Abstract: Abstract:【Objective】 To establish a method of isolating, culturing and amplification bovine bone marrow mesenchymal stem cells(BMSCs)in vitro and to explore the effect of age on the proliferation of bovine BMSCs. 【Method】 BMSCs were isolated and purified with adherence plasticity method, passage amplified, measured growth curve and attachment rate, morphological observations of BMSCs. 【Result】 BMSCs that are in low abundance can grow quickly when cultured in vitro. Calf BMSCs than adult cattle easy isolation and purification, adherence rate and reproductive activity significant super adult cattle. 【Conclusion】 Calf were more appropriate than those of adult cattle for BMSCs extraction. Between the age and the biological characterstics of bovine BMSCs is intimate relationship, the proliferation capability and activity of bovine BMSCs decrease with age. The method for isolation and culture of BMSCs has been successfully established in this study, some biological features were observed. It found a base for further investigation and using of BMSCs.

Key words: Bovine, Age, Bone marrow mesenchymal stem cells, Biological characteristics, Proliferation