Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (2): 438-442 .doi: 10.3864/j.issn.0578-1752.2010.02.027

• RESEARCH NOTES • Previous Articles    

Analysis of SRAP Markers Associated with Common Leaf Spot Disease Resistance Gene in Alfalfa

WANG Yu, YUAN Qing-hua, LI Xiang-lin, GAO Jian-ming
   

  1. （中国农业科学院北京畜牧兽医研究所）
  • Received:2009-03-27 Revised:2009-08-17 Online:2010-01-20 Published:2010-01-20
  • Contact: YUAN Qing-hua

PDF

1025

Abstract:

【Objective】 The study identified the molecular markers linked to common leaf spot disease resistance gene in alfalfa. 【Method】 With a F1 population produced by two middling resistant parents (YL0602M×SH0602M), the molecular marker associated with common leaf spot disease resistance gene was screened by BSA method and SRAP technology. 【Result】 One specific band was found between resistant and susceptible DNA pool and 20 plants that comprised the two pools. Sequencing of the fragment indicated that its length was 169 bp. This SRAP marker was named as M3E3-R169. 【Conclusion】 The coincidence rate of the existence of M3E3-R169 in 20 plants was 80%, so M3E3-R169 would be linked to common leaf spot disease resistance gene.

Key words: alfalfa, common leaf spot, SRAP markers

[1] WANG WenJuan, SHI ShangLi, KANG WenJuan, DU YuanYuan, YIN Chen. The Physiological Response of Longzhong Alfalfa to Exogenous Spermine Under Drought Stress [J]. Scientia Agricultura Sinica, 2025, 58(4): 676-691.
[2] XU XiuYuan, ZHANG HongZhi, XU LiJun, XUE Wei, NIE YingYing, GUO MingYing, LI JinXia, ZHAO YaRu, SHI MingJiang. Effects of Nitrogen Fertilization on Photosynthetic Carbon Allocation in Pasture Based on 13C Pulse-Labeling Experiments [J]. Scientia Agricultura Sinica, 2025, 58(21): 4346-4356.
[3] ZHANG Fan, YANG QingChuan. The Breeding History, Current Status and Prospects of Alfalfa [J]. Scientia Agricultura Sinica, 2025, 58(21): 4471-4481.
[4] LÜ HuanHuan, LI RuYue, LIU QingSong, XU Lei, XU YanRan, YU HaoJie, GUO ChangHong, LONG RuiCai. Cloning and Salt Tolerance Function Analysis of MsKTI3 Gene in Alfalfa [J]. Scientia Agricultura Sinica, 2025, 58(21): 4497-4511.
[5] YANG YongNian, ZENG XiangCui, LIU QingSong, LI RuYue, LONG RuiCai, CHEN Lin, WANG Xue, HE Fei, KANG JunMei, LI MingNa. Differential Proteomic Analysis of Alfalfa Seedlings Under Salt- Alkaline Stress [J]. Scientia Agricultura Sinica, 2025, 58(21): 4512-4527.
[6] HUANG HongMei, WANG SiQi, YANG QingChuan, GUO ChangHong, WANG Xue. Phosphate Transporter MsPT5 Regulates Phosphate Uptake and Utilization in Alfalfa [J]. Scientia Agricultura Sinica, 2025, 58(21): 4544-4556.
[7] BAO MingFang, QIN Yan, CHEN CaiJin, ZHANG ShangPei, ZHANG GuoHui, SHA XiaoDi. Evaluation of 111 Alfalfa Germplasm Resources for Seedling Phenotypic Drought Tolerance Characterization [J]. Scientia Agricultura Sinica, 2025, 58(19): 3825-3836.
[8] LI RuoHong, MA Wen, ZHAO ShiQiang, MAO PeiSheng. Antioxidant Physiology and Hormonal Response Pattern of Embryonic Root Mitochondria During Imbibition of Aging Seeds of Alfalfa [J]. Scientia Agricultura Sinica, 2025, 58(19): 4014-4025.
[9] ZENG XiangCui, YANG YongNian, LI RuYue, JIANG XueQian, JIANG Xu, XU YanRan, LIU ZhongKuan, LONG RuiCai, KANG JunMei, YANG QingChuan, LI MingNa. Identification of Alfalfa (Medicago sativa) MsCEP Genes and Functional Analysis of Its Regulation in Root Growth and Development [J]. Scientia Agricultura Sinica, 2024, 57(24): 4839-4853.
[10] CHEN FeiEr, ZHANG ZhiPeng, JIANG QingXue, MA Lin, WANG XueMin. Cloning and Biological Function Verification of Alfalfa MsSPL17 [J]. Scientia Agricultura Sinica, 2024, 57(17): 3335-3349.
[11] ZHAO JianTao, YANG KaiXin, WANG XuZhe, MA ChunHui, ZHANG QianBing. Effect of Phosphorus Application on Physiological Parameters and Antioxidant Capacity in Alfalfa Leaves [J]. Scientia Agricultura Sinica, 2023, 56(3): 453-465.
[12] SU Qian,DU WenXuan,MA Lin,XIA YaYing,LI Xue,QI Zhi,PANG YongZhen. Cloning and Functional Analyses of MsCIPK2 in Medicago sativa [J]. Scientia Agricultura Sinica, 2022, 55(19): 3697-3709.
[13] ZHANG YunXiu,JIANG Xu,WEI ChunXue,JIANG XueQian,LU DongYu,LONG RuiCai,YANG QingChuan,WANG Zhen,KANG JunMei. The Functional Analysis of High Mobility Group MsHMG-Y Involved in Flowering Regulation in Medicago sativa L. [J]. Scientia Agricultura Sinica, 2022, 55(16): 3082-3092.
[14] MA Lin,WEN HongYu,WANG XueMin,GAO HongWen,PANG YongZhen. Cloning and Function Analysis of MsMAX2 Gene in Alfalfa (Medicago sativa L.) [J]. Scientia Agricultura Sinica, 2021, 54(19): 4061-4069.
[15] ZeMin LI,Chen ZHANG,ChongYu ZHANG,GuiGuo ZHANG. The Relationship Between Nutrients and Biological Yield of Different Varieties of Alfalfa [J]. Scientia Agricultura Sinica, 2020, 53(6): 1269-1277.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd