 and Me2M49. The distance of PM8K4 and Me2M49 was 2.98 cM and 10.92 cM, respectively. PM8K4 was subsequently mapped on linkage group A8 (Chr9) using a doubled-haploid mapping population. 【Conclusion】 The SRAP and SRAP-AFLP markers developed here can be used in breeding programs for Chinese cabbage.

Key words: Brasscia rapa L. ssp. pekinesis, recessive genic male sterile (RGMS), gene mapping, molecular marker, SRAP

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