与苜蓿褐斑病(CLS)抗性基因连锁的SRAP标记研究

doi:10.3864/j.issn.0578-1752.2010.02.027

Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (2): 438-442 .doi: 10.3864/j.issn.0578-1752.2010.02.027

• RESEARCH NOTES • Previous Articles

Analysis of SRAP Markers Associated with Common Leaf Spot Disease Resistance Gene in Alfalfa

WANG Yu, YUAN Qing-hua, LI Xiang-lin, GAO Jian-ming

（中国农业科学院北京畜牧兽医研究所）

Received:2009-03-27 Revised:2009-08-17 Online:2010-01-20 Published:2010-01-20
Contact: YUAN Qing-hua

Abstract

Abstract:

【Objective】 The study identified the molecular markers linked to common leaf spot disease resistance gene in alfalfa. 【Method】 With a F1 population produced by two middling resistant parents (YL0602M×SH0602M), the molecular marker associated with common leaf spot disease resistance gene was screened by BSA method and SRAP technology. 【Result】 One specific band was found between resistant and susceptible DNA pool and 20 plants that comprised the two pools. Sequencing of the fragment indicated that its length was 169 bp. This SRAP marker was named as M3E3-R169. 【Conclusion】 The coincidence rate of the existence of M3E3-R169 in 20 plants was 80%, so M3E3-R169 would be linked to common leaf spot disease resistance gene.

Key words: alfalfa, common leaf spot, SRAP markers

WANG Yu,YUAN Qing-hua,LI Xiang-lin,GAO Jian-ming
. Analysis of SRAP Markers Associated with Common Leaf Spot Disease Resistance Gene in Alfalfa
[J].Scientia Agricultura Sinica, 2010, 43(2): 438-442 .

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