Abstract:

【Objective】 An experiment was conducted using a total of 320 one day old AA male broiler chickens to investigate the effect of dietary supplemented with glutamine and aspartic on fatty traits, blood fat levels, adipogenesis genes mRNA level in liver and coelo-grease and the possible mechanism of their function to lipid metabolism of broiler. 【Method】 The broilers were randomly tested by one of four treatments in a completely randomized design of treatments. They were fed by basic corn-soybean diet and basic diet supplemented with 1% glutamine and 1% aspartic, respectively, for a duration of 42 days. The rate of abdominal fat, the breadth of subcutaneous fat and the thickness of intermuscular fat was measured by vernier caliper. The levels of serum TG, TC, HDL-C were determined by ultraviolet spectrophotometer and the adipogenesis genes (ACC, FAS, CPT-1, LPL, PPARα) mRNA levels in liver and abdominal fat tissue were detected by RT-PCR technology after slaughtered. 【Result】 The addition of a mixed diet with aspartic acid and glutamine significantly improved the broiler weight gain (P＜0.05) but not for feed conversion rate(P＞0.05), significantly increased the width of intermuscular fat and subcutaneous fat thickness (P＜0.05), but not for the percentage of abdominal fat and percentage of dressing (P＞0.05). Supplemented with glutamine and the aspartic solo significantly reduced the rate of abdominal fat compared to control group (P＜0.05), the two substances significantly increased in subcutaneous fat thickness and the width of intermuscular fat respectively (P＜0.05). The addition of glutamine and aspartic had impact on lipid metabolism by significantly reducting the level of TG and HDL-C in blood (P＜0.05), they also raised the content of TC level significantly at the same time (P＜0.05). ACC, FAS, CPT-1, PPARα mRNA level of the three trial groups was significantly lower than the control group (P＜0.05); the glutamine group LPL mRNA level increased significantly in liver (P＜0.05). For adipose tissue, compared to the control group, the transcription expression of PPARα significantly increased in all three trial groups (P＜0.05), glutamine group ACC mRNA level significantly decreased (P＜0.05), FAS, LPL gene transcription level significantly reduced in the mixed group lower (P＜0.05) but CPT-1 mRNA level significantly increased (P＜0.05). The others did not change significantly (P＞0.05). 【Conclusions】 The results indicate that the addition of glutamine and aspartate in dietary can raise growth performance of broiler, reduce abdominal fat deposition and improve the carcass fatty traits to a certain extent.

Key words: glutamine')">glutamine, aspartate, broiler, fatty traits, adipogenesis genes