朗德鹅,填饲,产肝性能,细胞形态,脂肪酸组分,基因表达," /> 朗德鹅,填饲,产肝性能,细胞形态,脂肪酸组分,基因表达,"/> Lande goose,overfeeding,liver performance,cell histology,fatty acid profile,expression of genes
,"/> <font face="Verdana">Measurement of Liver Performance, Histology and Adipogenic Genes Transcriptional Expresstion of Landes Goose Liver in Response to Overfeeding#br# </font>

Scientia Agricultura Sinica ›› 2009, Vol. 42 ›› Issue (7): 2523-2530 .doi: 10.3864/j.issn.0578-1752.2009.07.033

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Measurement of Liver Performance, Histology and Adipogenic Genes Transcriptional Expresstion of Landes Goose Liver in Response to Overfeeding#br#

SU Sheng-yan, LI Qi-fa, CHEN Rui, ZENG Yi, ZHU Hong-sheng, ZHANG Xiang, WANG Yan-ping,XIE Zhuang, WU Song-qing, LIU Yu-di#br#   

  1. (南京农业大学动物科技学院)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-10 Published:2009-07-10
  • Contact: XIE Zhuang

Abstract: 【Objective】 An experiment was conducted to study the candidate gene and early breeding projects of the fattty liver of Landes goose. 【Method】 Histological examination and analysis of fatty acids of the fatty liver were done using H & E stain and gas chromatography, respectively. The mRNA expression level of lipogenic genes was detected by real-time PCR. CT was used to investigate the fat deposition of living Landes goose. 【Result】 The result shown that compared to control group, overfeeding increased the liver weight, liver index, serum ALT, TG, Che, HDL levels and changed the positive ratio of liver and spleen to negative. Swelling of hepatocyte were observed within the cytoplasm of liver cells in goose treated with overfeeding compared to control. The values for the SFA and PUFA were significantly higher in control group than in overfeeding group. Higher values (P<0.05) for MUFA and UFA in liver were found in goose in overfeeding group. Compared to the control group, the mRNA expression level of C/EBPα、ACCα increased in overfeeding group (P<0.05). The positive correlation between the transcriptional level of ACCα and serum TG was observed (P<0.01). 【Conclusion】 overfeeding can make the hepatocyte swelled and change the composition of fatty acids. The mRNA expression level of lipogenic genes increased, such as C/EBPα, ACCα. CT can be used to investigate the fat deposition of living Landes goose.

Key words: Lande goose')">Lande goose, overfeeding, liver performance, cell histology, fatty acid profile, expression of genes

[1] WANG Bao-Wei-1, SHU Chang-Ping-2, GE Wen-Hua-1, YUE Bin-1, ZHANG Ming-Ai-1, JIANG Yang-3. Deposition of Foie Gras Goose Body Fat, Composition of Lipid and Metabolism of Lipid in Overfeeding Period [J]. Scientia Agricultura Sinica, 2014, 47(8): 1600-1610.
[2] SHU Chang-Ping, WANG Bao-Wei, LI Zhen, GE Wen-Hua, ZHANG Ming-Ai, YUE Bin. Fatty Acids Deposition and FAS mRNA Expression Abundance in Liver Tissue of Overfeeding Goose [J]. Scientia Agricultura Sinica, 2012, 45(10): 2002-2011.
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