不同淀粉组分含量小麦品种灌浆过程中淀粉去分支酶的活性及类型

doi:10.3864/j.issn.0578-1752.2010.04.024

Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (4): 850-854 .doi: 10.3864/j.issn.0578-1752.2010.04.024

• RESEARCH NOTES • Previous Articles Next Articles

Activity and Types of Starch Debranching Enzyme in Wheat Cultivars Containing Different Starch Contents During the Grain Filling Period

LIU Xia, TAN Sheng-bing, TIAN Ji-chun, WANG Xian-ze

（浙江师范大学化学与生命科学学院）

Received:2006-03-13 Revised:2006-05-16 Online:2010-02-20 Published:2010-02-20
Contact: WANG Xian-ze

Abstract

Abstract:

【Objective】 In order to provide a theoretical basis on studying the mechanism of starch debranching enzyme (DBE) further, activity differences and type of DBE during grain filling period of four wheat cultivars were studied. 【Method】Activity differences and type of DBE among Nuomai 1, Guandong 107, Jinan 16, and Lumai 21 were analyzed using native-PAGE and HPLC techniques. 【Result】 Changes of DBE activity of four cultivars had a single-peak curve, and the peak appeared in 14 d after anthesis. HPLC analysis showed that the activity of DBE to amylopectin was similar. There are two types of DBE in the wheat grains, one is isoamylase, and the other is limit dextrinase. 【Conclusion】 Though starch constitution of four cultivars is different, the DBE activity has a similar activity change of single-peak curve and the activity to amylopectin is similar in vitro.

Key words: wheat, starch, starch debranching enzyme (DBE), native-PAGE

LIU Xia,TAN Sheng-bing,TIAN Ji-chun,WANG Xian-ze
. Activity and Types of Starch Debranching Enzyme in Wheat Cultivars Containing Different Starch Contents During the Grain Filling Period
[J].Scientia Agricultura Sinica, 2010, 43(4): 850-854 .

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